All reagents were purchased from Thermo Fischer Scientific (Waltham, MA, USA) unless otherwise stated. Chronolume, used for the detection of secreted ATP, was purchased from Chrono‐log Corporation (Havertown, PA, USA). The CLEC‐2 activating antibody for human was purchased from R & D Systems (Minneapolis, MN, USA). The CLEC‐2 activating antibody (clone 17D9) for mouse was purchased from BioLegend (San Diego, CA, USA) and the donkey anti‐rat (DAR) IgG was purchased from Novex (Wadsworth, OH, USA). Anti‐pSyk Y352 (Y346 in mouse) and anti‐pSyk Y348 (Y342 in mice) were purchased from Abcam (Cambridge, England). Anti‐Syk and anti‐PLCγ2 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti‐pSyk Y525/526 (mouse Y519/520), anti‐pPLCγ2 Y1217, and anti‐pLAT Y191 were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti‐LAT was purchased from EMD Millipore (Burlington, MA, USA). Anti‐human CD32 antibody, clone IV.3 was obtained from STEMCELL Technologies (Vancouver, BC, Canada). Odyssey blocking buffer and secondary antibodies IRDye 800CW goat anti‐rabbit and IRDye 680LT goat anti‐mouse were purchased from Li‐Cor (Lincoln, NE, USA).
Anti syk and anti plcγ2
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-Syk and anti-PLCγ2 are antibodies produced by Santa Cruz Biotechnology. They are used by researchers to detect and study the Syk and PLCγ2 proteins, which play important roles in cellular signaling and immune function.
Automatically generated - may contain errors
Lab products found in correlation
Irdye 680lt goat anti mouse, by LI COR (2 mentions)
Donkey anti rat igg, by Thermo Fisher Scientific (2 mentions)
Irdye 800cw goat anti rabbit, by LI COR (2 mentions)
Anti pplcγ2 y1217, by Cell Signaling Technology (2 mentions)
Chronolume, by Chrono-Log (2 mentions)
Anti psyk y352 y346 in mouse, by Abcam (1 mentions)
Anti lat, by Merck Group (1 mentions)
Anti human cd32 antibody clone 4 3, by STEMCELL (1 mentions)
Anti psyk y525 526 mouse y519 520, by Cell Signaling Technology (1 mentions)
Anti psyk y348 y342 in mice, by Abcam (1 mentions)
Anti pplcγ2 y759, by Cell Signaling Technology (1 mentions)
Anti slp 76, by Cell Signaling Technology (1 mentions)
2 protocols using anti syk and anti plcγ2
1
CLEC-2 Signaling Pathway Activation
2
CLEC-2 Signaling Pathway Activation
Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents were purchased from Thermo Fischer Scientific unless otherwise noted. Rhodocytin was isolated as previously described.
36 (link)
Chronolume, used for the detection of secreted adenosine triphosphate (ATP), was purchased from Chrono-log Corp. (Havertown, PA). The CLEC-2-activating antibody (17D/CLEC-2) was purchased from Biolegend (San Diego, CA), while donkey anti-rat (DAR) IgG was purchased from Novex (Frederick, MD). Anti-pSyk Y352, anti-pSyk Y525/Y526, anti-pPLCγ2 Y759, anti-pPLCγ2 Y1217, and anti-SLP76 were all purchased from Cell Signaling Technology (Beverly, MA). Please note that Syk pY sites listed correspond to human Syk as listed by the manufacturer. The numbering of mouse pY sites is different but will be noted in the paper. Anti-Syk and anti-PLCγ2 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-pSLP-76 Y128 was purchased from BD Transduction (San Jose, CA). Odyssey blocking buffer and secondary antibodies IRDye 800CW goat anti-rabbit and IRDye 680LT goat anti-mouse were purchased from Li-Cor (Lincoln, NE). Please see the “major resources” table for further antibody information.
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All reagents were purchased from Thermo Fischer Scientific unless otherwise noted. Rhodocytin was isolated as previously described.
36 (link)
Chronolume, used for the detection of secreted adenosine triphosphate (ATP), was purchased from Chrono-log Corp. (Havertown, PA). The CLEC-2-activating antibody (17D/CLEC-2) was purchased from Biolegend (San Diego, CA), while donkey anti-rat (DAR) IgG was purchased from Novex (Frederick, MD). Anti-pSyk Y352, anti-pSyk Y525/Y526, anti-pPLCγ2 Y759, anti-pPLCγ2 Y1217, and anti-SLP76 were all purchased from Cell Signaling Technology (Beverly, MA). Please note that Syk pY sites listed correspond to human Syk as listed by the manufacturer. The numbering of mouse pY sites is different but will be noted in the paper. Anti-Syk and anti-PLCγ2 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-pSLP-76 Y128 was purchased from BD Transduction (San Jose, CA). Odyssey blocking buffer and secondary antibodies IRDye 800CW goat anti-rabbit and IRDye 680LT goat anti-mouse were purchased from Li-Cor (Lincoln, NE). Please see the “major resources” table for further antibody information.
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