Dissolution of the Panadol and Panadol Rapid was carried out in triplicate at 37.0 ± 0.5 °C using an USP II apparatus (Vision 8 Elite, Hanson Research, Chatsworth, CA, USA) and 500 mL FaSSIF pH 6.5. The paddle speed was set to 50 rpm and a syringe pump (Vision autoplus, Hanson Research, Chatsworth, CA, USA) was used to sample 1 mL (8.5 mL up-take volume) through in-line filtration (Millipore Millex-HV PVDF, 0.45 μm) at time points 5, 10, 15, 20, 30, 45, and 60 min without replacement. Samples were analyzed by HPLC-UV using a Waters Alliance 2690 system containing a quaternary solvent pump, automated sample manager, column oven, and UV detector (Waters 2489 module). The chromatographic separation was conducted on an Aeris Widepore XB-C8 (3.6 µm, 150 × 2.1 mm) column from Phenomenex (Torrance, CA, USA). The mobile phase was acetonitrile:50 mM potassium dihydrogen phosphate monobasic buffer (1:9). The column was maintained at 40 °C and the flow rate and injection volume were 0.4 mL/min and 3 μL, respectively. The UV detection was carried out at 215 nm and quantification was performed using the peak areas of a two-point linear standard curve at 1 mg/mL. The data were processed using Waters Empower 3 software.
Waters alliance 2690 system
Manufactured by Waters Corporation
Sourced in United States
The Waters Alliance 2690 system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative applications. The system features a modular design, allowing for customization and integration of various components to meet specific analytical requirements. The core function of the Alliance 2690 system is to separate, identify, and quantify components within a sample mixture through the process of liquid chromatography.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using waters alliance 2690 system
1
Dissolution Kinetics of Panadol and Panadol Rapid
2
Quantification of Plant Hormones in Pea
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The content of the hormones, abscisic acid (ABA), salicylic acid (SA), indoloacetic acid (IAA) and jasmonic acid (JA) were quantified in pea leaves and roots. Frozen powdered leaf tissues (400 mg) were extracted in ultrapure water (8 mL) according to Durgbanshi et al. (2005) . Before extraction, samples were spiked with deuterated standards of each hormone. Samples were centrifuged at 13,000 g for 10 min, the supernatant pH was adjusted with 30% acetic acid to 3.0 and partitioned twice against diethyl-ether. The organic layer was evaporated in a vacuum at
room temperature, and the dry pellet was resuspended with 1 mL of a water:methanol (9:1) solution. After filtration (0.22 µm cellulose acetate filter), 20 µL of the resulting solution was injected in an HPLC system (Waters Alliance 2690 system, Waters Corp., Milford, USA). Hormones were detected according to their specific transitions using a multiresidue mass spectrometric method (Quattro LC Triple Quadrupole, Micromass, Manchester, UK; for chromatographic and mass spectrometry details see Durgbanshi et al., 2005) .
room temperature, and the dry pellet was resuspended with 1 mL of a water:methanol (9:1) solution. After filtration (0.22 µm cellulose acetate filter), 20 µL of the resulting solution was injected in an HPLC system (Waters Alliance 2690 system, Waters Corp., Milford, USA). Hormones were detected according to their specific transitions using a multiresidue mass spectrometric method (Quattro LC Triple Quadrupole, Micromass, Manchester, UK; for chromatographic and mass spectrometry details see Durgbanshi et al., 2005) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!