Cftm594 tunel apoptosis detection kit

Male adult C57BL/6 mice (8-9 weeks old), from Charles River Laboratories, were maintained and experimental procedures were conducted according to NIH guidelines and approved by the University of Minnesota Institutional Animal Care and Use Committee (Protocol no. 1007A86332). Lentivirus carrying RIP140-shRNA, was delivered to hippocampus using stereotaxic apparatus at anteroposterior (AP) 2.0 mm, medial-lateral (ML) 1.2 mm and dorsoventral (DV)1.6 mm according to previous describe⁶⁵ . RIP140 protein levels following virus injection were detected using immunofluorescence and western blot as indicated in the figure legend. The ER stress animal models were generated following intraperitoneal injection of Tg(1 μg/g) or normal saline(control) after 3 or 18 days of virus injection. Then the animals were anesthetized 2 days later and brain tissues were subjected to immunofluorescence staining or protein extraction. To detect the apoptotic cells, TUNEL staining of brain sections was performed using CF^TM594 TUNEL apoptosis detection kit (30064, Biotium). Images were acquired by Olympus FluoView 1000 IX2 upright confocal microscope. The TUNEL-positive cell numnber versus total cell number indicated by DAPI from the Lentivirus-infected areas was counted and quantified. Background was adjusted using Image J before counting.