Cd4 pe rpa t4

Manufactured by BD

CD4-PE (RPA-T4) is a fluorescently labeled antibody that binds to the CD4 surface marker on human T cells. It is intended for use in flow cytometry applications to identify and quantify CD4-positive cells within a sample.

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Lab products found in correlation

Facscanto 2, by BD (2 mentions) 4 1bb pe, by Thermo Fisher Scientific (1 mentions) Sh800s instrument, by Sony (1 mentions) Cd25 pe, by BioLegend (1 mentions) Anti human foxp3 staining set 236a e7, by Thermo Fisher Scientific (1 mentions) Cd59 pe, by BioLegend (1 mentions) Saponin, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-CD4-PE (clone RPA-T4), CD4-PE-CF594 (clone RPA-T4; PE-Cy5-anti-CD4 (clone RPA-T4) CD4 PE (clone RPA-T4, PE anti-human CD4 (clone RPA-T4; RPA-T4 CD4-PE

2 protocols using cd4 pe rpa t4

Isolation and Analysis of Mouse T Cell Subsets

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The anti-mouse TCRβ-fluorescein isothiocyanate (FITC) was purchased from BioLegend. CD4-PE (RPA-T4), and CD8-PE-Cy7 (SK1) were purchased from BD Biosciences. 4–1BB-PE were purchased from eBioscience. Flow cytometry data acquisition was performed on FACSCanto II (BD Biosciences) followed by the analysis of acquired data by FlowJo software (TreeStar). Co-cultures were sorted based on 4–1BB⁺ separately for CD3⁺CD4⁺CD8⁻ (CD4) and CD3⁺CD4⁻CD8⁺ (CD8) by SH800S instrument (Sony Biotechnology).

Paria B.C., Levin N., Lowery F., Pasetto A., Deniger D.C., Parkhurst M.R., Yossef R., Kim S.P., Florentin M., Ngo L., Ray S., Krishna S., Robbins P.F, & Rosenberg S.A. (2021). Rapid identification and evaluation of neoantigen-reactive T cell receptors from single cells.. Journal of immunotherapy (Hagerstown, Md. : 1997), 44(1), 1-8.

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Comprehensive Immunophenotyping of Endothelial Cells

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Flow cytometry was carried out on a FACS Canto II (BD Biosciences). Endothelial cells were phenotyped with the following antibodies: HLA-DR APC (L243), HLA-DQ PE (HLA-DQ1), HLA-ABC APC/Cy7 (W6/32), VE Cadherin PerCP/Cy5.5 (BV9), PD-L1 PE/Cy7 (29E.2A3), CD59 PE [P282 (H19)], CD55 FITC (JS11) and CD46 PE/Cy7 (TRA-2-10; Biolegend) and ICAM-1 FITC (84H10; Beckman Coulter). Lymphocyte polarization was analyzed using: CD4 PE (RPA-T4; BD Pharmingen), IFN-γ FITC (B27; BD Biosciences), CD3 PerCP (SK7; Becton Dickinson); CD4 PB (RPA-T4), CD8 PB (RPA-T8), CD45RA PE/Cy7 (H100), CD25 PE (M-A251), CD127 PerCP/Cy5.5 (A019D5), CD185 APC/Cy7 (J252D4; Biolegend) and IL-17 efluor660 (eBioscience). FoxP3 was detected using the anti-Human Foxp3 Staining Set (236A/E7; eBioscience). Intracellular staining of HLA-DR and HLA-DQ was carried out on fixed and permeabilised cells (2% PFA (Sigma) and 0.5% saponin (Sigma)). Intracellular staining of phosphorylated Akt was carried out in fixed and permeabilised cells (1.5% PFA followed by pure methanol (Millipore) at -20°C) using the following antibodies pAkt (Ser473) APC (SDRNR) and anti-mouse IgG Alexa Fluor^® 647 (Poly4053; Biolegend).

Cross A.R., Lion J., Poussin K., Glotz D, & Mooney N. (2021). Inflammation Determines the Capacity of Allogenic Endothelial Cells to Regulate Human Treg Expansion. Frontiers in Immunology, 12, 666531.

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