Abi 7500 real time thermal cycler

Manufactured by Thermo Fisher Scientific

Sourced in China, France, United States

The ABI 7500 real-time thermal cycler is a laboratory instrument designed for DNA amplification and quantification. It is capable of performing real-time PCR (polymerase chain reaction) experiments. The core function of the ABI 7500 is to precisely control the temperature and cycling of samples during the PCR process.

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Lab products found in correlation

Taqman assay, by Thermo Fisher Scientific (4 mentions) Rneasy mini kit, by Qiagen (2 mentions) Rt2 first strand kit, by Qiagen (2 mentions) Sybr green rox master mix, by Qiagen (2 mentions) Rt2 profiler pcr array mouse oxidative stress and antioxidant defense, by Qiagen (2 mentions) Goldenstar rt6 cdna synthesis mix, by Tsingke (1 mentions) Primer express 3, by Thermo Fisher Scientific (1 mentions) Superscript 3 rt kit, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Primescript rt reagent kit with gdna eraser, by Takara Bio (1 mentions) Rna extraction kit, by Tiangen Biotech (1 mentions) Taqman assaysfrom, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

ABI 7500 (1035 citations) Thermal cycler (242 citations) ABI 7500 thermal cycler (88 citations) ABI 7500 cycler (27 citations) 7500 thermal cycler (19 citations) 7500 real-time cycler (14 citations) ABI thermal cycler (12 citations) 7500 Real-time Thermal cycler (6 citations) ABI 7500 real-time cycler (6 citations) ABI 7500 Fast Real-Time PCR Thermal cycler (3 citations)

10 protocols using abi 7500 real time thermal cycler

Transcriptome Analysis of A. alternata Mutants

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For RNA-seq analyses, total RNA isolated from conidial suspensions of WT, ∆AaSho1 and ∆AaSho1-C mutant strains cultured on Gelbond PAG film coated with fruit wax for 6 h. The whole genome of A. alternata was download form NCBI (https://www.ncbi.nlm.nih.gov/datasets/taxonomy/5599/). The sequencing and transcriptome analyses were based on those of Li et al. [30] (link) and Gai et al. [31] (link). For each treatment, three independent replicates were performed.
For qRT-PCR, the total RNA was collected through the TRIzol Universal Reagent (Accurate Biology, Hunan, China), and the First-strand cDNA was obtained by the Goldenstar™ RT6 cDNA Synthesis Mix (Tsingke Biotechnology, Beijing, China). qRT-PCR was performed on the ABI7500 real-time thermal cycler (Applied Biosystems) using SYBR^Ⓡ Premix Ex TaqTM II (Tli RNaseH Plus) ROX plus (Takara, Dalian, China). The methods used to process the data are described in Qin et al. [32] (link). The qRT-PCR primers were listed in Table S1. For each treatment, three independent replicates were performed.

Liu Y., Yuan J., Li Y., Bi Y, & Prusky D.B. (2024). The sensor protein AaSho1 regulates infection structures differentiation, osmotic stress tolerance and virulence via MAPK module AaSte11-AaPbs2-AaHog1 in Alternaria alternata. Computational and Structural Biotechnology Journal, 23, 1594-1607.

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Quantifying Oxidative Stress Responses in Macrophages

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Total RNA was purified from samples using RNA kit (Thermo-Fisher) following the manufacturer’s protocol. RNA was further purified by RNeasy mini kit (Qiagen, Valencia, CA). The cDNA was obtained using RT2 First-strand kits (Qiagen) following the manufacturer’s instructions. PCR reactions were performed in an ABI 7500 real-time thermal cycler (Applied Biosystems), run by Melt High Resolution (HRM) software, using SyBrGreen ROX Mastermix (Qiagen). The changes in genes involved in the oxidative stress response were measured in macrophages isolated from BAT, and stimulated with Meth or NE, as above, using RT² Profiler™ PCR Array Mouse Oxidative Stress and Antioxidant Defense (Qiagen, Cat No. 330231). Primers in Table 1 were purchased from Qiagen. The expression was normalized by an average of GAPDH and 18S expression. Results were expressed as log 2 ddCT relative values.

Sanchez-Alavez M., Bortell N., Basova L., Samad F, & Marcondes M.C. (2020). Macrophages and brown adipocytes cross-communicate to modulate a thermogenic program following methamphetamine exposure. International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 37(1), 1368-1382.

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Quantifying Hsp60 and TH Expression in Parkinson's Disease

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The Hsp60 and TH primers for quantitative PCR were designed by primer express 3.0 (Applied Biosystems, Darmstadt, Germany) and their specificity was confirmed by blastn analysis. Their sequences were as followed: Hsp60_fwd: 5′-GCC GCC CCG CAG AA-3′, Hsp60_rev: 5′-CCT GGA CAC CGG TCT CAT CT-3′, TH_fwd: 5′-GCA CCT TCG CGC AGT TCT-3′, TH_rev: 5′-ACA GCG TGG ACA GCT TCT CA-3′. Mesencephalon ss PD patients and age-matched control subjects (n = 5 per group) were provided by the INSERM UMR_S1127 brain bank. The SN was dissected from frozen slides. Total RNA was extracted with Trizol (Invitrogen, Cergy Pontoise Cedex, France) and the quantity and quality were determined by Nanodrop and Agilent, respectively. One microgram of total RNA was reverse-transcribed with Superscript III RT-kit (Invitrogen, Cergy Pontoise Cedex, France) according to the manufacturer’s instructions. qPCR was performed with SYBR GreenER TM qPCR SuperMix kit (Invitrogen, Cergy Pontoise Cedex, France) in ABI 7500 real time thermal cycler (Applied Biosystems, Darmstadt, Germany). The relative mRNA expression levels were normalized by the geometric mean of two housekeeping genes (GAPDH and HPRT) with qBaseplus software (Primerdesign, Southampton, UK).

