Human p53 clone do 1

Recombinant Tat protein from HIV-1 (subtype B) was expressed, purified, handled and tested for biological activity as described earlier (8 (link)). The human cervical cancer cell line SiHa harbouring HR-HPV16 DNA was cultured according to the supplier's protocol (American Type Culture Collection, Manassas, VA, USA). Growth medium (Dulbecco's modified Eagle's medium), supplements, phosphate-buffered saline (PBS) solution and the monoclonal antibody directed against the E7 protein of HPV16 were obtained from Invitrogen (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Monoclonal antibodies raised against the E6 protein of HPV16, human p53 (clone DO-1) and human p16^ink4a were acquired from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Anti-β-actin monoclonal antibodies, human plasma fibronectin (FN), bovine serum albumin (BSA, fraction V), doxorubicin hydrochloride and the chemicals employed for protein extraction were purchased from Sigma-Aldrich (St. Louis, MO, USA). The primers and probes employed for RNA analyses were purchased from Applied Biosystems (Thermo Fisher Scientific, Inc.).