Washed mouse platelets were pretreated with vehicle or different concentrations of isaridin E for 30 min at 37 °C, followed by stimulation with 50 μM ADP for 5 min. Platelets were then stained with FITC-conjugated anti-CD41 antibody (133904, Biolegend, San Diego, CA, USA) and PE-conjugated anti-CD62p antibody (148306, Biolegend, San Diego, CA, USA) for 20 min at 25 °C in the dark. Then, platelets were fixed in 1% paraformaldehyde solution and analyzed with a CytoFLEX flow cytometer (Beckman Coulter, Brea, CA, USA).
Pe conjugated anti cd62p antibody
Manufactured by BioLegend
Sourced in United States
The PE-conjugated anti-CD62p antibody is a laboratory reagent that can be used to detect and quantify the expression of CD62p, also known as P-selectin, on the surface of cells. CD62p is a cell adhesion molecule that plays a role in the inflammatory response and is expressed on the surface of activated platelets and endothelial cells. The PE (phycoerythrin) fluorescent dye is conjugated to the antibody, allowing for the detection and quantification of CD62p-positive cells using flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
2 protocols using pe conjugated anti cd62p antibody
1
Isaridin E Inhibits Platelet Activation
2
Platelet Activation Dynamics in PRP
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Platelet rich plasma (PRP) was prepared by collecting the upper fraction after centrifugation of blood samples for 10 minutes at 130g. Sample were diluted 1:20 in phosphate buffer saline (PBS) and incubated for 20 minutes with PE-conjugated anti-CD62P antibody (BioLegend, San-Diego, CA), and FITC conjugated anti-PAC1 antibody (BD Bioscience, San Jose, CA), either with or without 10 μM ADP (Molab, Langenfeld, Germany).
Samples were then diluted x5 with PBS and analyzed by flow cytometry.
Data analysis was performed using the FlowJo software. Both mean fluorescence intensity (MFI) and percent positive cells were calculated.
Immature platelet fraction (IPF) was assessed with the XE-5000 hematology analyzer (Sysmex UK Ltd., Milton Keynes, UK). Immature platelet numbers (IP) were calculated based on the IPF and the total platelet count on the study date.
Samples were then diluted x5 with PBS and analyzed by flow cytometry.
Data analysis was performed using the FlowJo software. Both mean fluorescence intensity (MFI) and percent positive cells were calculated.
Immature platelet fraction (IPF) was assessed with the XE-5000 hematology analyzer (Sysmex UK Ltd., Milton Keynes, UK). Immature platelet numbers (IP) were calculated based on the IPF and the total platelet count on the study date.
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