The fixed proximal portion of the penis of 6 of 13 sham_SHR and 8 of 12 ESWT_SHR was paraffin embedded and 5-μm sections were performed using a microtome (RM 2245; Leica Biosystems, Nanterre, France). The sections were positioned on SuperFrost plus glass slides (Thermo Fisher Scientific, Waltham, MA, USA), dried, and stained for evaluation of smooth muscle/collagen ratio with Masson’s trichrome method using an automated multistainer (ST5020 &CV5030 Coverslipper; Leica Biosystems). The acquisition of slides images was then performed using Aperio AT2 - Scanner for On-Screen Diagnosis (Leica Biosystems). The percent of smooth muscle and the percent of collagen were computed in the total area of CC from each section by a blinded experimenter using the positive pixel count algorithm (Leica Biosystems). For each animal, 4 transverse sections of penis were stained and analyzed and a mean smooth muscle/collagen ratio was determined.
St5020 cv5030 coverslipper
Manufactured by Leica
Sourced in United States, France
The ST5020 and CV5030 are automated coverslipper instruments designed for laboratory use. The ST5020 is a slide coverslipper that automatically applies coverslips to prepared slides. The CV5030 is a coverslipper that automatically applies coverslips to microscope slides. Both products are intended to streamline the slide preparation process in laboratory settings.
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Lab products found in correlation
2 protocols using st5020 cv5030 coverslipper
1
Penile Smooth Muscle and Collagen Analysis
2
Quantitative Histomorphometric Analysis of Rat Bladder
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The rats were euthanized and the bladder was harvested, paraffin embedded and 5-μm sections were performed using a microtome (RM 2245®, Leica Biosystems, France). The sections were stained with hematoxylin-eosin using an automated multistainer (ST5020® &CV5030 Coverslipper® Leica Biosystems, France) for evaluation of detrusor wall area, the detrusor being sandwiched between the mucosa and adventitia. The acquisition of slides images was then performed using Aperio AT2 - Scanner for On-Screen Diagnosis® (Leica Biosystems, France). Then images were analyzed with NIS Elements Software (NIS elements, Nikon, France) to compute the detrusor wall area from each section by a blinded experimenter. For each animal, 4 transverse sections were analyzed and a mean area of sections per animal was then determined.
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