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Mda mb 453

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MDA-MB-453 is a cell line derived from a human breast adenocarcinoma. It is used as an in vitro model for breast cancer research.

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Lab products found in correlation

Mda mb 231, by American Type Culture Collection (336 mentions) Mcf 7, by American Type Culture Collection (267 mentions) Mda mb 468, by American Type Culture Collection (189 mentions) Skbr3, by American Type Culture Collection (181 mentions) Bt474, by American Type Culture Collection (156 mentions) Mcf 10a, by American Type Culture Collection (138 mentions) Bt549, by American Type Culture Collection (105 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (70 mentions) Hs578t, by American Type Culture Collection (63 mentions) Zr 75 1, by American Type Culture Collection (60 mentions) Hcc1937, by American Type Culture Collection (57 mentions) Hcc1954, by American Type Culture Collection (48 mentions) Bt 20, by American Type Culture Collection (45 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (44 mentions) Mda mb 436, by American Type Culture Collection (43 mentions) Mda mb 361, by American Type Culture Collection (41 mentions) Mda mb 453, by Thermo Fisher Scientific (38 mentions) Penicillin, by Thermo Fisher Scientific (37 mentions) Streptomycin, by Thermo Fisher Scientific (35 mentions) Mda mb 231, by Thermo Fisher Scientific (33 mentions)

468 protocols using mda mb 453

1

Breast Cancer Cell Lines Cultivation

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HER-2pos SKBR-3, triple negative MDA-MD-468, HER-2pos, trastuzumab resistant HCC1419, and HER-2pos, trastuzumab and lapatinib resistant MDA-MB-453 cell lines were obtained from American Type Culture Collection (ATCC; Rockwell, MA). MDA-MB-468, MDA-MB-453 and HCC1419 cell lines were re-authenticated by ATCC using STR prior to submission. Assays preformed using the SKBR-3 cell line were done within 6 months after the time of purchase from ATCC. Murine TUBO cells were a kind gift from Dr. Wei Zen Wei, (Wayne State University) and were derived from a neu transgenic mouse [45 (link)] and were cultured in RPMI medium. SKBR-3 cells were cultured in McCoy’s 5A Media (Corning, Manassas, VA), MDA-MB-468, MDA-MB-453, and HCC1419 cells were cultured in RPMI-1640 (ATCC). All media was supplemented with 10% v/v fetal calf serum (FBS; Atlanta Biologicals, Flowery Branch, GA), 100 units/ml of penicillin and 100 μg/ml of streptomycin sulfate (BioWhittaker, Walkersville, MD), 2 mmol/L glutamine (BioWhittaker), 1 mmol/L sodium pyruvate (BioWhittaker), and 1% non-essential amino acids (BioWhittaker). All cells were maintained in culture at 37°C in 5% CO2 in a humidified atmosphere.
Showalter L., Czerniecki B.J, & Koski G.K. (2020). Th1 cytokines in conjunction with pharmacological Akt inhibition potentiate apoptosis of breast cancer cells in vitro and suppress tumor growth in vivo. Oncotarget, 11(30), 2873-2888.
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2

Cancer Cell Culturing and Xenograft Processing

Cited 1 time
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MCF7, MDA-MB-453, A-431, H1781, H1819, Calu-3, BT-474, SKBR3, and NCI-N87 cell lines were purchased from the ATCC. MCF7, MDA-MB-453, and A-431 were cultured in DMEM; H1781, H1819, Calu-3, and NCI-N87 in RPMI-1640; BT-474 in ATCC HybriCare Medium with 1.5 g/L sodium bicarbonate; SKBR3 in the ATCC-formulated McCoy’s 5a Medium Modified. To make the complete medium, all media were supplemented with 10% FBS and 1% streptomycin/penicillin. All cells used in this study were periodically tested negative for Mycoplasma using the Mycoplasma Plus PCR Primer Kit (Agilent). All patient-derived cancer cells or tumor spheroids in this study were collected and studied according to the Declaration of Helsinki and DFCI IRBs. DFCI 429 was established from a pleural effusion sample. Next-Generation Sequencing confirmed de novo HER2 amplification. DFCI315 and DFCI359 were characterized to harbor HER2 exon20 V777_G778insGSP and HER2 exon19 755_757LREdelinsRP, respectively (23 (link)). Xenograft tumors were processed for short-term ex vivo as previously described (23 (link)). The spheroids in a range of 40 to 70 μmol/L were resuspended in type I collagen (Corning) and loaded into the DAX-1 3-D cell culture chip (AIM Biotech). After solidification, the media were applied to the outer channels with indicated drugs.
Inoue M., Kobayashi M., Kozaki S., Zimmer A, & Ueda H. (1998). Nociceptin/orphanin FQ-induced nociceptive responses through substance P release from peripheral nerve endings in mice. Proceedings of the National Academy of Sciences of the United States of America, 95(18), 10949-10953.
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3

