Ixon emccd camera

Manufactured by Oxford Instruments

Sourced in United Kingdom, Japan, Ireland

The IXon EMCCD camera is a high-performance scientific imaging device designed for low-light applications. It features an electron-multiplying CCD sensor that amplifies the signal, enabling it to detect even single photon events with high sensitivity. The camera is capable of capturing images with low noise and fast frame rates, making it suitable for a variety of scientific research and industrial applications.

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Lab products found in correlation

Ix71 microscope, by Olympus (14 mentions) Metamorph, by Molecular Devices (9 mentions) Ix81 inverted microscope, by Olympus (6 mentions) Celltirf 4line system, by Olympus (6 mentions) Fm4 64, by Thermo Fisher Scientific (5 mentions) Csu x1 scanner, by Yokogawa (5 mentions) Eclipse ti microscope, by Nikon (4 mentions) Metamorph advanced imaging acquisition software v 7, by Molecular Devices (4 mentions) Spinning disc scan head, by Yokogawa (4 mentions) Spinning disc scan head, by Olympus (4 mentions) Plan apochromat 1, by Oxford Instruments (4 mentions) Bx51w1 microscope, by Olympus (4 mentions) On stage culture chamber, by Tokai Hit (4 mentions) Zen software, by Zeiss (3 mentions) Leibovitz s l 15 medium, by Thermo Fisher Scientific (3 mentions) Elyra system s 1 microscope, by Zeiss (3 mentions) Eclipse ti e microscope, by Nikon (3 mentions) Csu x1 spinning disk confocal head, by Yokogawa (3 mentions) Metamorph software, by Molecular Devices (3 mentions) Lumplfl100xw, by Olympus (3 mentions)

Close spelling variants of this product

EMCCD camera (203 citations) IXon 897 EMCCD camera (61 citations) IXon Ultra EMCCD camera (60 citations) IXon Ultra 888 EMCCD camera (34 citations) IXon Ultra 897 EMCCD camera (33 citations) IXon DU-897 EMCCD camera (22 citations) Andor iXon EMCCD camera (14 citations) IXon X3 EMCCD camera (10 citations) IXon Life 888 EMCCD camera (10 citations) Andor iXon Ultra 897BV EMCCD camera (6 citations)

125 protocols using ixon emccd camera

Live Embryo Imaging with Confocal SIM

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Images of fixed samples were taken on Zeiss LSM 700 confocal microscope with X63/1.4 PlanApo objective. Superresolution images were obtained using Elyra S.1 structured illumination system (SIM) with X63/1.4 PlanApo objective and EMCCD iXon camera (Andor). Confocal or SIM z stacks were analyzed and processed by Zen software (Zeiss).
For the imaging of live embryos, gravid adult hermaphrodites were dissected in 50 μl of 0.7× egg salts and embryos were moved to 2% agar pad with a mouth pipette. The specimen was covered with a coverslip (1.5H) and sealed with scotch tape to prevent drying. Images were acquired using spinning disc Revolution XD confocal system (Andor) based on Nikon Eclipse Ti microscope equipped with 100×/1.3 PlanFluor objective, perfect focus system; CSU-X1 spinning disc (Yokogawa) and iXon3 EMCCD camera (Andor) operated by IQ2 software (Andor). Image sequences were further processed and analyzed with ImageJ (NIH). Figures were prepared using Adobe Photoshop CS5 and Adobe Illustrator CS11.

Smurova K, & Podbilewicz B. (2016). RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion. Cell Reports, 14(6), 1517-1527.

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Super-Resolution Imaging of NMDAR

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Live hippocampal neurons were incubated with Healthy + or PSY + NMDAR-Ab for 2 h at 37 °C. Surface endogenous GluN2A-containing NMDAR were specifically stained using an anti-GluN2A antibody (0.1 mg ml⁻¹, 15 min). Cells were then successively incubated with an anti-PSD95 antibody (0.1 mg ml⁻¹, 45 min), secondary anti-rabbit Alexa 647 (Invitrogen, 0.1 mg ml⁻¹, 30 min), and anti-mouse Alexa 532 (0.1 mg ml⁻¹, 30 min) antibodies. A second fixation was performed after incubation with the secondary antibodies. All imaging sessions were performed using a Leica SR GSD 3D microscope (Leica HC PL APO 160 × 1.43 numerical aperture oil-immersion total internal reflection fluorescence objective) and an ANDOR EMCCD iXon camera. Localization of single molecules and reconstruction of the super-resolved image was performed by applying a fitting algorithm determining the centroid-coordinates of a single molecule and fitting the point-spread-function of a distinct diffraction limited event to a Gaussian function. The final achieved spatial resolution was 40 nm.

Jézéquel J., Johansson E.M., Dupuis J.P., Rogemond V., Gréa H., Kellermayer B., Hamdani N., Le Guen E., Rabu C., Lepleux M., Spatola M., Mathias E., Bouchet D., Ramsey A.J., Yolken R.H., Tamouza R., Dalmau J., Honnorat J., Leboyer M, & Groc L. (2017). Dynamic disorganization of synaptic NMDA receptors triggered by autoantibodies from psychotic patients. Nature Communications, 8, 1791.

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Super-resolution Imaging of Fixed Cells

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According to an established method (Nahidiazar et al., 2015 (link)), cells for superresolution microscopy were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100, blocked with PBS containing 5% BSA (Sigma-Aldrich), and subsequently incubated with primary and secondary antibodies with washing steps in between. Imaging was performed with a Leica SR-GSD microscope (Leica Microsystems) equipped with 405-nm/30-mW, 488-nm/300-mW, and 642-nm/500-mW lasers, with samples immersed in OxEA buffer (Nahidiazar et al., 2016 (link)). A 160× oil-immersion objective (NA 1.47) and an EMCCD iXon camera (Andor Technology) were used to collect images. Between 10,000 and 50,000 frames were collected, with a frame rate of 100 Hz. All the datasets were analyzed with the ThunderSTORM module of ImageJ software (Ovesný et al., 2014 (link)).

Wang W., Zuidema A., te Molder L., Nahidiazar L., Hoekman L., Schmidt T., Coppola S, & Sonnenberg A. (2020). Hemidesmosomes modulate force generation via focal adhesions. The Journal of Cell Biology, 219(2), e201904137.

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Localization of Membrane-Bound Protein via Fluorescence Microscopy

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To verify the localization of DgcP_msfGFP fusions, fluorescence microscopy was performed using a Nikon Eclipse TiE microscope equipped with a 25-mm SmartShutter and an Andor EMCCD i-Xon camera. For fluorescence microscopy and bright field microscopy, a Plan APO VC Nikon 100X objective (NA = 1.4) and a Plan Fluor Nikon 40X objective (NA = 1.3) were used. For membrane staining, cells were treated with 50 µg/mL FM4-64 (Invitrogen). For phase contrast microscopy, a Plan APO λ OFN25 Nikon 100X objective (NA = 1.45) was used. All microscopy assays were performed with immobilized cells on 25% LB pads with 1.5% agarose. Image analyses were performed using the ImageJ^{60 (link)} and MicrobeJ^{61 (link)} softwares.

Nicastro G.G., Kaihami G.H., Pulschen A.A., Hernandez-Montelongo J., Boechat A.L., de Oliveira Pereira T., Rosa C.G., Stefanello E., Colepicolo P., Bordi C, & Baldini R.L. (2020). c-di-GMP-related phenotypes are modulated by the interaction between a diguanylate cyclase and a polar hub protein. Scientific Reports, 10, 3077.

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Live-Cell Imaging of Centrosome and Kinetochore

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Cells were cultured on 22-mm square coverslips and mounted in a custom Rose chamber or grown on 35-mm glass-bottomed plates. Cells were imaged in Leibovitz’s L-15 medium (Gibco) supplemented with 10% FBS and 2 mM l-glutamine at 35–37°C.
Confocal GFP fluorescence micrographs of hTERT-RPE1 cells expressing GFP-Centrin/GFP-CENP-A were acquired using a Nikon TE2000 microscope (Morrell Instruments) with a 100× oil objective (PlanApo, 1.4 NA) equipped with a z piezo stage. With 0.4-µm spacing between z planes, micrographs were taken through the entire cell with a PerkinElmer Wallac UltraView confocal head and 488-nm excitation laser (Coherent). Images were acquired with an sCMOS Prime95B camera (Photometrics) using NIS-Elements software (Nikon).
Confocal fluorescence and differential interference contrast micrographs were acquired using an Inverted Axiovert 200 microscope (Zeiss/Perkin-Elmer) with a 100× oil objective (PlanApo, 1.4 NA). Images consisting of single z planes were taken with a PerkinElmer Wallac UltraView confocal head using solid-state 491-nm and/or 644-nm lasers for excitation (Spectral Applied). Images were acquired with an EMCCD iXon camera (Andor) using Metamorph software (MDS Analytical Technologies).

Pamula M.C., Carlini L., Forth S., Verma P., Suresh S., Legant W.R., Khodjakov A., Betzig E, & Kapoor T.M. (2019). High-resolution imaging reveals how the spindle midzone impacts chromosome movement. The Journal of Cell Biology, 218(8), 2529-2544.

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FRAP Imaging of H1.0/H1e-GFP Dynamics

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Imaging was done as described previously (Melcer et al., 2012 (link)). In brief, we used a Revolution spinning disk (CSUX; Yokogawa) imaging system (Andor) mounted on an IX81 fully automated microscope (Olympus) equipped with an automated stage and an environmental chamber (LIS) controlling humidity, CO₂, and temperature. FRAP was done using a specialized FRAPPA module (Andor) at 100% 488-nm solid-state laser (50-mW) intensity. We used an EMCCD iXon+ camera (Andor) with a window size of 512 × 512 pixels. H1.0/H1e-GFP recovery was measured over 45–90 s at one or two frames per second. All FRAP experiments were completed within 1 h after addition of KCl into the culture media. The FRAP analyses were performed on 15–30 cells from at least two independent experiments. Two-tailed Student’s t test was performed to compare the kinetics of the different FRAP curves.

Azad G.K., Ito K., Sailaja B.S., Biran A., Nissim-Rafinia M., Yamada Y., Brown D.T., Takizawa T, & Meshorer E. (2018). PARP1-dependent eviction of the linker histone H1 mediates immediate early gene expression during neuronal activation. The Journal of Cell Biology, 217(2), 473-481.

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Immunolocalization of IZUMO1 Protein

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The localization of IZUMO1 was determined by immunostaining. Briefly, after fixation cells were permeabilized with 0.1% Triton X-100 in PBS and incubated with anti-IZUMO1, clone Mab120 (1:500, Cat# MABT1357; Merck Millipore) followed by the secondary antibody Alexa Fluor 647 goat anti-rat (1:500, Cat# A-21247; Thermo Fisher Scientific, RRID: AB_141778). Later, the nuclei were stained with 1 µg/ml DAPI and micrographs were obtained using wide-field illumination using an ELYRA system S.1 microscope (Plan-Apochromat 20x NA 0.8; Zeiss) with an EMCCD iXon camera (Andor) through ZEN microscopy software 7.0.4.0 (RRID: SCR_013672; Zeiss).

Brukman N.G., Valansi C, & Podbilewicz B. (2024). Sperm induction of somatic cell-cell fusion as a novel functional test. eLife, 13, e94228.

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Live T-cell Imaging via TIRF Microscopy

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We conducted bright-field microscopy of live T cells, using an eclipse Ti Nikon microscope, equipped with a (CFI-SR-HP) Apochromat TIRF X100 NA 1.49 (WD 0.12 mm) oil immersion objective (Nikon Instruments, Melville, NY, USA). Images were collected, using an EMCCD Ixon + camera (Andor, Belfast, UK). The pixel width was 160 nm. Image stacks were generated by taking 200 serial images with acquisition time of 200 ms per frame. Image binning of 8 bit was used for GLIE analysis.

Deutsch M., Yakovian O., & Sherman E. (2020). Fluctuations in the Homogeneity of Cell Medium Distinguish Benign from Malignant Lymphocytes in a Cellular Model of Acute T Cells Leukemia. Applied Sciences, 10(24), 8894-8894.

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Microscopic Analysis of Membrane Integrity

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Microscopy was performed using a Nikon Eclipse TiE microscope equipped with a Plan APO VC Nikon 100X objective (NA = 1.4), a 25-mm SmartShutter, and Andor EMCCD i-Xon camera. For fluorescence observations, strains were grown and starved for fatty acids as described above, concentrated ten fold in fresh LB and treated with 50 µg.mL -1 of FM1-43 (Invitrogen TM ) and 10 µg. mL -1 of propidium iodide to evaluate membrane damage and permeability. At least 200 cells were examined for each experimental condition. For cell size estimation at least 200 cells of each strain were measured using ImageJ (http://rsb.info.nih.gov/ij/). For observation of the cell wall, cells were resuspended with 50 µg.mL -1 of FM4-64 (Invitrogen TM ) and 10 µg.mL -1 of WGA-Alexa Fluor 350 (Invitrogen TM ). For MinD localization, cells were grown in LB with 0.5% xylose for MinD-YFP expression until OD 600 ~ 0.4 and then used as inoculum in fatty acid starvation experiments, as described above. All microscopy was performed with cells immobilized on LB 25% (LB diluted four times) agarose pads, solidified with 1.5% (w/vol) agarose.

Pulschen A.A., Sastre D.E., Machinandiarena F., Crotta Asis A., Albanesi D., de Mendoza D, & Gueiros-Filho F.J. (2017). The stringent response plays a key role in Bacillus subtilis survival of fatty acid starvation. Molecular microbiology, 103(4).

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Imaging NEMO Condensate Dynamics

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Cells were grown on chambered cover glasses to 70–80% confluency, at which time cells were stimulated with IL-1β (1 μg/ml) or TNFα (1 μg/ml). Live cell images were immediately captured every 10 seconds for 1 hour (IL-1β stimulation) or 30 min (TNFα stimulation) under an Andor spinning disk confocal microscope system equipped with a Nikon Ti motorized microscope (with 60× oil objective), a CSUX1 Spinning Disk Confocal head (Yokogawa), an Andor iXon EMCCD camera and a Neo sCMOS camera. Images were analyzed by ImageJ. To quantify the number of cellular condensates, backgrounds of images were first subtracted by using the module of subtract background on ImageJ, and then condensates (~ 0.3–1 μm in diameter) in cells were spotted and counted using the plugin of SpotCounter on ImageJ (the threshold of fluorescent intensity in detecting NEMO puncta was set above that of free NEMO). For tracking the fusion of two condensates as shown in Figure 2E–F, trajectories and positions of condensates were obtained using the plugin of TrackMate on ImageJ. Cells that contained NEMO puncta after IL-1β or TNFα treatment represented > 90% of stimulated cells.

Du M., Ea C.K., Fang Y, & Chen Z.J. (2022). Liquid Phase Separation of NEMO Induced by Polyubiquitin Chains Activates NF-κB. Molecular cell, 82(13), 2415-2426.e5.

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