Gelatin type A from porcine skin (gel strength ~300 g bloom), 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium-chloride (DMTMM), chitosan, glacial acetic acid, 2,2-diphenyl-1-picrilhydrazyl (DPPH), ferric chloride (III) hexahydrate, and 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma Aldrich (Milano, Italy) and used without any further purification. Phosphate buffer saline (PBS) 10× was purchased from VWR. DHICA and MeDHICA were prepared according to a procedure previously developed [36 (link),37 (link)].
The UV–Vis spectra were recorded on a Jasco V-730 Spectrophotometer (Lecco, Italy).
HPLC analyses were performed on an Agilent 1100 binary pump instrument (Agilent Technologies, Milan, Italy) equipped with a SPD-10AV VP UV–visible detector using an octadecylsilane-coated column, 250 mm × 4.6 mm, 5 μm particle size (Phenomenex Sphereclone ODS, Bologna, Italy) at 0.7 mL/min. Detection wavelength was set at 300 nm. Eluant system: 1% formic acid:acetonitrile, 85:15 v/v.
The UV–Vis spectra were recorded on a Jasco V-730 Spectrophotometer (Lecco, Italy).
HPLC analyses were performed on an Agilent 1100 binary pump instrument (Agilent Technologies, Milan, Italy) equipped with a SPD-10AV VP UV–visible detector using an octadecylsilane-coated column, 250 mm × 4.6 mm, 5 μm particle size (Phenomenex Sphereclone ODS, Bologna, Italy) at 0.7 mL/min. Detection wavelength was set at 300 nm. Eluant system: 1% formic acid:acetonitrile, 85:15 v/v.