Megal

Leukemia cell lines, LOUCY, MEGAL, and OCI-AML3 were purchased from DSMZ (Braunschweig, Germany). K562, HL60, and HEL cells were obtained from JCRB cell bank (Ibaraki, Osaka). These cell lines were authenticated using STR typing by DSMZ or JCRB cell bank, and also the mycoplasma status was checked by them. LOUCY, MEGAL, and HL60 cells were cultured in RPMI1640 medium (Sigma) supplemented with 20% FBS (Sigma). K562 and HEL cells were cultured in RPMI1640 medium supplemented with 10% FBS. OCI-AML3 was cultured in alpha-MEM medium (WAKO) supplemented with 20% FBS. EB3 ES cells (Niwa et al., 2002 (link); Ogawa et al., 2004 (link)), E14tg2a ES cells (Hooper et al., 1987 (link)) and their derivatives, and HeLa cells were cultured as described previously (Oka et al., 2016 (link)).

LOUCY (T-cell leukemia), MEGAL (acute megakaryoblastic leukemia), FKH-1 (acute myelocytic leukemia), OCI-AML1 (acute myeloid leukemia), and MOLM-13 (acute myeloid leukemia) cell lines were purchased from the Leibniz Institute - DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany). For details see Supplementary Table 1. HCT-116 cells were a gift from Dr. Denis Lafontaine (Institute of Molecular Biology and Medicine, Université Libre de Bruxelles, Gosselies, Belgium). Cell lines were maintained as detailed in the Online Supplementary Methods.