Cultivation of Mammary Cell Lines

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Normal human mammary epithelial cell line (MCF-10A) and human BC cell lines (MDA-MB-453, MDA-MB-231, MCF-7, and MDA-468) were attained from ATCC (Manassas, VA, USA). MCF-10A cells were kept in DMEM/F12 (D9785, Sigma-Aldrich, St. Louis, MO, USA) with the supplementation of 5% HS (23491-45-4, Sigma-Aldrich), 20 ng/mL epidermal growth factor (EGF; PHG0311, Thermo Fisher Scientific, Rockford, IL, USA), 0.5 μg/mL hydrocortisone (211H-500, Sigma-Aldrich), 1% NEAA (M7145, Sigma-Aldrich), 10 μg/mL insulin (12,643, Sigma-Aldrich) and 1% P/S (PB180120, Procell, Wuhan, China). MCF-7 cells were cultivated in DMEM (D9785, Sigma-Aldrich) with 10% fetal bovine serum (FBS; Gibco, Rockville, MD, USA), 10 µg/mL human insulin and 1 µM 4-hydroxytamoxifen (SML1666, Sigma-Aldrich). MDA-MB-231, MDA-468 and MDA-MB-453 cells were cultivated in ATCC-formulated Leibovitz’s L-15 Medium (11,415,049, Thermo Fisher Scientific) with 10% FBS. All the cells were maintained at 37 °C with 5% CO2. The STR reports were provided in Additional file 5.
An C., Hu Z., Li Y., Zhao P., Liu R., Zhang Q., Zhu P., Li Y, & Wang Y. (2022). LINC00662 enhances cell progression and stemness in breast cancer by MiR-144-3p/SOX2 axis. Cancer Cell International, 22, 184.
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4

Culturing Breast Cancer Cell Lines

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A normal human breast epithelial cell line (MCF-10A) and BC cell lines (SK-BR-3, BT549, MCF7, MDA-MB-231, MDA-MB-453, and BT20) were purchased from American Tissue Culture Collection (ATCC, Manassas, USA). MCF-10A cells were cultured using the MEGM Bullet Kit (CC-3150; Lonza/Clonetics Corporation, Basel, Switzerland). SK-BR-3 cells were cultured in McCoy's 5A Modified Medium (30-2007; ATCC). BT549 cells were cultured in Roswell Park Memorial Institute Medium 1640 (10491; Solarbio, Beijing, China). MCF7 cells were cultured in Eagle's Minimum Essential Medium (30-2003; ATCC). MDA-MB-231 and MDA-MB-453 cells were cultured in Leibovitz's L-15 Medium (30-2008; ATCC). BT20 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM, 11965; Solarbio). All culture media were supplemented with 10% fetal bovine serum (FBS, 11011-8611; Solarbio) and 1% penicillin-streptomycin liquid (P1400; Solarbio).
Li Y., Zeng Q., Qiu J., Pang T., Ye F., Huang L, & Zhang X. (2020). MiR-183-5p Promotes Proliferation, Metastasis and Angiogenesis in Breast Cancer Cells through Negatively Regulating Four and a Half LIM Protein 1. Journal of Breast Cancer, 23(4), 355-372.
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5

Evaluating Vitamin C and PI3K Inhibitors on TNBC Cell Lines

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TNBC cell lines including BT20 and MDA-MB-453, which both carry activating PIK3CA mutations, were purchased from ATCC (Manassas, VA) in the year of 2018 without further authentication. Frozen cells were newly thawed from low (2-5) passages. Testing for mycoplasma was performed using PlasmoTest Mycoplasma detection kits (Thermo Fisher, Waltham, MA) with only mycoplasma negative cells included in all experiments. BT20 cells were maintained under a 5% CO2 atmosphere in Eagle's Minimum Essential Medium (EMEM), while MDA-MB-453 cells were cultured in Leibovitz's L-15 Medium (ATCC), supplemented with 10% heat-inactivated fetal bovine serum, 100 Units/ ml penicillin, and 100 μg/ml of streptomycin. TNBC cells were seeded for 24 hours and subsequently treated with vitamin C (sodium ascorbate, Sigma-Aldrich, St. Louis, MO) and different PI3K inhibitors at various concentrations. Media was changed daily to ensure the presence of fresh vitamin C.
Mustafi S., Camarena V., Qureshi R., Sant D.W., Wilkes Z., Bilbao D., Slingerland J., Kesmodel S.B, & Wang G. (2021). Vitamin C sensitizes triple negative breast cancer to PI3K inhibition therapy. Theranostics, 11(8), 3552-3564.
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6

