Mouse diabetes multiplex assay kit

Glucose oxidase/Peroxidase (PGO), WY14643, DNase I, collagenase, Percoll and Fao hepatoma cells were purchased from Sigma (St. Louis, MO, USA). Fao cells were derived from a rat hepatoma and no contamination was present. MK886 was obtained from Cayman Chemical (Ann Arbor, MI, USA). Rabbit anti-p-IGF-1Rβ and rabbit anti-IGF-1Rβ antibodies were obtained from Cell Signaling (Danvers, MA, USA). Rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and HRP-linked secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Goat anti-IGFBP-1 and the mouse anti-IGF-1 (total) Luminex kit were purchased from R&D Systems (Minneapolis, MN, USA). The mouse diabetes multiplex assay kit was obtained from Bio-Rad (Hercules, CA, USA). NEFA and triacylglycerol (TG) assay kits ware purchased from Wako Diagnostics (Richmond, VA, USA). FBS, NuPAGE gels, SuperScript III reverse transcriptase, Trizol, trypsin and the oligo(dT)_12–18 primer were obtained from Invitrogen (Carlsbad, CA, USA). Rabbit serum and diaminobenzidine were obtained from Vector Laboratories (Burlingame, CA, USA). All antibodies were validated by their manufacturers.

Glucose, insulin, T3, collagenase and Dulbecco’s modified Eagle’s medium (DMEM) were from Sigma-Aldrich (St. Louis, MO). Antibody against estrogen receptor α (ERα) and PE-conjugated anti-Sca-1 or PDGFRα antibodies were from R&D System (Minneapolis, MN). The anti-UCP1 antibody, FITC-conjugated antibody against CD29 or CD34, and mouse estradiol ELISA kit were purchased from the Abcam (Cambridge, MA). Anti-GAPDH antibody was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). NuPAGE gels, SuperScript III reverse transcriptase and oligo(dT)_12–18 primers were from Invitrogen (Carlsbad, CA). The mouse diabetes multiplex assay kit was from Bio-Rad (Hercules, CA). HF diet (60 kCal% from fat, 20 kCal% from protein, 20 kCal% from carbohydrate, energy density: 5.24 kCal/g, catalog number D12492) was from Research Diets, Inc (New Brunswick, NJ). Regular chow (17 kCal% from fat, 25 kCal% from protein, 58 kCal% from carbohydrate, energy density: 3.1 kCal/g, catalog number 7912) was from Harlan Laboratories (Madison, WI).