Quantifluor dsdna detection kit

Manufactured by Promega

The QuantiFluor dsDNA Detection Kit is a fluorescence-based assay for quantifying double-stranded DNA (dsDNA) in solution. It provides a sensitive and accurate method for measuring dsDNA concentrations without the need for extensive sample preparation.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Non radioactive cytotoxicity assay kit, by Promega (1 mentions) Sarkosyl, by Merck Group (1 mentions) Murinetnf α elisa kit, by BioLegend (1 mentions) Penicillin streptomycin pen strep, by Thermo Fisher Scientific (1 mentions) Murine il 2 elisa kit, by BioLegend (1 mentions) Rmgm csf, by Thermo Fisher Scientific (1 mentions) Ripa buffer, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Anitrogen cavitation vessel, by Parr (1 mentions)

2 protocols using quantifluor dsdna detection kit

Cytotoxicity and DNA Quantification

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Supernatants from stimulated cells were mixed with the substrate mix at a 1:1 ratio and incubated for 15 min at room temperature. The percentage cytotoxicity was calculated using the OD measurements at 490 nm, according to the instructions of Non-Radioactive Cytotoxicity Assay Kit (Promega, catalog # G1780). The positive control (100% cytotoxicity) for the assay was prepared by incubating the cells with Triton X-100 at 37°C for 15 min.
DNA in the HHMX-stimulated supernatants of THP1 dual reporter cells was quantified using the QuantiFluor dsDNA Detection Kit from Promega (catalog # E2670) according to the manufacturer’s instructions.

Temizoz B., Shibahara T., Hioki K., Hayashi T., Kobiyama K., Lee M.S., Surucu N., Sag E., Kumanogoh A., Yamamoto M., Gursel M., Ozen S., Kuroda E., Coban C, & Ishii K.J. (2024). 5,6-dimethylxanthenone-4-acetic acid (DMXAA), a partial STING agonist, competes for human STING activation. Frontiers in Immunology, 15, 1353336.

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Isolation and Characterization of P. aeruginosa

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Gram-negative bacteria of P. aeruginosa (PA-103) were
purchased from ATCC (29260™). HBSS (without Ca²⁺,
Mg²⁺ and Phenol red) was purchased from Corning (Inc, NY). A
nitrogen cavitation vessel was purchased from Parr instrument (Moline, IL).
Sarkosyl and RIPA buffer were purchased from Sigma-Aldrich (St. Louis, MO, USA).
DiD (3H-Indolium,
2-(5-(1,3-dihydro-3,3-dimethyl-1-octadecyl-2H-indol-2-ylidene)-1,3-pentadienyl)-3,3-dimethy
l-1-octadecyl-, perchlorate) [Ex(640nm)\Em(670nm)], DAPI, Penicillin
streptomycin (pen strep) and glutamine (100x) were purchased from Life
Technologies (Grand Island, NY). A QuantiFluor dsDNA detection kit was purchased
from Promega. Pierce™ BCA protein assay kit was purchased from Thermo
Fisher Scientific. rmGM-CSF, anti-CD11c Abs, anti-CD40 Abs, anti-CD80 Abs,
anti-CD86 Abs, murine IL-6 ELISA kit, murine IL-1β ELISA kit and murine
TNF-α ELISA kit, murine IL-12p70 ELISA kit and murine IL-2 ELISA kit were
purchased from Biolegend.

Wang S., Gao J., Li M., Wang L, & Wang Z. (2018). A Facile Approach for Development of a Vaccine Made of Bacterial Double-layered Membrane Vesicles (DMVs). Biomaterials, 187, 28-38.

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