Pe cy7 sca

Manufactured by BD

PE-Cy7-Sca is a fluorescent label that can be used to detect and analyze cell surface markers in flow cytometry applications. It is a combination of the phycoerythrin (PE) fluorophore and the cyanine-7 (Cy7) dye, which together provide a bright and stable signal for flow cytometric analysis.

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Lab products found in correlation

C kit apc, by BD (4 mentions) Apc cy7 cd48, by Thermo Fisher Scientific (4 mentions) Lineage cell depletion kit, by Miltenyi Biotec (3 mentions) Pacific blue cd150, by BioLegend (3 mentions) Hepes, by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Merck Group (3 mentions) Streptavidin pe secondary antibody, by BD (2 mentions) Facsaria cell sorter, by BD (2 mentions) Fxcycle pi rnase staining solution, by Thermo Fisher Scientific (1 mentions) Cytoflex 5 flow cytometer, by Beckman Coulter (1 mentions) Ki 67 pe cy7, by BioLegend (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by BioLegend (1 mentions)

Close spelling variants of this product

Sca-1-PE-Cy7 (D7, PE-Cy7-conjugated anti-SCA-1 (clone D7, PE-Cy7-conjugated anti-Sca-1 antibodies Sca-1-PE-Cy7 Sca-1-PE-Cy7 (clone D7;

4 protocols using pe cy7 sca

Isolation of Murine Hematopoietic Stem Cells

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Bone marrow cells were harvested from tibia and femurs of mice by gentle flushing with HBSS++ buffer [Hanks balanced salt solution (10–547F, Lonza) + HEPES (BP299–100, Fisher Scientific) + Fetal bovine serum (F2442, Sigma) + penicillin-streptomycin (15140–122, GIBCO)]. Samples were filtered through a 70 μM filter to obtain a single cell suspension and Lin⁻ enrichment was performed with lineage negative selection using the lineage cell depletion kit (130–090-858, Miltenyi). For isolation of LSK cells and LT-HSCs, Lin⁻ cells were incubated with biotin-labeled lineage antibody cocktail from BD containing a mixture of antibodies against CD3, CD11b, CD19, B220, Gr-1 and Ter119 (51–09082J, BD Pharmingen), followed by staining with streptavidin-PE secondary antibody (554061, BD). LSK cells were identified as Lin-Sca-1+c-kit+ using PE-Cy7-Sca (Clone D7, 558162, BD Biosciences) and APC-c-kit (Clone ACK2, 135108, BD Biosciences) antibodies; whereas LT-HSCs were recognized by being LSK and CD150+CD48- using PE-Cy7-Sca (Clone D7, 558162, BD Biosciences), APC-c-kit (Clone ACK2, 135108, BD Biosciences), Pacific Blue-CD150 (Clone TC15–12F12.2, 115924, Biolegend) and APC-Cy7-CD48 (Clone HM48–1, 47–0481-82, e-Bioscience). Cells were sorted using a BD FACSAria cell sorter.

Rodríguez A., Yang C., Furutani E., de Teresa B.G., Velazquez M., Filiatrault J., Sambel L.A., Phan T., Flores-Guzmán P., Sánchez S., Orozco A.M., Mayani H., Bolukbasi O.V., Färkkilä A., Epperly M., Greenberger J., Shimamura A., Frías S., Grompe M., Parmar K, & D’Andrea A.D. (2020). Inhibition of TGFβ1 and TGFβ3 promotes hematopoiesis in Fanconi anemia. Experimental hematology, 93, 70-84.e4.

