Proxeon trap column

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Proxeon trap column is a chromatographic column used for sample cleanup and concentration in liquid chromatography-mass spectrometry (LC-MS) applications. It serves as a sample pre-treatment device to remove unwanted contaminants and enrich target analytes prior to separation and detection.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using proxeon trap column

Reversed-Phase Chromatography for Peptide Separation

Check if the same lab product or an alternative is used in the 5 most similar protocols

An online reversed-phase chromatography was used to separate the sample, through a Thermo Scientific Proxeon easy nLC1000 equipped with a Proxeon trap column (100 μm ID x 2 cm, Thermo Scientific) and a C18 packed-tip column (Acclaim PepMap, 75 µm ID x 15 cm, Thermo Scientific). Peptides were separated with an increasing amount of ACN (2%-40% over 60 min) at a flow rate of 300 nL/min. The peptides were electrosprayed directly from the analytical column and a voltage of 1.7 kV was applied via the liquid junction of the nanospray source. The chromatography system was coupled to the mass spectrometer Thermo Scientific Q-exactive programmed with a top 10 data-dependent mode for all the samples. The resolving power was 70,000 FWHM (m/z 400), in a positive mode and using an AGC target of 3^e6. Default charge state was set at 2, unassigned and +1 charge states were rejected and dynamic exclusion was enabled for 25 s. The scan range was set to 300-1600 m/z. For ddMS², the scan range was between 200 and 2000 m/z, 1 microscan was acquired at 17,500 FWHM and an isolation window of 4.0 m/z was used.

Capuz A., Karnoub M.A., Osien S., Rose M., Mériaux C., Fournier I., Devos D., Vanden Abeele F., Rodet F., Cizkova D, & Salzet M. (2022). The Antibody Dependant Neurite Outgrowth Modulation Response Involvement in Spinal Cord Injury. Frontiers in Immunology, 13, 882830.

+ Open protocol

+ Expand

Peptide Separation and Mass Spectrometry Analysis

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were separated by online reversed-phase chromatography using a Thermo Scientific Proxeon Easy-nLC system equipped with a Proxeon trap column (100 μm ID × 2 cm, Thermo Scientific) and a C18 packed-tip column (100 μm ID × 10 cm, Nikkyo Technos Co. Ltd). Peptides were separated using an increasing amount of acetonitrile (5%–30% over 120 minutes) at a flow rate of 300 nL/min. The LC eluent was electrosprayed directly from the analytical column and a voltage of 1.6 kV was applied via the liquid junction of the nanospray source. The chromatography system was coupled to a Thermo Scientific LTQ-Orbitrap XL mass spectrometer. The LTQ-Orbitrap XL instrument was set to acquire top 20 MS/MS in data-dependent mode. The survey scans were taken at 70,000 full width at half maximum (FWHM) (at m/z 400) resolving power in positive mode and using a target of 3E6 and default charge state of 2. Unassigned and +1 charge states were rejected, and dynamic exclusion was enabled for 20 s. The scan range was set to 300–1600 m/z. For the MS/MS, 1 microscan was obtained at 17,500 FWHM and isolation window of 4.0 m/z, using a scan range between 200–2000 m/z 30 (link)32 (link).

Grulova I., Slovinska L., Blaško J., Devaux S., Wisztorski M., Salzet M., Fournier I., Kryukov O., Cohen S, & Cizkova D. (2015). Delivery of Alginate Scaffold Releasing Two Trophic Factors for Spinal Cord Injury Repair. Scientific Reports, 5, 13702.

+ Open protocol

+ Expand

Liquid Chromatography-Mass Spectrometry Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were separated by online reversed-phase chromatography using a Thermo Scientific Proxeon Easy-nLC system equipped with a Proxeon trap column (100 μm ID × 2 cm, Thermo Scientific) and a C18 packed-tip column (100 μm ID × 15 cm, Nikkyo Technos Co. Ltd.). Peptides were separated using an increasing amount of acetonitrile (5–40% over 110 min) at a flow rate of 300 nL/min. The LC eluent was electrosprayed directly from the analytical column and a voltage of 1.7 kV was applied via the liquid junction of the nanospray source. The chromatography system was coupled to a Thermo Scientific Orbitrap Elite mass spectrometer programmed to acquire in a data-dependent mode. The survey scans were acquired in the Orbitrap mass analyzer operated at 120,000 (FWHM) resolving power. A mass range of 400–2000 m/z and a target of 1E6 ions were used for the survey scans. Precursors observed with an intensity over 500 counts were selected “on the fly” for ion trap collision-induced dissociation (CID) fragmentation with an isolation window of 2 amu and a normalized collision energy of 35%. A target of 5000 ions and a maximum injection time of 200 ms were used for CID MS² spectra. The method was set to analyze the 20 most intense ions from the survey scan and dynamic exclusion was enabled for 20 s.

Cizkova D., Le Marrec-Croq F., Franck J., Slovinska L., Grulova I., Devaux S., Lefebvre C., Fournier I, & Salzet M. (2014). Alterations of protein composition along the rostro-caudal axis after spinal cord injury: proteomic, in vitro and in vivo analyses. Frontiers in Cellular Neuroscience, 8, 105.

