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5 protocols using chrysoeriol

1

Phenolic Compound Analysis Protocol

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Acetonitrile and formic acid (both of them LC/MS grade) were purchased from Merck (Darmstadt, Germany). Standards of phenolic compounds (gallic acid, protocatechuic acid, p-hydroxybenzoic acid, gentisic acid, caffeic acid, p-coumaric acid, apigenin 7-O-glucoside, luteolin, apigenin, chrysoeriol, quercetin 3-O-galactoside, isorhamnetin 3-O-glucoside, quercetin, and kaempferol) were supplied by Sigma Aldrich (Steinheim, Germany). The 3.5-dinitrosalicylic acid was also supplied by Sigma Aldrich (Steinheim, Germany). Syringe filters (25 mm, nylon membrane, 0.45 µm) were purchased from PSI Lab (Belgrade, Serbia). MRS broth and agar were supplied by HiMedia (Mumbai, India).
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Analytical Standards for Cannabis Compounds

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cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabigerol (CBG), and cannabidiol (CBD) standard solutions (1 mg/mL in methanol or acetonitrile) were purchased from Cerilliant (Round Rock, TX, USA). Chrysoeriol was purchased from Sigma-Aldrich (Milan, Italy), while canniprene was kindly provided by Dr. Federica Pollastro of the Department of Drug Sciences of the University of Piemonte Orientale (Novara, Italy). Formic acid (HCOOH) and HPLC-grade solvents, including EtOH, MeOH, ACN, acetone, EtOAc, n-hexane, DCM, ethyl ether and toluene, were from VWR (Milan, Italy). H2O was purified by using a Milli-Q Plus185 system from Millipore (Milford, MA, USA).
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Phenolic Compound Analytical Standards

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Acetonitrile and formic acid (both of analytical grade) were purchased from Merck (Darmstadt, Germany). Analytical standards of phenolic compounds (chlorogenic acid, esculin, rutin, narcissin, isorhamnetin-3-O-glucoside, rosmarinic acid, apigenin, chrysoeriol, and kaempferide) were supplied by Sigma-Aldrich (Steinheim, Germany).
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Phytochemical Analysis of Plant Extracts

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Sample, Methanol (Merck), Chrysoeriol (Sigma Aldrich), Cirsimaritin (Sigma Aldrich), Eriodictyol (Sigma Aldrich), Luteolin (Sigma Aldrich), Rutin (Sigma Aldrich), Salvigenin (Sigma Aldrich), Thymoquinone (Sigma Aldrich), Quercetin (Sigma Aldrich), Apigenin (Sigma Aldrich), and 5-O-Methyl-Luteolin (Sigma Aldrich), 1,1-Diphenyl-2-Picryhydrazil (Sigma Aldrich).
Pharmacognosy Journal, Vol 12, Issue 6(Suppl), Nov-Dec, 2020
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5

Quantification of Phenolic Compounds in Botanical Extracts

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Acetonitrile and acetic acid (both MS grade), methanol (HPLC grade), sodium carbonate, potassium chloride, hydrochloric acid and Folin-Ciocalteu reagent were purchased from Merck (Darmstadt, Germany). The SPE cartridges used for extraction and concentration of samples were Strata C18-E (500 mg/3 mL) obtained from Phenomenex (Torrance, CA). 2,2-Diphenyl-1picrylhydrazyl (DPPH) and phenolic compounds standards (protocatechuic acid -PrA, aesculin, 5-Ocaffeoylquinic acid -CQA, p-hydroxybenzoic acid -HBA, gentisic acid, p-hydroxyphenylacetic acid -HPA, vanillic acid -VA, aesculetin, caffeic acid -CA, rutin -Rut, p-coumaric acid -CouA, quercetin 3-O-glucoside, sinapic acid -SA, naringin -Nar, ferulic acid -FA, quercetin 3-O-rhamnoside -Que-3 rha, coniferyl aldehyde, quercetin -Que, eriodictyol -Erio, luteolin -Lut, naringenin -Narn, apigenin -Api, genistein -Gen, kaempferol -Kfrl, pinobanksin , isorhamnetin, chrysoeriol, genkwanin -Genkw, chrysin -Chry, pinocembrin -Pin, kaempferide -Kfrd, galangin -Gal, and acacetin -Aca) were supplied by Sigma-Aldrich (Steinheim, Germany).
Ultrapure water (ThermoFisher TKA MicroPure water purification system, 0.055 μS/cm) was used to prepare standard solutions and blanks. Syringe filters (13 mm, PTFE membrane 0.45 μm) were purchased from Supelco (Bellefonte, PA). Filter paper (Whatman No. 1) was supplied by Merck (Darmstadt, Germany).
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