NR4A1 antibody was purchased from Abcam (Cambridge, MA), and TXNDC5, PGK1 and ATP5A1 antibodies were purchased from GeneTex (Irvine, CA). XBP-1s (spliced XBP-1) and phosphor-PERK (Ser713) antibodies were purchased from BioLengend (San Diego, CA, USA). ATF4, CHOP, GRP78, β-actin, and IDH1 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and ATF6 andibody was purchased from Abgent (Atlanta, GA, USA). All remaining antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). DIM-C-pPhOH was synthesized and purified in this laboratory as previously described (19 (link)). Reporter lysis buffer, luciferase reagent, and β-galactosidase (β-gal) reagent were supplied by Promega (Madison, WI, USA). Quantitative real-time PCR and western blot analysis were undertaken as previously described (20 (link)). The real-time PCR primers for NR4A1 were purchased from Qiagen (Valencia, CA, USA), and all other sequences of the primers used for real-time PCR were shown in Supplemental Table 1 .
β galactosidase β gal reagent
Manufactured by Promega
Sourced in United States
The β-galactosidase (β-gal) reagent is a laboratory tool used to detect and measure the presence of the β-galactosidase enzyme. The reagent is designed to facilitate the visualization and quantification of β-galactosidase activity in various experimental systems.
Automatically generated - may contain errors
2 protocols using β galactosidase β gal reagent
1
Protein Expression Analysis in Cells
2
Antibody Sourcing and Gene Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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NR4A1 and thioredoxin domain-containing 5 (TXNDC5) antibodies were purchased from Abcam (Cambridge, MA, USA) and GeneTex (Irvine, CA, USA), respectively. Activating transcription factor 4 (ATF4) and glucose regulatory protein 78 (GRP78) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and all remaining antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Reporter lysis buffer, luciferase reagent, and β-galactosidase (β-Gal) reagent were obtained from Promega (Madison, WI, USA). Quantitative real-time PCR and Western blot analysis were undertaken as previously described [3 (link)]. The sequences of the primers used for real-time PCR were as follows: survivin sense 5′-CAG ATT TGA ATC GCG GGA CCC-3′, antisense 5′-CCA AGT CTG GCT CGT TCT CAG-3′ and TBP sense 5′-TGC ACA GGA GCC AAG AGT GAA-3′, antisense 5′-CAC ATC ACA GCT CCC CAC CA-3′.
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