+ Open protocol

+ Expand

Reversed-Phase Chromatography and Q-Exactive Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were separated by online reversed-phase chromatography using a Thermo Scientific Proxeon Easy-nLC1000 system equipped with a Proxeon trap column (100 μm ID × 2 cm, Thermo Scientific) and a C18 packed-tip column (Acclaim PepMap, 75 µm ID × 15 cm, Thermo Scientific). Peptides were separated using an increasing amount of acetonitrile (5–35% over 120 min) at a flow rate of 300 nL/min. The LC eluate was electrosprayed directly from the analytical column and a voltage of 1.7 kV was applied via the liquid junction of the nanospray source. The chromatography system was coupled to a Thermo Scientific Q-Exactive mass spectrometer programmed to acquire in a data-dependent mode the Top 10 most intense ion method. The survey scans were done at a resolving power of 70,000 FWHM (m/z 400), in positive mode and using an AGC target of 3^e6. Default charge state was set at 2, unassigned and +1 charge states were rejected, and dynamic exclusion was enabled for 25 s. The scan range was set to 300–1600 m/z. For ddMS², the scan range was between 200–2000 m/z, 1 Microscan was acquired at 17,500 FWHM and an isolation window of 4.0 m/z was used.

Capuz A., Osien S., Cardon T., Karnoub M.A., Aboulouard S., Raffo-Romero A., Duhamel M., Cizkova D., Trerotola M., Devos D., Kobeissy F., Van den Abelle F., Bonnefond A., Fournier I., Rodet F, & Salzet M. (2023). Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes: a new player in neural proteome. Cell Death & Disease, 14(8), 526.

+ Open protocol

+ Expand

Peptide Separation and Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The samples were separated by online reversed-phase chromatography using a Thermo Scientific Easy-nLC1000 system equipped with a Proxeon trap column (75 μm ID × 2 cm, 3 μm, Thermo Scientific,) and a C18 packed-tip column (Acclaim PepMap, 75 μm ID × 50 cm, 2 μm, Thermo Scientific). The digested peptides were separated using an increasing amount of acetonitrile in 0.1% formic acid from 2 to 30% for 2 h at a flow rate of 300 nL/min. A voltage of 2.4 kV was applied by the liquid junction to electrospray the eluent using the nanospray source. A high-resolution mass spectrometer Q-Exactive^TM Thermo Scientific^TM was coupled to the chromatography system to acquire the ten most intense ions of MS1 analysis (Top 10) in a data-dependent mode. The MS analyses were performed in positive mode at a resolving power of 70,000 FWHM, using an automatic gain control target of 3e6, the default charge state was set at 2 and a maximum injection time at 120 ms. For full-scan MS, the scan range was set between m/z 300 and 1600. For ddMS², the scan range was between m/z 200 to 2000, 1 Microscan was acquired at 17,500 FWHM, an AGC was set at 5e4 ions and an isolation window of m/z 4.0 was used.

Capuz A., Osien S., Karnoub M.A., Aboulouard S., Laurent E., Coyaud E., Raffo-Romero A., Duhamel M., Bonnefond A., Derhourhi M., Trerotola M., El Yazidi-Belkoura I., Devos D., Zilkova M., Kobeissy F., Vanden Abeele F., Fournier I., Cizkova D., Rodet F, & Salzet M. (2023). Astrocytes express aberrant immunoglobulins as putative gatekeeper of astrocytes to neuronal progenitor conversion. Cell Death & Disease, 14(4), 237.

+ Open protocol

+ Expand

Nanoflow LC-MS/MS Peptide Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The digested peptides were reconstituted with an acetonitrile/formic acid solution (2%/0.1%) (Biosolve B. V. Valkensvaard, Netherlands/Fluka^TM). Samples were separated at a flow rate of 300 nL/min, by an online reversed-phase chromatographic system (Easy-nLC 1000 UPLC system, Thermo Scientific, Waltham, MA, USA) equipped with a Proxeon trap column (100 µm internal diameter × 2 cm, Thermo Scientific) and a C₁₈ packed capillary column (Acclaim PepMap 100 C18, 75 µm ID × 50 cm, Thermo Scientific). The LC eluent was electrosprayed directly from the analytical column, and a voltage of 1.7 kV was applied via the liquid junction of the nanospray source. The chromatographic system was interfaced to a Q Exactive mass spectrometer (Thermo Scientific) set to acquire a top 10 MS² in a data-dependent mode. The survey scans were conducted at a resolving power of 70 000 FWHM (m/z 400) in the positive mode and using an automatic gain control target of 5 × 10⁶. Default charge state was set at 2, unassigned and +1 charge states were rejected, and dynamic exclusion was enabled for 20 s. The scan range was set to 300–1600 m/z. For ddMS², the scan range was 200–2000 m/z; one microscan was acquired at 17,500 FWHM, and an isolation window of 4.0 m/z was used.

Nekoua M.P., Bertin A., Sane F., Gimeno J.P., Fournier I., Salzet M., Engelmann I., Alidjinou E.K, & Hober D. (2021). Persistence of Coxsackievirus B4 in Pancreatic β Cells Disturbs Insulin Maturation, Pattern of Cellular Proteins, and DNA Methylation. Microorganisms, 9(6), 1125.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!