Sodium hydrogen carbonate

Manufactured by Fujifilm

Sourced in Japan

Sodium hydrogen carbonate, also known as baking soda, is a chemical compound with the formula NaHCO3. It is a white crystalline solid that is commonly used in various laboratory applications. The core function of sodium hydrogen carbonate is to act as a buffering agent, maintaining a stable pH in aqueous solutions.

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Lab products found in correlation

22 protocols using sodium hydrogen carbonate

Sensitive HPLC Quantification of Colistin and Netilmicin

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Analytical grade colistin sulfate, netilmicin sulfate, 9-fluorenylmethyl chloroformate (FMOC-Cl), trichloroacetic acid, sodium hydroxide, acetone, sodium hydrogen carbonate, and boric acid, and HPLC grade methanol, acetonitrile, tetrahydrofuran, and distilled water were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The serum employed for quality control (QC) was purchased from Alfresa Pharma Corporation (Osaka, Japan).
Stock solutions of colistin sulfate (100 μg/mL) and netilmicin sulfate (5 μg/mL) internal standard were prepared by dissolving 1.0 and 0.05 mg of the respective substances in 10 mL of distilled water. A 100 mM FMOC-Cl stock solution was prepared by dissolving 258.7 mg of FMOC-Cl in 10 mL of acetonitrile. The carbonate buffer (1 wt%, pH 10) was prepared by dissolving the sodium hydrogen carbonate (1 g) in distilled water (100 mL) and the pH of the solution was adjusted to 10 using sodium hydroxide. All solutions were stable for at least 2months when stored in a refrigerator at 4 °C.

Hanai Y., Matsuo K., Kosugi T., Kusano A., Ohashi H., Kimura I., Hirayama S., Nanjo Y., Ishii Y., Sato T., Miyazaki T., Nishizawa K, & Yoshio T. (2018). Rapid, simple, and clinically applicable high-performance liquid chromatography method for clinical determination of plasma colistin concentrations. Journal of Pharmaceutical Health Care and Sciences, 4, 22.

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Synthesis and Characterization of Cyclodextrin Derivatives

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FPB-βCyD, PB-βCyD, and 1 were synthesised according to our previous reports.^{5–7 (link)} Dimethyl sulfoxide (DMSO, Luminasol®, Dojindo Laboratories), sodium chloride (Fujifilm Wako Chemicals), disodium hydrogen phosphate (Fujifilm Wako Chemicals), sodium hydrogen carbonate (Fujifilm Wako Chemicals), sodium carbonate (Fujifilm Wako Chemicals), d-fructose (Fujifilm Wako Chemicals), d-glucose (Fujifilm Wako Chemicals), d-galactose (Fujifilm Wako Chemicals), d-mannose (Fujifilm Wako Chemicals), d-ribose (Fujifilm Wako Chemicals), d-xylose (Fujifilm Wako Chemicals), hydrogen chloride aq. (Fujifilm Wako Chemicals), 50% sodium hydroxide solution (super special grade, Fujifilm Wako Chemicals), and Milli-Q water were used for spectroscopic measurements.

Sugita K., Suzuki Y., Tsuchido Y., Fujiwara S., Hashimoto T, & Hayashita T. (2022). A simple supramolecular complex of boronic acid-appended β-cyclodextrin and a fluorescent boronic acid-based probe with excellent selectivity for d-glucose in water. RSC Advances, 12(31), 20259-20263.

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Preparation of Complete RPMI Medium

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To prepare complete RPMI medium, RPMI 1640 (10.2 g, Nissui Pharmaceutical Co., Ltd.,
Tokyo, Japan) was dissolved to 1 L of distilled water and sterilized. L(+)-glutamine
(0.03%, FUJIFILM Wako Pure Chemical Corp.), sodium hydrogen carbonate (0.2%, FUJIFILM Wako
Pure Chemical Corp.), penicillin G potassium (100 U/mL, Meiji Seika Pharma, Tokyo, Japan),
streptomycin (100 µg/mL, Meiji Seika Pharma), 2-mercaptoethanol (50 µM, FUJIFILM Wako Pure
Chemical Corp.) were further added (serum-free RPMI). Fetal calf serum (FCS; Gibco,
Carlsbad, CA, USA) was added to the serum-free RPMI to 10% (complete RPMI).

NAKAJIMA-ADACHI H., TAMAI M., NAKANISHI H, & HACHIMURA S. (2022). Extracts of Gluconacetobacter hansenii GK-1 induce Foxp3+T cells in food-allergic mice by an IL-4-dependent or IL-4-independent mechanism. Bioscience of Microbiota, Food and Health, 41(3), 137-144.

