1 ethyl 3 3 dimethylamino propyl carbodiimide hcl edc

Quartz substrate (JGS1) was purchased from Wuxi Crystal and Optical Instrument Co. Ltd., China. Sulfuric acid, hydrogen peroxide, and sucrose were purchased from Sinopharm Chemical Reagent Beijing, Co. Ltd., China. Deionized (DI) water was purchased from Solarbio Beijing, Co. Ltd., China. Ethanol was purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd., China. Polydimethylsiloxane (PDMS) was obtained from Dow Corning, USA. Ethanolamine, thiolated alkane 10-carboxy-1-decanethiol (11-MUA), 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide-HCl (EDC), N-hydroxy succinimide (NHS), and bovine serum albumin (BSA) were purchased from Sigma–Aldrich, USA. Phosphate buffer solution (PBS: 0.0067 M, pH: 7.4) was purchased from Thermo Fisher, USA. Cancer antigen 125 protein (CA125, MUC16-1H) and cancer antigen 15-3 (CA15-3, MUC1-376H) were purchased from Creative BioMart, USA. Anti-MUC16 antibody (for CA125, ab134093) and anti-MUC1 antibody (for CA15-3, ab109185) were purchased from Abcam, USA. More information about materials, chemicals, and biologicals was listed in Table S1.

Crizotinib (Shanghai Haoyuan Chemexpress Co., Ltd. Shanghai, China) used as received; its purity was > 99%. Bovine serum albumin (BSA), 3,3`,5,5`-tetramethyl-benzidine, peroxidase substrate solution (TMB), N-hydroxysuccinamide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl carbodiimide HCl (EDC) were purchased from Sigma Chemicals Co. (St. Louis, USA). Keyhole limpet hemocyanin (KLH) was purchased from Novabiochem Co. (La Jolla, USA). Freund’s adjuvants (complete and incomplete), dialysis tubes cell membrane, and goat anti-mouse IgG-horseradish peroxidase conjugate (HRP-IgG, catalogue number: A5278) were obtained from Sigma-Aldrich (St. Louis, USA). BCA protein assay kit was a product of Pierce Chemical Co. (Rockford, USA). EIA/RIA high-binding flat bottom polystyrene 96-microwell plates) were obtained from Corning/Costar Inc., Corning (New York, USA). Waters C18 analytical HPLC column (150 mm × 4.6 mm, 5 μm) manufactured by Altmann Analytik GmbH & Co. (Deutschland, Germany). All other chemicals and solvents used throughout the work were of analytical grade.