Ast kit

Serum concentrations of creatinine, urea nitrogen, angiotensin II, aldosterone, BNP, ALT, AST, KIM-1, NGAL, RBP4 were detected by using Creatinine Kit (BC4910, Solarbio), Blood Urea Nitrogen Kit (BC1535, Solarbio), Angiotensin II ELISA kit (ml002228, Mlbio), Aldosterone ELISA kit (ml037530, Mlbio), Brain Natriuretic Peptide EIA Kit (ml037594, Mlbio), ALT Kit (BC1555, Solarbio), AST Kit (BC1565, Solarbio), Mouse KIM-1 ELISA Kit (BC4910, Solarbio), Mouse NGAL ELISA Kit (ml002075, Mlbio), Mouse RBP4 ELISA Kit (ARG81756, Arigo). Plasma concentrations of IL-1β, IL-6 and TNF-α were measured by Mouse IL-1β ELISA Kit (ml301814, Mlbio), Mouse IL-6 ELISA Kit (ab222503, Abcam), Mouse TNF-α ELISA Kit (ab208348, Abcam). Serum levels of retinol and retinoic acid were measured by liquid chromatography/mass spectrometry analysis following the previous methods 24 (link), 25 (link).

AFB1 was purchased from Macklin.D ihydromyricetin was purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China) BCA Protein Concentration Assay Kit, CAT Kit, MDA Kit, SOD Kit, ALT Kit, GPX Kit, GST Kit and AST Kit were purchased from Solarbio Biotechnology Co., Ltd. (Beijing, China). Electronic balance was purchased from Shanghai Jingtian Electronic Instrument Co., Ltd.(Shanghai, China).model FA2104A. The 1- -16K cryocentrifuge was purchased from Shanghai Trading Co., Ltd. (Shanghai, China). model D37520. H&E staining kit, Masson staining kit and TUNEL apoptosis assay kit. The 16K freezing centrifuge was purchased from Shanghai Trading Co., Ltd. (Shanghai, China). model D37520. H&E staining kit, Masson staining kit, and TUNEL apoptosis detection kit were purchased from Beyotime Biotechnology Co. (Shanghai, China). Rabbit anti-mouse, Nrf2, Keap1, Bax, Bcl-2, HO-1, Caspase-3, Cleaved-Caspase-3, and GAPDH monoclonal antibodies were purchased from Abcam (Cambridge, UK). All other chemical reagents used in the experiments were of analytical grade provided by Beijing Chemical Factory (Beijing, China).