Noelker C., Morel L., Osterloh A., Alvarez-Fischer D., Lescot T., Breloer M., Gold M., Oertel W.H., Henze C., Michel P.P., Dodel R.C., Lu L., Hirsch E.C., Hunot S, & Hartmann A. (2014). Heat shock protein 60: an endogenous inducer of dopaminergic cell death in Parkinson disease. Journal of Neuroinflammation, 11, 86.

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Quantifying Oxidative Stress Responses in Macrophages

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Scallop RNA Extraction and Expression Analysis

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A Tiangen RNA extraction kit (Tiangen, China) was used to isolate total RNA from the scallop tissues. Reverse transcription PCR was performed using a PrimeScript RT reagent kit with gDNA eraser (Takara, Japan). qRT-PCR was performed using SYBR Green 2× Master Mix (Takara) and an ABI 7500 real-time thermal cycler (Applied Biosystems, USA). Primers CfIKK3-qRT-F/R were used to amplify the target gene fragments; qRT-PCR was programmed as follows: 6 min at 95°C, followed by 40 cycles of 10 s at 95°C and 30 s at 60°C. The specificity of the PCR products was analyzed using a melting curve. Relative gene expression levels were evaluated using the 2^–ΔΔCt method (26 (link)). The housekeeping EF-1α gene (GenBank accession number DT716075.1) was used as the internal control gene. The CfIKK3 expression levels following PAMP challenge were normalized to the expression in PBS-treated scallops. Differences were considered significant if the p-value was less than 0.05.

Liu W., Ma J., Chen J., Huang B., Liu F., Li L., Fan N., Li F., Zheng Y., Zhang X., Wang X., Wang X., Wei L., Liu Y., Zhang M., Han Y, & Wang X. (2023). A novel TBK1/IKKϵ is involved in immune response and interacts with MyD88 and MAVS in the scallop Chlamys farreri. Frontiers in Immunology, 13, 1091419.

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Real-Time PCR Gene Expression Analysis

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Gene expression was quantitated by real-time PCR, using TaqMan assays
from Applied Biosystems, an ABI 7500 real-time thermal cycler, and ABI software
(Life Technologies).

Hayakawa K., Formica A.M., Colombo M.J., Shinton S.A., Brill-Dashoff J., Morse HC I.I.I., Li Y.S, & Hardy R.R. (2016). Loss of a chromosomal region with synteny to human 13q14 occurs in mouse chronic lymphocytic leukemia that originates from early-generated B1 B cells. Leukemia, 30(7), 1510-1519.

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Quantifying ZAP70 Expression in FO B and pB1a

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Gene expression was quantitated by real-time PCR, using TaqMan assays from Applied Biosystems, an ABI 7500 real-time thermal cycler, and ABI software (Life Technologies). Relative gene expression levels were normalized using β-actin values for mRNA as a standard.
For spleen FO B versus pB1a in 2 mo, check of increased ZAP70 by anti-IgM, TNFa (aa80-235), DAP (C12-iE-DAP) (from InvivoGen), MDP (from InvivoGene). FO B (1 × 10⁵, 25λ) and pB1a cells (1 × 10⁵, 25λ) and together with anti-mouse IgM (15λ), DAP(15λ), MDP(15λ), TNFa (100 ng/ml, 15λ), then 20 h later, RT-PCR analysis with ZAP70.

Hayakawa K., Zhou Y, & Shinton S.A. (2024). B-1 derived anti-Thy-1 B cells in old aged mice develop lymphoma/leukemia with high expression of CD11b and Hamp2 that different from TCL1 transgenic mice. Immunity & Ageing : I & A, 21, 22.

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Quantitative Real-Time PCR Analysis

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Gene expression was quantitated by real-time PCR, using TaqMan assays from Applied Biosystems, an ABI 7,500 real-time thermal cycler, and ABI software (Life Technologies). Relative gene expression levels were normalized using β-actin values for mRNA and sno202 for miRNA as a standard.

Hayakawa K., Li Y.S., Shinton S.A., Bandi S.R., Formica A.M., Brill-Dashoff J, & Hardy R.R. (2019). Crucial Role of Increased Arid3a at the Pre-B and Immature B Cell Stages for B1a Cell Generation. Frontiers in Immunology, 10, 457.

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Quantitative Real-Time PCR Protocol

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Gene expression was quantitated by real-time PCR, using TaqMan assays from Applied Biosystems, an ABI 7500 real-time thermal cycler, and ABI software (Life Technologies). Relative gene expression levels were normalized using β-actin (and sno202 for miRNA) values as a standard. Primer sets for RT-PCR: IL-10; 5-GACTGGCATGAGGATCAGCA-3 and 5-GGCCTTGTAGACACCTTGGT. Cyclin D1; 5-AGGCAGCGCGCGTCAGCAGCC-3 and 5-TCCATGGCCCGGCCGTCTGGG.

Hayakawa K., Formica A.M., Nakao Y., Ichikawa D., Shinton S.A., Brill-Dashoff J., Smith M.R., Morse HC I.I.I, & Hardy R.R. (2018). Early Generated B-1–Derived B Cells Have the Capacity To Progress To Become Mantle Cell Lymphoma–like Neoplasia in Aged Mice. The Journal of Immunology Author Choice, 201(2), 804-813.

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Quantitative Real-Time PCR Gene Expression

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Hayakawa K., Formica A.M., Brill-Dashoff J., Shinton S.A., Ichikawa D., Zhou Y., Morse HC I.I.I, & Hardy R.R. (2016). Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression. The Journal of Experimental Medicine, 213(13), 3007-3024.

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