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T gsh gssg test kit

Manufactured by Nanjing Jiancheng
Sourced in China

The T-GSH/GSSG Test Kit is a laboratory equipment product that measures the total glutathione (T-GSH) and oxidized glutathione (GSSG) levels in biological samples. It provides a quantitative assessment of these important antioxidant molecules.

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2 protocols using t gsh gssg test kit

1

Glutathione Redox Status Quantification

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This assay was performed according to the instructions of a total Glutathione-Oxidized Glutathione (T-GSH/GSSG) Test Kit (Nanjing Jiancheng Institute of Biological Engineering, A061-1-1). The standard tube was filled with GSH and GSSG standards, and the assay tube was filled with the sample to be tested. The rest of the treatment was the same for both tubes.
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2

Evaluation of Glutaredoxin-1 Biomarkers

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Grx1 was purchased from ProSpec, Israel; carnosine was acquired from Sigma, USA; the ELISA kit for human and rat Grx1 determination was from Shanghai Wu Hao Economic, Ltd. The Redox Western blot kit (S7150) was purchased from Millipore; the antibodies against Grx1, GR, MMP-2, MMP-9, and p-NF-kB were acquired from Abcam, UK, and ECL Luminous Fluid from Thermo, USA. The T-GSH/GSSG test kit, glutathione S-transferase (GST), and total sulfhydryl (-SH) test kit were purchased from Nanjing Jiancheng, China, and the Mini RNA Isolation kit TM (R1030) and Titan TM One-Tube RT-PCR System from Roche.
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