Nitric oxide tank

The following chemicals and materials were used as received: 0.5 mm gold wire, 0. 5 mm silver wire, firefly lantern extract, catechol, dopamine hydrochloride, chloroplatinic acid hydrate, lead (II) acetate trihydrate, Hanks’ balanced salt solution (HBSS), TES (Trizma acetate, ethylenediaminetetraacetic acid (EDTA), sucrose) buffer, and potassium nitrate (Sigma Aldrich, St. Louis, MO); Armstrong C-7 resin, Activator A and E (Ellsworth Adhesives, Germantown, WI); silver conductive epoxy (MG Chemicals, Burlington, ON, Canada); J-B Kwik (J-B Weld Co., Sulphur Springs, TX); 250 and 500 μm platinum wire, 2 mm palladium wire, and 1 mm glassy carbon rod (Alfa Aesar, Ward Hill, MA); soldering wire and heat shrink tubes (Radio Shack); isopropanol and acetone (Fisher Scientific, Springfield, NJ); colloidal silver (Ted Pella, Redding, CA); electrode polishing pads (CH Instruments, Austin, TX; Allied High Tech Products, Inc., Rancho Dominguez, CA); nitric oxide (NO) tank (99.5%) (Airgas Inc., Radnor, PA); polyetheretherketone (PEEK) fitting nuts (P-131: 1/8” outer diameter (o.d.) tubing, P-137: 3/16” o.d. tubing), one-piece finger tight fittings for 1/16” o.d. tubing (IDEX Health & Science, Oak Harbor, WA); Nafion (5% w/w Nafion, Ion Power Inc., New Castle, DE or Sigma Aldrich, St. Louis, MO).

The following chemicals and materials were used as received: Nano SU-8 developer, SU-8 50 (Microchem, Newton, MA, USA); catechol, potassium dicyanoaurate (I), sodium carbonate, sodium phosphate monobasic, chloroplatinic acid hydrate, lead (II) acetate trihydrate, Hanks balanced salt solution (HBSS), sodium dodecyl sulfate, and potassium nitrate (Sigma Aldrich, St. Louis, MO, USA); 1 mm glassy carbon rod (Alfa Aesar, Ward Hill, MA, USA); Au, 3 mm 200 mesh regular grid (SPI supplies, West Chaster, PA, USA); soldering wire, extending wire and heat shrink tubes (RadioShack); 70 mL disposable aluminum dish, isopropanol and acetone (Fisher Scientific, Springfield, NJ, USA); colloidal silver (Ted Pella, Redding, CA, USA); electrode polishing pads (Allied High Tech Products, Inc., Rancho Dominguez, CA, USA); Permatex 5 minute epoxy (ITW Performance Polymers, IL, USA); Nitric oxide (NO) tank (99.5%, Airgas Inc., Radnor, PA, USA); polystyrene (PS) powder (Goodfellow Cambridge Ltd., Huntingdon, England).