Syringe filters

Manufactured by Iwaki

Sourced in Japan

Syringe filters are a type of laboratory equipment used to remove particulates, microorganisms, or other contaminants from liquid samples. They are designed to be attached directly to a syringe and function by passing the liquid through a porous membrane, which traps the unwanted materials.

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Lab products found in correlation

4 protocols using syringe filters

Preparing Pharmacological Reagents

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Melphalan, echinomycin, U0126, LY294002, and dimethyl fumarate (DMF) were purchased form Wako (Tokyo, Japan). These reagents were dissolved in dimethyl sulfoxide. These reagents were diluted in PBS (0.05 M, pH7.4), filtrated through syringe filters (0.45 μm, IWAKI GLASS, Tokyo, Japan) and used for various assays described below.

Tsubaki M., Takeda T., Tomonari Y., Koumoto Y.I., Imano M., Satou T, & Nishida S. (2019). Overexpression of HIF-1α contributes to melphalan resistance in multiple myeloma cells by activation of ERK1/2, Akt, and NF-κB. Laboratory investigation; a journal of technical methods and pathology, 99(1).

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Lipid Biosynthesis Inhibitor Screening

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Fluvastatin, simvastatin, and MG132 were purchased from Calbiochem (San Diego, CA, USA). Mevalonic acid lactone (MVA), FPP, GGPP, squalene, ubiquinone, isopentenyladenine, and dolichol were purchased from Sigma (St. Louis, MO, USA). U0126 was purchased from Promega (Madison, WI, USA). Rapamycin, z-VAD-fmk, z-LEHDfmk, and z-DEVE-fmk were purchased form Wako (Osaka, Japan). These reagents were dissolved in dimethyl sulfoxide. And then, these dissolved reagents were resuspended in phosphate-buffered saline (PBS; 0.05 M, pH 7.4) and filtrated through syringe filters (0.45 μm; IWAKI GLASS, Tokyo, Japan) before use.

Fujiwara D., Tsubaki M., Takeda T., Tomonari Y., Koumoto Y.I., Sakaguchi K, & Nishida S. (2017). Statins induce apoptosis through inhibition of Ras signaling pathways and enhancement of Bim and p27 expression in human hematopoietic tumor cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 39(10).

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Kinase Inhibitor Assay Protocol

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Imatinib, nilotinib, dasatinib, bafetinib, ponatinib, DCC-2036, GNF-2, GNF-5, EPZ005687, XAV-939, and FH535 were purchased from Selleckchem (Houston, TX, USA). PHA665752 was purchased from MedChemExpress, LLC (Princeton, NJ, USA). U0126, LY294002, and SP600125 were purchased form Wako (Tokyo, Japan). These reagents were dissolved in dimethyl sulfoxide and diluted in phosphate-buffed saline (PBS; 0.05 M, pH 7.4), filtrated through syringe filters (0.45 μm, IWAKI GLASS, Tokyo, Japan), and used for various assays described below.

Tsubaki M., Takeda T., Kino T., Sakai K., Itoh T., Imano M., Nakayama T., Nishio K., Satou T, & Nishida S. (2017). Contributions of MET activation to BCR-ABL1 tyrosine kinase inhibitor resistance in chronic myeloid leukemia cells. Oncotarget, 8(24), 38717-38730.

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Bisphosphonate and Isoprenoid Compound Preparation

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Minodronate was supplied from Astellas Pharmaceutical (Tokyo, Japan). Alendronate was purchased from LKT laboratories Inc. (St. Paul, MN, USA). These reagents were dissolved in phosphate-buffered saline (PBS; 0.05 M, pH7.4), filtrated through Syringe Filters (0.45 μm; IWAKI GLASS, Tokyo, Japan), and used for the various assays described below.
Farnesol (FOH) and geranylgeraniol (GGOH) were purchased from Sigma (St Louis, MO, USA). FOH and GGOH were dissolved in dry ethanol. U0126 and LY294002 were purchased from Promega (Southampton, Hants, UK) and dissolved in DMSO. The dissolved reagents were resuspended in PBS (0.05 M, pH 7.4) and filtered through Syringe Filters before use.

Tsubaki M., Komai M., Itoh T., Imano M., Sakamoto K., Shimaoka H., Takeda T., Ogawa N., Mashimo K., Fujiwara D., Mukai J., Sakaguchi K., Satou T, & Nishida S. (2014). Nitrogen-containing bisphosphonates inhibit RANKL- and M-CSF-induced osteoclast formation through the inhibition of ERK1/2 and Akt activation. Journal of Biomedical Science, 21(1), 10.

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