Globulins

SHLI and its two intermediate fractions (F1, the extract of Scutellariae Radix; F2, the extract of Lonicerae Japonicae Flos and Fructus Forsythiae) were prepared by Duoduo Pharmaceutical Co., Ltd. (Jiamusi, Heilongjiang, China) according to the Chinese Pharmacopoeia¹ . Compound 48/80 (C48/80), propranolol, CV3988, triprolidine, globulins, cimetidine, and SB290157 were purchased from Sigma-Aldrich (St Louis, MO, USA). PMX53 was from GL Biochem Ltd. (Shanghai, China). Mouse tIgE ELISA kit was from Biolegend Co. (San Diego, CA, USA). Rehydragel^® aluminum adjuvant was from General Chemical (Parsippany, NJ, USA). Anti-human CD54-allophycocyanin (APC) antibody and its REA control-APC were from Miltenyi Biotec. (Bergisch Gladbach, German). Human C5a ELISA kit, fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD86 antibody and its isotype control were from BD Biosciences (San Diego, CA, USA). Gentamicin, ampicillin, 2-methyl-5-nitroimidazole-1-ethanol, and fradiomycin were from TCI Chemicals (Tokyo, Japan). Vancomycin was obtained from BBI Life Sciences Corporation (Shanghai, China). The mouse mast cell protease 1 (MMCP1) ELISA kit was from Invitrogen (San Diego, CA, USA). Protein G PLUS-Agarose was from Santa Cruz Biotechnology, Inc. (SantaCruz, CA, USA).

Xanthine (C₅H₄N₄O₂, ≥99%), Sodium borohydride (NaBH₄, ≥98%), Silver nitrate (AgNO₃, ≥99.8%), Polyvinylpyrrolidone (PVP) and Hydrogen peroxide (H₂O₂, 34.5–36.5%) were purchased from Sigma-Aldrich, Saint Louis, MO, USA. Sodium citrate dihydrate (C₆H₅Na₃O₇·2H₂O, ~99.8%) was purchased from J. T. Baker. L-Cysteine, L-Glutathione reduced, Threonine, Glutamine, L-PhenylAlanine, Leucine, L-Lysine, Tryptophan, Methionine, Glycine, Alanine, Aspartic acid, Albumins, Globulins, Fibrinogen Caffeine, Theophylline and Theobromine were obtained from Sigma-Aldrich. Sodium dihydrogen phosphate (NaH₂PO₄·H₂O, 99–102%) and Disodium hydrogen phosphate (Na₂HPO₄·2H₂O, 98–102%) were purchased from Merck.