Fluorescence-activated cell sorting (FACS) analysis was performed to examine the cell surface expression of TNF-R1 and TNF-R2, using fluorescein isothiocyanate (FITC)-conjugated anti-CD120a (TNF-R1) and anti-CD120b (TNF-R2) human monoclonal antibodies (MBL International, Woburn, MA). ARPE-19 cells were washed and trypsinized, then incubated with TNF-R1 and TNF-R2 antibodies according to the instructions from the manufacturer. Analysis was performed using a FACSCalibur flow cytometer and CellQuest software (BD Biosciences).
Fitc conjugated anti cd120a tnf r1 and anti cd120b tnf r2 human monoclonal antibodies
Manufactured by MBL Life Science
FITC)-conjugated anti-CD120a (TNF-R1) and anti-CD120b (TNF-R2) human monoclonal antibodies are laboratory reagents used for the detection and analysis of CD120a and CD120b, which are receptors for the proinflammatory cytokine tumor necrosis factor-alpha (TNF-α). These antibodies are labeled with the fluorescent dye FITC (fluorescein isothiocyanate), allowing them to be used in various immunological techniques, such as flow cytometry, to identify and quantify cells expressing these receptors.
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2 protocols using fitc conjugated anti cd120a tnf r1 and anti cd120b tnf r2 human monoclonal antibodies
1
FACS Analysis of TNF Receptor Expression
2
TNF-R1 and -R2 Expression Analysis
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We performed fluorescence-activated cell sorting (FACS) analysis to examine the cell surface expression of TNF-R1 and -R2, using fluorescein isothiocyanate (FITC)-conjugated anti-CD120a (TNF-R1) and anti-CD120b (TNF-R2) human monoclonal antibodies (mAbs; MBL International, Woburn, MA). FITC-conjugated mouse IgG1 was used as an isotype control (BD Biosciences, San Jose, CA). Experiments were performed using a FACS Canto II Flow Cytometer and FACS Diva software (BD Biosciences).
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