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Fabricating Nanoparticles via Chemical Synthesis

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PI, hydrogen peroxide, sulfonic acid, nitric acid, hydrochloric acid, sodium hydrogen carbonate, sodium hydrogen phosphate, sodium dihydrogen phosphate and ammonia water were purchased from WAKO Chemicals (Tokyo, Japan). SYTO 9 and Cy3 were obtained from Thermo Fisher Scientific (Tokyo, Japan). Hydrofluoric acid was obtained from Daikin Industries, Ltd. (Osaka, Japan). Polystyrene (PS) beads (diameter: 200 nm) were purchased from Funakoshi Co., Ltd. (Tokyo, Japan). P-type Si wafer (crystal orientation: 100, diameter: 101.6 mm) was purchased from SUMCO Co. (Tokyo, Japan).

Jindai K., Nakade K., Masuda K., Sagawa T., Kojima H., Shimizu T., Shingubara S, & Ito T. (2020). Adhesion and bactericidal properties of nanostructured surfaces dependent on bacterial motility. RSC Advances, 10(10), 5673-5680.

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Virus Neutralization Test Protocol

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The virus neutralization test was performed according to a method reported previously [33 (link)]. Each 50 μL of serum were serially diluted twofold with 50 μL EMEM supplemented with 3 mg/mL tryptose phosphate broth (Becton Dickinson), 0.292 mg/mL L-glutamine (FUJIFILM Wako Pure Chemical Corporation), 1.125 mg/mL sodium hydrogen carbonate (FUJIFILM Wako Pure Chemical Corporation) on 96-well plates. An equal volume of two representative BVDV strains, Nose (BVDV1) and KZ91-CP (BVDV2), containing 200 TCID₅₀ was added to each well, and incubated at 37 °C for 1 h. Thereafter, 100 μL of BFM cells (approximately 1.5 × 10⁴ cells) were added into all wells, and incubated at 37 °C in 5% CO₂ for 5 days. Appearance of the cytopathogenic effect (CPE) was observed using a microscope (Olympus Corporation, Tokyo, Japan). The virus neutralization titer for each serum was expressed as the reciprocal of the highest dilution that inhibited CPE.

Goto Y., Yaegashi G., Fukunari K, & Suzuki T. (2021). Clinical Analysis for Long-Term Sporadic Bovine Viral Diarrhea Transmitted by Calves with an Acute Infection of Bovine Viral Diarrhea Virus 2. Viruses, 13(4), 621.

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Characterization of Influenza Virus Hemagglutinin Interactions

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11-Bromoundecane-1-thiol was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Sodium hydrogen carbonate, magnesium sulfate, gelatin, ethyl acetate (EA), n-hexance, N,N-dimethylformamide (DMF) and dichloromethane (DCM) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). N-Hydroxyphthalimide, 6′-sialyllactose sodium salt and 3′-sialyllactose sodium salt were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). All chemicals and reagents were used as received. Influenza A H1N1 (A/California/04/2009) hemagglutinin and influenza A virus subtype H5N1 (A/Vietnam/1194/2004) hemagglutinin were purchased from Sino Biological Inc. (Beijing, China). Though influenza A/Vietnam/1194/2004 was isolated from human, the specific interaction between HA and avian SA have been confirmed at previous report (Xiong et al., 2013 (link)). Human influenza A virus subtype H1N1 (A/PR/8/34) was cultivated in chicken embryos and then detoxified using 0.05% formalin solution. The size distribution of H1N1 influenza A virus particles in solution was measured using a qNano nanoparticle analyzer from Izon Science Ltd., Christchurch, New Zealand. Zeta potential measurement was performed using a Zetasizer Nano ZS from Malvern Instruments Ltd., (Worcestershire, UK).

Horiguchi Y., Goda T., Matsumoto A., Takeuchi H., Yamaoka S, & Miyahara Y. (2017). Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors. Biosensors & Bioelectronics, 92, 234-240.

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Soft Agar Colony Formation Assay

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For soft agar colony formation assay, 2X MEM was prepared using 10X MEM (cat. no. M0275; MilliporeSigma), FBS, sodium hydrogen carbonate (FUJIFILM Wako Pure Chemical Corporation), GlutaMax (Thermo Fisher Scientific. Inc.), sodium pyruvate and penicillin-streptomycin (Thermo Fisher Scientific, Inc.). Cells were transfected with miR-10b-5p or NC mimic in 35-mm dishes (50,000 cells/dish). Subsequently, 1.6 and 0.6% agar solutions were prepared using agar powder (FUJIFILM Wako Pure Chemical Corporation) diluted with PBS. Prewarmed 2X MEM and melted 1.6% agar solution were mixed (1:1 ratio) and transferred into six-well plates to form the bottom agar layer. Then, a total of 24 h post-transfection, the cells were trypsinized and resuspended in a prewarmed 2X MEM. The cell suspension and melted 0.6% agar solution were mixed (1:1 ratio) and placed on the bottom agar layer (3,000 cells/well). The cells were incubated with culture medium for 14 days in a humidified incubator at 37°C with 5% CO₂. Then, cells were stained with 0.01% crystal violet for 1 h at room temperature, and the colonies (>50 cells) were manually counted. Images were captured using a WRAYCAM-NF300 light microscope (WRAYMER Inc.).

