Integra dermal regeneration template

HDFs were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) FBS (Gibco, Thermo Fisher Scientific) and 1% (v/v) antibiotic-antimycotic solution, and incubated at 37 °C in a 5% CO₂ atmosphere incubator. Media was changed every 48 h. For sterilization of the scaffolds, 8 mm biopsies of PG2 and the Integra® dermal regeneration template (Integra Life Sciences, Plainsboro, NJ, USA, abbreviated as Control hereafter) were placed in a 48-well plate and sterilized by ultra violet light for 30 min. The UV sterilization was performed inside a biosafety cabinet equipped with a germicidal UV lamp of 255–280 nm. The distance between the UV lamp and the object was the distance between the lamp and the floor of the cabinet ( 100 cm). Sterile PBS solution was added into the plate to pre-dissolve the scaffolds 24 h before seeding. Prior to seeding, fibroblasts within a passage of 10 were trypsinized and re-suspended in the medium at a concentration of 2 × 10⁵ per mL. A cell suspension (10 μL) was added onto scaffolds placed in a 48-well plate. Facilitate the cell uptake into the interior of the scaffold for 1 h in the incubator and then add 500 μL medium to each well. The medium was changed every 48 h.

Burn eschar was excised as early as possible after completion of resuscitation, and sites planned for treatment with ESS were covered with cadaveric allograft, or the dermal replacement, Integra® Dermal Regeneration Template (Integra LifeSciences Corp; Plainsboro, NJ)^{28 (link),42 (link)}. Grafting and post-operative care were performed as described previously^{31 (link),43 (link),44 (link)}.