Cd10 56c6

Manufactured by Agilent Technologies

Sourced in France

The CD10 (56C6) is a laboratory equipment product from Agilent Technologies. It is a sensitive and specific monoclonal antibody used for the detection of CD10 antigen, which is expressed on the surface of various cell types, including B-cell precursors and granulocytes. The CD10 (56C6) can be used in immunohistochemical and flow cytometric applications to identify and characterize cells of interest.

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CD10 (clone 56C6, (12 citations)

3 protocols using cd10 56c6

Immunohistochemical Profiling of Tumor Markers

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IHC analysis was performed on representative 3–5 μm formalin-fixed paraffin-embedded (FFPE) whole tumor tissue sections. Staining for routinely used markers including Pax8 (10336-1-A P, 1:1000; Proteintech), CA9 (11071-1-AP, 1:100; Thermo Fisher), CD10 (56C6, 1:40; Dako), CK7 (M7018- OV-TL 12/30, 1:100; Dako), CK20 (7019-Ks20.8, 1:50; Dako), CD117 (A4502, 1:700; Dako), Melan A (7196-A103, 1;200; Dako), cathepsin-K (37259-3F9, 1:100; Abcam), FH (100743-J13, 1:1500; Santa Cruz), SDHB (14714-21A11, 1:100; Abcam), TFE3 (MRQ-37, 1:200; Cell Marque), TFEB (166736, 1:100; Santa Cruz), vimentin (M0725, 1:75; Dako), phospho-S6 (Ser240/244) (p-S6) (5364-D68F8, 1:300; Cell Signaling Technology) and phospho-4E-BP1 (Thr37/46) (p-4EBP1) (236B4, 1:800; Cell Signaling Technology) was performed using a Dako automated system (Agilent).
Immunoreactivity was interpreted as “negative” if < 5% tumor cells stained positively. P-S6 and p-4EBP1 expression was determined based on the percentage of tumor cells staining positive and the intensity of expression, which were multiplied.

Kapur P., Gao M., Zhong H., Chintalapati S., Mitui M., Barnes S., Zhou Q., Miyata J., Carrillo D., Malladi V., Rakheja D., Pedrosa I., Xu L., Kinch L, & Brugarolas J. (2021). Germline and sporadic mTOR pathway mutations in low-grade oncocytic tumor of the kidney. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 35(3), 333-343.

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Immunohistochemical Staining Protocol for DLBCL

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The immunohistochemical staining of DLBCLs was performed according to the protocol [18] (link). The immunohistochemistrical antibodies were listed as follows: CD10 (56C6, Dako), Bcl6 (M7211, Dako), MUM1 (MUM1p, Dako), Ki67 (MB1, Dako), P50 (sc-8414, Santa Cruz), P65 (#4764, Cell Signaling) and Notch2 (ab52302, Abcam). For Ki67 [26] (link) and Notch2 [27] (link), cases were considered to be positive when 25% or 20% of lymphoma cells were stained, respectively. For the rest antibodies, we defined 30% as the cut-off [28] (link), [29] (link).

Zhang X., Shi Y., Weng Y., Lai Q., Luo T., Zhao J., Ren G., Li W., Pan H., Ke Y., Zhang W., He Q., Wang Q, & Zhou R. (2014). The Truncate Mutation of Notch2 Enhances Cell Proliferation through Activating the NF-κB Signal Pathway in the Diffuse Large B-Cell Lymphomas. PLoS ONE, 9(10), e108747.

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Immunocytochemical Profiling of Tissue Samples

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Following SRH imaging, the sample were recovered from between the coverslips and fixed in 4% formalin for 24 hours. The specimens were then included in a conventional pathology workflow comprising paraffin embedding, sectioning and HE staining. Immunochemistry was performed on the Benchmark Ventana autostainer (Ventana Medical Systems SA, Illkirch, France) with routinely used antibodies targeting AE1-AE3 (Polyclonal, Roche), Bcl6 (PG-B6p, Dako), CD10 (56C6, Dako), CD20 (L26, Cliniscience), CD3 (Polyclonal, Dako), CD56/NCAM (123C3, Roche), CDX2 (DAK-CDX2, Dako), Chromogranin (DAK-A3, Dako), CK20 (Ks20.8, Dako), CK5/6 (DS/16B4, Dako), CK7 (OV.TL12/30, Menarini), EGFR (3C6, Roche), EMA (E29, Dako), Filamin (PM6/317, Interchim), FSH (Polyclonal, Roche), GATA3 (L50-823, Roche), GFAP (6F2, Dako), IDH1 R132H (H09, Clinisciences), Ki67 (Mib-1, Dako), MUM1 (MUM1P, Dako), NeuN (A60, Millipore), Neurofilament (2F11, Sigma-Aldrich), OLIG2 (EP112, Diagomix), P63 (4A4, Roche), PS100 (Polyclonal, Dako), RP (1/E2, Roche), SSTR2 (UMB1, Abcam), Synaptophysin (DAK-SYNAP, Dako), TTF1 (8G7G3/1, Dako). The slides were scanned using the Hamamatsu Nanozoomer system 2.0-HT and the presented images were obtained using the NDP.view2 viewing software (ver 2.6.17).

Appay R., Sarri B., Heuke S., Boissonneau S., Liu C., Dougy E., Daniel L., Scavarda D., Dufour H., Figarella-Branger D, & Rigneault H. (2023). Live Stimulated Raman Histology for the Near-Instant Assessment of Central Nervous System Samples. The journal of physical chemistry. B, 127(16).

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