All serological analyses were performed in Germany after transfer of the samples from Tanzania. Serum samples were available for analysis in 598 of 600 participants. All serum samples were tested for HBs-antigen, anti-HBc antibodies and quantitative anti-HBs-antibodies. In addition, HBs-antigen-positive samples were tested for anti-HBc IgM antibodies, HBe-antigen and anti-HBe antibodies. The following test kits were used for HBV serology: Enzygnost HBsAg 6.0 (Siemens Healthcare, Marburg, Germany), Enzygnost anti-HBc monoclonal (Siemens), Architect Anti-HBs (Abbott, Wiesbaden, Germany), and Architect Anti-HBc-IgM. All 598 serum samples were screened for hepatitis C antibodies. Anti-HCV-positive samples were analyzed by PCR for HCV RNA. HCV antibodies were identified using the Architect Anti-HCV assay (Abbott). In addition, 592 serum samples were tested with further anti-HBs antibody test (Hepatitis B antibody test no. BSB3120001, SureScreen Diagnostics, Derby, Great Britain), which was an easy-to-use point-of-care test in cassette format. The test utilizes a double antigen sandwich system and detects anti-HBs levels as low as 10 U/mL in whole blood, serum or plasma.
Enzygnost anti hbc monoclonal
Manufactured by Siemens
Sourced in Germany
Enzygnost anti-HBc monoclonal is a laboratory test kit used to detect the presence of antibodies against the hepatitis B core antigen (anti-HBc) in human serum or plasma samples. It is designed to aid in the diagnosis of hepatitis B virus infection.
Automatically generated - may contain errors
2 protocols using enzygnost anti hbc monoclonal
1
Comprehensive Hepatitis B Serological Screening
2
Serological Screening Protocols for Donated Blood
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Laboratory testing was performed at the Serology Division of the Fundação Pró-São Paulo Hemocentro de São Paulo following usual blood bank procedures for HBV surface antigen, HBV core antibody, syphilis, anti-HCV, anti-HTLV-1/2, Chagas disease, and anti-HIV-1 and -2. Serologic reactive units were discarded according to institution policy.14 (link) Donors who presented any positive screening test results were contacted to collect another sample.
The Enzygnost HBsAg 5.0 (Siemens, Marburg, Germany) and Enzygnost Anti-HBc monoclonal (Siemens, Marburg, Germany) tests were used for HBV screening and confirmatory tests and the Murex anti-HCV version 4.0 (Abbott, South Africa) test was used for HCV screening. An immunoblot (CHIRON RIBA HCV 3.0 S/A, Emeryville, USA) was used for the HCV confirmatory test.
The Enzygnost HBsAg 5.0 (Siemens, Marburg, Germany) and Enzygnost Anti-HBc monoclonal (Siemens, Marburg, Germany) tests were used for HBV screening and confirmatory tests and the Murex anti-HCV version 4.0 (Abbott, South Africa) test was used for HCV screening. An immunoblot (CHIRON RIBA HCV 3.0 S/A, Emeryville, USA) was used for the HCV confirmatory test.
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