Whole body fan beam software v8.269 3

These data were collected a few days prior to or on the morning of surgery. Dual-energy x-ray absorptiometry was performed with a Hologic QDR-4500A densitometer and the Whole-body fan beam software V8.269:3 (Hologic, Bedford, MA) was used to measure total body fat mass, fat percentage and lean body mass. Visceral and subcutaneous adipose tissue cross-sectional areas were measured by computed tomography with a GE Light Speed 1.1 CT scanner (General Electric Medical Systems, Milwaukee, WI). To perform the test, women had to lie in the supine position, their arms stretched above the head. To determine scanning position, a scout image of the body was used and images were obtained at the level of the L4-L5 vertebrae. To obtain visceral adipose tissue area, the intra-abdominal cavity was delineated using the ImageJ 1.33u software (National Institutes of Health, Bethesda, MD) at the internalmost aspect of the abdominal and oblique muscle walls around the cavity as well as the posterior aspect of the vertebral body. The area of subcutaneous adipose tissue was then calculated by subtraction of measured visceral adipose tissue area from total adipose tissue area at L4-L5. Highlighting and computation of adipose tissue areas were performed within the attenuation range of -190 to -30 Hounsfield units. All images were analyzed by the same observer.

Body fat mass, fat percentage and lean body mass values were obtained by DXA using a Hologic QDR-4500A densitometer and Whole-body fan beam software V8.269:3 (Hologic, Bedford, MA). Computed tomography (CT) was used as previously described [23] for subcutaneous and visceral adipose tissue cross-sectional area measurements with a GE Light Speed 1.1 CT scanner (GE Medical Systems, Milwaukee, WI). An attenuation range of -190 to -30 Hounsfield units was used for quantification of adipose tissue. Mean adipose tissue attenuation was also assessed as a marker of adipose tissue radiological density [3] .