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Isolation of Long-Term Hematopoietic Stem Cells

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Mice were sacrificed and the bone marrow from tibia and femurs was harvested by gentle flushing with HBSS++ buffer [Hanks balanced salt solution (10-547F, Lonza) + HEPES (BP299-100, Fisher Scientific) + Fetal bovine serum (F2442, Sigma) + penicillin-streptomycin (15140–122, GIBCO)]. A 70 μM filter was used for filtering the samples and obtaining a single cell suspension. Lin^- enrichment was performed with lineage negative selection using the lineage cell depletion kit (130-090-858, Miltenyi).
For isolation of LT-HSCs, identified as Lin^-Sca-1⁺c-kit⁺ CD150⁺CD48^-, Lin^- cells were incubated with a mixture of biotin-labeled lineage antibody cocktail against CD3, CD11b, CD19, B220, Gr-1 and Ter119 (51-09082J, BD Pharmingen), and fluorochrome conjugate antibodies PE-Cy7-Sca (Clone D7, 558162, BD Biosciences), APC-c-kit (Clone ACK2, 135108, BD Biosciences), Pacific Blue-CD150 (Clone TC15-12F12.2, 115924, Biolegend) and APC-Cy7-CD48 (Clone HM48-1, 47-0481-82, e-Bioscience), followed by incubation with streptavidin-PE secondary antibody (554061, BD). LT-HSCs were sorted with a BD FACSAria cell sorter.

Rodríguez A., Epperly M., Filiatrault J., Velázquez M., Yang C., McQueen K., Sambel L.A., Nguyen H., Iyer D.R., Juárez U., Ayala-Zambrano C., Martignetti D.B., Frías S., Fisher R., Parmar K., Greenberger J.S, & D’Andrea A.D. (2022). TGFβ pathway is required for viable gestation of Fanconi anemia embryos. PLOS Genetics, 18(11), e1010459.

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Cell Cycle Analysis in Lymphoblast and HSC

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Cell cycle analysis in lymphoblast cell lines were thoroughly resuspended and fixed with ice-cold 70% ethanol and stored at −20 during 30 min. After fixation, cells were washed with PBS, stained with FxCycle PI/RNase staining solution (Invitrogen, F10797) and analyzed in a CytoFlex 5 Flow Cytometer (Beckman Coulter). Cell cycle analysis in mouse LT-HSC was performed using the cell surface markers PE-Cy7-Sca (Clone D7, 558162, BD Biosciences), APC-c-kit (Clone ACK2, 135108, BD Biosciences), FITC-CD150 (Clone A12 (7D4), 306306, Biolegend) and APC-Cy7-CD48 (Clone HM48-1, 470481-82, e-Bioscience), along with intracellular staining using PE-Cy7-Ki67 (350526, Biolegend) and DAPI (422801, Biolegend). Data analysis was performed using FlowJo software version 10.5.3.

Rodríguez A., Zhang K., Färkkilä A., Filiatrault J., Yang C., Velázquez M., Furutani E., Goldman D.C., de Teresa B.G., Garza-Mayen G., McQueen K., Sambel L.A., Molina B., Torres L., González M., Vadillo E., Pelayo R., Fleming W.H., Grompe M., Shimamura A., Hautaniemi S., Greenberger J., Frías S., Parmar K, & D’Andrea A.D. (2020). MYC Promotes Bone Marrow Stem Cell Dysfunction in Fanconi Anemia. Cell stem cell, 28(1), 33-47.e8.

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Isolation and Identification of Mouse LT-HSCs

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BM cells were harvested from tibia and femurs of mice by gentle flushing with HBSS++ buffer [Hanks balanced salt solution (10-547F, Lonza) + HEPES (BP299-100, Fisher Scientific) + Fetal bovine serum (F2442, Sigma) + penicillin-streptomycin (15140-122, GIBCO)]. Samples were filtered through a 70 μM filter and Lin- enrichment was performed using the lineage cell depletion kit (130-090-858, Miltenyi). LT-HSCs were recognized by being LSK (Lin-Sca-1+c-Kit+) and CD150+CD48- using PE-Cy7-Sca (Clone D7, 558162, BD Biosciences), APC-c-kit (Clone ACK2, 135108, BD Biosciences), Pacific Blue-CD150 (Clone TC15-12F12.2, 115924, Biolegend) and APC-Cy7-CD48 (Clone HM48-1, 47-0481-82, e-Bioscience).

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