Yoshida K., Yokoi A., Kitagawa M., Sugiyama M., Yamamoto T., Nakayama J., Yoshida H., Kato T., Kajiyama H, & Yamamoto Y. (2023). Downregulation of miR‑10b‑5p facilitates the proliferation of uterine leiomyosarcoma cells: A microRNA sequencing‑based approach. Oncology Reports, 49(5), 86.

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Nanoscale Surface Characterization Protocol

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Propidium iodide (PI), hydrogen peroxide, sulfuric acid, nitric acid, hydrochloric acid, sodium hydrogen carbonate, sodium hydrogen phosphate, sodium dihydrogen phosphate, magnesium chloride, acetone and ammonia water were purchased from Fujifilm-Wako Chemicals (Tokyo, Japan). SYTO 9 was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Hydrofluoric acid was obtained from Daikin Industries, Ltd (Osaka, Japan). 1,1,1,3,3,3-Hexamethyldisilazane (HMDS) was purchased from Shin-Etsu Chemical Co., Ltd (Tokyo, Japan). Polystyrene (PS) beads (diameter: 200 nm) were purchased from Funakoshi Co., Ltd (Tokyo, Japan). A p-type Si wafer (crystal orientation: 100, diameter: 101.6 mm) was purchased from SUMCO Co (Tokyo, Japan).

Mimura S., Shimizu T., Shingubara S., Iwaki H, & Ito T. (2022). Bactericidal effect of nanostructures via lytic transglycosylases of Escherichia coli. RSC Advances, 12(3), 1645-1652.

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Peptide Nanogel Biodegradability Evaluation

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The peptide nanogel biodegradability in enzyme-containing buffer solution was investigated. The peptide nanogel solution (10 mL) was added to 10 mL 0.02 mol L⁻¹ sodium hydrogen carbonate (guaranteed reagent grade, Fujifilm Wako Pure Chemical), 1 mmol L⁻¹ calcium chloride (90% purity, Fuji-film Wako Pure Chemical), and 0.01 wt.% protease (from Aspergillus oryzae, Tokyo Chemical Industry, Tokyo, Japan). The mixed solutions were incubated at 37 °C for 6 days. Then, the absorbance of the treated solutions was measured using a spectrophotometer (U-3310 spectrophotometer, Hitachi, Tokyo, Japan). The degradation rate was determined from the absorbance at 273 nm using Equation (1):
where Abs₁ and Abs₀ are the absorbance values of the filtrate and residue, respectively.

Kimura A., Arai T., Ueno M., Oyama K., Yu H., Yamashita S., Otome Y, & Taguchi M. (2022). Synthesis of Small Peptide Nanogels Using Radiation Crosslinking as a Platform for Nano-Imaging Agents for Pancreatic Cancer Diagnosis. Pharmaceutics, 14(11), 2400.

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Culturing U-87 GFP Brain Tumor Cells

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U-87-green fluorescent protein (GFP) cells were used as culture cell lines. U-87 cells are human brain tumor cancer cells that express GFP. The medium was prepared by mixing Dulbecco’s modified eagle’s medium (DMEM, 12800-017, Thermo Fisher Scientific Inc., Waltham, MA, USA) with 10 v/v% fetal bovine serum (FBS, Merck KGaA, Darmstadt, Germany), 1 v/v% penicillin (P4333, Merck KGaA, Darmstadt, Germany), 2.5 v/v% HEPES buffer (1M, GB10, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 3.7 g Sodium hydrogen carbonate (191-01305, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 0.1 v/v% Amphotericin B (15290018, Thermo Fisher Scientific Inc., Waltham, MA, USA), and 0.4% G418 (10131035, Thermo Fisher Scientific Inc., Waltham, MA, USA). U-87 cells were cultured in a 5% CO₂ atmosphere at 37 °C. Phosphate-buffered saline (PBS, T900, Takara Bio Inc., Shiga, Japan) was used as the cell-washing solution. A 0.11% Trypsin/1 mM EDTA solution (25200056, Thermo Fisher Scientific Inc., Waltham, MA, USA) was used to remove the cells adhered to the bottom of the cell culture dish, which had a diameter of ϕ90 mm (S90-NC18; Fine Plus International Ltd., Kyoto, Japan).

Ueno H, & Yamamura S. (2024). Fabrication Method for Shape-Controlled 3D Tissue Using High-Porosity Porous Structure. Bioengineering, 11(2), 160.

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