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Aspartate transaminase ast

Manufactured by Merck Group
Sourced in Germany

Aspartate transaminase (AST) is a laboratory diagnostic enzyme assay that measures the activity of the enzyme aspartate transaminase in a biological sample. AST is an enzyme involved in amino acid metabolism and is present in various tissues of the body, including the liver, heart, and muscles. The AST assay is commonly used as a biomarker to assess liver function and detect liver damage or disease.

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2 protocols using aspartate transaminase ast

1

Antioxidant and Hepatoprotective Rutin Protocol

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Rutin (≥ 98%) was obtained from Sigma (Sigma-Aldrich GmbH, Sternheim, Germany) , and kept at 110℃ under 1.3 kPa for 12 h to remove bound water. Quercetin, BHA (butyl-hydroxyanisole) , BHT (butylhydroxytoluene) , TBHQ (tert.butylhydro quinine) , L-ascorbic acid, and linpalmitic acid were purchased from Merck. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH • ) , sodium phosphate buffer, tween 20 and absolute ethanol, Alanine transaminase (ALT) , Aspartate transaminase (AST) , Alkaline phosphates (ALP) , creatinine, and urea kites were purchased from Sigma (Sigma-Aldrich GmbH, Sternheim, Germany) . All other used chemicals were of analytical grade and were obtained from Merck and Sigma (Sigma-Aldrich GmbH, Sternheim, Germany) . Animals were obtained from the animal house -National research centre.
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2

Antioxidant and Hepatoprotective Potential

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Commercially tannic acid was purchased from El-Gomhoria Trade Pharmaceuticals & Chemicals Company, Cairo, Egypt. BHA (butyl-hydroxyanisole) , BHT (butylhydroxytoluene) , BHQ (tert.butylhydro quinine) , L-ascorbic acid and linoleic acid were purchased from Merck. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH ・ ) , sodium phosphate buffer, tween 20 and absolute ethanol, alanine transaminase (ALT) , aspartate transaminase (AST) , alkaline phosphates (ALP) , creatinine and urea kites were purchased from sigma (sigma-aldrich GmbH, sternheim, germany) . All other chemicals used were of analytical grade and were obtained from merck and sigma (sigma-aldrich GmbH, sternheim, germany) . Animals were obtained from animal house -National research centre.
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