Shimadzu ICP 9000 (with Mini plasma Torch in axial reading mode) was used to measure the zinc content. Standard solution for AAS (Sigma Aldrich) was used to generate the calibration curve between 10 to 500 µg/L in pure water with 1% of HNO3 (Fluka). Samples were routinely incubated in HNO3 10% ON at RT. Briefly, 500 µl of each cytosolic crude extract was incubated with 90 µl of HNO3 65% ON at RT. The sample was centrifuged at 13,000 rpm for 5 min. Before to be measured pure water was added to the supernatant extemporaneously to obtain a final volume of 7 ml. Ytterbium solution standard for AAS (Sigma Aldrich) was used as an internal standard to prevent calibration drift and fluidic perturbation. The result was express in µg/L [Zn] per mg of protein.
Ytterbium solution standard for aas
Manufactured by Merck Group
Ytterbium solution standard for AAS is a laboratory product that provides a reference solution for the calibration and verification of atomic absorption spectrometry (AAS) equipment. It contains a known concentration of the element ytterbium, which is used to ensure the accuracy and precision of AAS measurements.
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Lab products found in correlation
2 protocols using ytterbium solution standard for aas
1
Zinc Content Quantification in Cellular Extracts
2
Quantifying Zinc in Cytosolic Extracts
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Shimadzu ICP 9000 (with Mini plasma Torch in axial reading mode) was used to measure the zinc content. Standard solution for AAS (Sigma Aldrich) was used to generate the calibration curve between 10 to 500 µg/L in pure water with 1% of HNO3 (Fluka). Samples were routinely incubated in HNO3 10% ON at RT. Briefly, 500µl of each cytosolic crude extract was incubated with 90µl of HNO3 65% ON at RT. The sample was centrifuged at 13,000 rpm for 5 min. Before to be measured pure water was added to the supernatant extemporaneously to obtain a final volume of 7 ml. Ytterbium solution standard for AAS (Sigma Aldrich) was used as an internal standard to prevent calibration drift and fluidic perturbation. The result was express in µg/L [Zn] per mg of protein.
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