Beas 2b cell line

The BEAS-2B cell line was purchased from the Chinese Academy of Sciences Cell Bank (CASCB, Shanghai, China) and cultured in BEGM media (Lonza, Walkersville, MD, USA, CC-3170). Lung cancer cell lines, including SPC-A1, H358, A549, and H1299, were purchased from the Chinese Academy of Sciences Cell Bank (CASCB, China) with STR documents and were cultured in RPMI-1640 medium (Corning, Tewksbury, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. The siRNA for SGO1 and NC were transfected into cells. Transfection was performed using the Lipofectamine 3000 transfection reagent based on the manufacturer’s protocol. SGO1 siRNA: ACATGCACATACCTGAAAATG.

The BEAS-2B cell line was purchased from the Chinese Academy of Sciences Cell Bank (CASCB, China) and cultured in Bronchial Epithelial Cell Growth Medium (Lonza, CC-3170). The lung cancer cell lines, including HCC827, H1650, A549, and H1975, were purchased from the CASCB (China) with STR documents and were cultured in RPMI-1640 medium (Corning) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin.

The BEAS-2B cell line was purchased from the Chinese Academy of Sciences Cell Bank (CASCB, China), and cultured in BEGM media (Lonza, CC-3170). Lung cancer cell lines, including H358, H1650, A549, and H1299, were purchased from CASCB with STR documents, and were cultured in RPMI-1640 medium (Corning) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin.