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GraphPad Software package is a comprehensive suite of data analysis and visualization tools designed for scientific and research applications. The software provides a range of functions, including curve fitting, statistical analysis, and graphing capabilities, to aid researchers in interpreting their data and presenting their findings.

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PRISM software package (29 citations) Prism statistical software package (6 citations) Prism® 5 Software Package (6 citations) InStat software package (4 citations) GraphPad Prism Software Package, version 6.0 (3 citations) QuickCals software package (2 citations) Graph software package Prism5 (2 citations) GraphPad statistical software package, version 6.0 (2 citations) Prizm software package, version 4 (2 citations) GraphPad Prism 7 Software Statistical Package (2 citations)

15 protocols using software package

1

Enzymatic Activity Assay Protocol

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All statistical analyses and curves were performed using the GraphPad software package (La Jolla, CA, USA). Statistical significance between conditions was calculated using a t-test (two-tailed) when comparing two groups. All the enzymatic activity assays were performed in triplicate and quantified data were presented as mean ± SD. IC50 values were calculated using non-linear regression and shown as data points representing mean ± SD.
Qin Y., Li D., Qi C., Xiang H., Meng H., Liu J., Zhou S., Gong X., Li Y., Xu G., Zu R., Xie H., Xu Y., Xu G., Zhang Z., Chen S., Pan L., Li Y, & Tan L. (2023). Structure-based development of potent and selective type-II kinase inhibitors of RIPK1. Acta Pharmaceutica Sinica. B, 14(1), 319-334.
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2

Statistical Analysis of Experimental Data

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Statistical analyses were conducted using the GraphPad Software package (v7.0, GraphPad Software, USA). Results were analyzed by one-way analyses of variance (ANOVA) followed by Dunnett’s multiple-comparison test or Tukey’s multiple-comparison test. The mean values ± standard deviations (SD) are shown in the figures and statistical significance was established at p < 0.05.
Schüroff P.A., Salvador F.A., Abe C.M., Wami H.T., Carvalho E., Hernandes R.T., Dobrindt U., Gomes T.A, & Elias W.P. (2021). The aggregate-forming pili (AFP) mediates the aggregative adherence of a hybrid-pathogenic Escherichia coli (UPEC/EAEC) isolated from a urinary tract infection. Virulence, 12(1), 3073-3093.
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3

Predicting G-Quadruplex Motifs in CRISPR gRNAs

Cited 2 times
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All 1,841 gRNAs sequences targeting the CDS of selected genes and their corresponding percent ranks were taken as published in Supplementary Table 7 from Doench et al.8 (link) For prediction of G-Q tetrad motifs sequences were scored as containing putative G-Q structures if they followed the tetrad pattern of GxNy1GxNy2GxNy3Gx, where x≥2 and y1, y2, y3 can be any nucleotide of any gap length, with only one gap length among the three being zero in length being permissible18 (link). Kolmogorov–Smirnov t-test and boxplots were calculated and visualized using Graphpad software package (GraphPad Software, Inc.).
Malina A., Cameron C.J., Robert F., Blanchette M., Dostie J, & Pelletier J. (2015). PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing. Nature Communications, 6, 10124.
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4

Comprehensive Statistical Analyses of Bacterial Behavior

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Statistical analyses were conducted using the GraphPad Software package (GraphPad Software, La Jolla, CA, USA). One-way analysis of variance (ANOVA) and two-way ANOVA were used to analyze biofilm formation, bacterial adherence, invasion data and bactericidal serum, and qRT-PCR results, respectively. The comparison of the bacterial loads in the animal infection study were evaluated by the nonparametric Mann–Whitney U Test. The mean values are shown in the figures. Statistical significance was considered at P < 0.05.
Zhang Y., Wang Y., Zhu H., Yi Z., Afayibo D.J., Tao C., Li T., Tian M., Qi J., Ding C., Yu S, & Wang S. (2021). DctR contributes to the virulence of avian pathogenic Escherichia coli through regulation of type III secretion system 2 expression. Veterinary Research, 52, 101.
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5