Culturing Human Breast Cancer Cell Lines

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The human breast carcinoma cell lines (BT-549, MDA-MB-231, BT-474, EFM-192A, MDA-MB-453, SK-BR-3, and ZR-75-1) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in RPMI, except for MDA-MB-453 and SK-BR-3 in DMEM. The media were added with 10% of FBS, 1% of penicillin, 1% of streptomycin, and 1% of L-glutamine. Approximately 20% of FBS was used for EFM-192A and BT-474 cells. Medium for BT-474 was supplemented with 10 μg/mL of insulin. All reagents, unless specified, are from SigmaAldrich (St. Louis, MO, USA). HGF (recombinant human hepatocyte growth factor NS0-expressed) was purchased from R&D systems (Minneapolis, MN, USA). MET tyrosine kinase inhibitor JNJ-38877605 (JNJ) was purchased from Selleckchem (Houston, TX, USA).
Gallo S., Vitacolonna A., Comoglio P, & Crepaldi T. (2022). MET Oncogene Controls Invasive Growth by Coupling with NMDA Receptor. Cancers, 14(18), 4408.
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7

Breast Cancer Cell Line Maintenance

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MDA-MB-231, BT-20, HCC1937 breast cancer cells and HMEC were maintained and grown as previously described [14 (link), 15 (link), 31 (link)]. MDA-MB-453, MDA-MB-157, Hs578T were purchased in the year 2015 from ATCC and grown according to the ATCC recommended culture conditions. All cell lines were authenticated by genomic STR profile.
Singha P.K., Pandeswara S., Geng H., Lan R., Venkatachalam M.A., Dobi A., Srivastava S, & Saikumar P. (2019). Increased Smad3 and reduced Smad2 levels mediate the functional switch of TGF-β from growth suppressor to growth and metastasis promoter through TMEPAI/PMEPA1 in triple negative breast cancer. Genes & Cancer, 10(5-6), 134-149.
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8

Cell Line Maintenance and Reagents

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AU565, HCC1419, NCI-H2170, HCC202, HCC1954, NCI-N87, ZR75-1, SKOV3, ZR75-30, MDAMB175VII, CALU3, MDAMB453, MDAMB361, JIMT1, SKBR3 and HCC2218 cells were obtained from ATCC. OE19 and OE33 were obtained from ECCC; COLO-678 was obtained from DSMZ, and KYSE-410 from Sigma-Aldrich. BT-474-M3 cells (hereafter simply referred to as BT-474) were obtained from Hermes biosciences. All cell lines were maintained in RPMI supplemented with 10% FBS, penicillin, and streptomycin. GSK-1120212 and MK-2206 were purchased from Selleckchem. Recombinant human HRG-β1 (EGF domain) was from R&D Systems.
Kirouac D.C., Du J., Lahdenranta J., Onsum M.D., Nielsen U.B., Schoeberl B, & McDonagh C.F. (2016). HER2+ Cancer Cell Dependence on PI3K vs. MAPK Signaling Axes Is Determined by Expression of EGFR, ERBB3 and CDKN1B. PLoS Computational Biology, 12(4), e1004827.
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9

Metastatic Breast Cancer Cell Lines

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MCF-7, T-47D, BT-474, MDA-MB-453, Hs578T and BT-549 cells were purchased from ATCC (Manassas, VA). MDA-MB-231 cells were obtained from Dr. Suyun Huang (MD Anderson Cancer). MDA-MB-231-LM2 (LM2) cells were provided by Dr. Joan Massagué Lab (Memorial Sloan-Kettering Cancer Center). The LM2 cells, a derivative of MDA-MB-231 cells, were selected for its strong ability to metastasize to the lung.21 (link) All above cells were cultured following instructions from ATCC. SUM-159 cells were obtained from Dr. Stephen Ethier (Wayne State University) and cultured in F-12 supplemented with 5% FBS, 1% penicillin/streptomycin at 37 °C in a humidified 5% CO2 atmosphere.
Wang Z., Li Y., Xiao Y., Lin H.P., Yang P., Humphries B., Gao T, & Yang C. (2019). Integrin α9 depletion promotes β-catenin degradation to suppress triple negative breast cancer tumor growth and metastasis. International journal of cancer, 145(10), 2767-2780.
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Culturing Common Cancer Cell Lines

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The HEPG2, MCF7, BT474, MDA.MB.231, MDA.MB.468, MDA.MB.453, and MDA.MB.157 cell lines were originally obtained from ATCC (Manassas, USA). Cell lines were routinely cultured in Dulbecco's Modified Eagle Medium (DMEM; Thermo Fisher, UK, Cat. 31966047), supplemented with 10% Fetal Bovine Serum (FBS; Thermo Fisher, UK, Cat. 11560636) and incubated in 37°C, 5% CO2. Cells were periodically checked and confirmed to be mycoplasma free.
Lianto P., Hutchinson S.A., Moore J.B., Hughes T.A, & Thorne J.L. (2021). Characterization and prognostic value of LXR splice variants in triple-negative breast cancer. iScience, 24(10), 103212.
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