Statistical Analysis of Experimental Data

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The Graph-Pad software package (version 4.03, GraphPad Software Inc., San Diego, California, USA) was used to perform the non-parametric Mann-Whitney test or the parametric Student’s t test to represent data with bar graphs and to illustrate mean values and standard deviations. Significant differences were highlighted with asterisks (*p < 0.05; **p < 0.01; ***p < 0.005).
Fittschen M., Lastres-Becker I., Halbach M.V., Damrath E., Gispert S., Azizov M., Walter M., Müller S, & Auburger G. (2015). Genetic ablation of ataxin-2 increases several global translation factors in their transcript abundance but decreases translation rate. Neurogenetics, 16(3), 181-192.
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6

Statistical Analysis of UPEC Strains

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The GraphPad Software package (v7.0, GraphPad Software, USA) was used for statistical analyses. The Student’s t-test was employed to statistically compare UPEC-46 (wild-type) and derivative strains. Multiple comparisons were performed using the one-way analysis of variance (ANOVA) with post hoc Tukey HSD (honestly significant difference) test. The figures show the mean values ± standard deviations (SD) and p values <0.05 were considered statistically significant.
Schüroff P.A., Abe C.M., Silva J.W., de Paula Coelho C., Andrade F.B., Hernandes R.T., Dobrindt U., Gomes T.A, & Elias W.P. (2022). Role of aggregate-forming pilus (AFP) in adherence and colonization of both intestinal and urinary tracts. Virulence, 13(1), 1423-1433.
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7

Statistical Analyses for Biological Assays

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Statistical analyses were conducted using the GraphPad Software package (GraphPad Software, La Jolla, CA, USA). One-way analysis of variance (ANOVA) was used to analyze motility and invasion assay data, and two-way ANOVA was performed to analyze the survival assays and qRT-PCR results. The animal infection data were analyzed using the non-parametric Mann–Whitney U-test. The mean values are shown in the figures. Statistical significance was established at p < 0.05.
Wang S., Liu X., Xu X., Yang D., Wang D., Han X., Shi Y., Tian M., Ding C., Peng D, & Yu S. (2016). Escherichia coli Type III Secretion System 2 ATPase EivC Is Involved in the Motility and Virulence of Avian Pathogenic Escherichia coli. Frontiers in Microbiology, 7, 1387.
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8

Statistical Analysis of In Vitro and In Vivo Experiments

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Statistical analysis for in vitro and in vivo experiments used GraphPad Software package (GraphPad Software, La Jolla, CA, USA). One-way ANOVA was used for analysis of invasion assay in vitro data. Two-way ANOVA was performed on qRT-PCR results. Animal infection study analysis was performed using the nonparametric Mann-Whitney U-Test. Statistical significance was established at p < 0.05.
Wang S., Bao Y., Meng Q., Xia Y., Zhao Y., Wang Y., Tang F., ZhuGe X., Yu S., Han X., Dai J, & Lu C. (2015). IbeR Facilitates Stress-Resistance, Invasion and Pathogenicity of Avian Pathogenic Escherichia coli. PLoS ONE, 10(3), e0119698.
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9

Comparative Analysis of Experimental Groups

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One-way ANOVA for multiple group comparisons was used to assess the significance of differences. Results were presented as mean ± s.e.m. In all cases, P < 0.05 was considered statistically significant. All analytic results were performed using the GraphPad Software package (GraphPad Software, La Jolla, CA, USA).
Zang Z.J., Tang H.F., Tuo Y., Xing W.J., Ji S.Y., Gao Y, & Deng C.H. (2015). Effects of velvet antler polypeptide on sexual behavior and testosterone synthesis in aging male mice. Asian Journal of Andrology, 18(4), 613-619.
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10

Statistical Analyses of Biological Assays

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Statistical analyses used the GraphPad Software package (GraphPad Software, La Jolla, CA, USA). One-way analysis of variance (ANOVA) was used for analysis of adhesion assay data, and two-way ANOVA was performed for survival assays and qRT-PCR results. Animal infection data were analyzed using the non-parametric Mann–Whitney U-test. Survival curves were created by the Kaplan–Meier method using the product limit method and compared by the log-rank (Mantel–Cox) test. Figures show mean values. Statistical significance was established at p < 0.05.
Wang S., Dai J., Meng Q., Han X., Han Y., Zhao Y., Yang D., Ding C, & Yu S. (2014). DotU expression is highly induced during in vivo infection and responsible for virulence and Hcp1 secretion in avian pathogenic Escherichia coli. Frontiers in Microbiology, 5, 588.
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