Mtt cell proliferation assay kit

Cell proliferation was assessed by MTT assay using the MTT Cell Proliferation Assay Kit (Trevigen, Gaithersburg, MD, USA). Cells were seeded into 96-well plates at a density of 2 × 10⁶/well, and each group had five replicates. MTT was measured at 24, 48, 72, 96, and 120 h to measure the proliferation ability of the transfected cells.

A Trevigen tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) cell proliferation assay kit was used to assess cell viability according to the manufacturer’s instructions (Trevigen, Gaithersburg, MD, USA). In proliferation and cytotoxicity assays, MTT is used to determine cell viability. Cells were seeded into 96-well microplates at a density of 2,000 cells/100 mL of culture medium. After seeding, the cells were treated for 24, 48–72 h with dimethyl sulfoxide (DMSO) as a control or with different doses of drugs. A microplate reader (Spectral Max250; Molecular Devices, Sunnyvale, CA, USA) was used to measure the optical density at 570 nm after the cells were incubated for 4 h in medium containing MTT and lysed with DMSO.

The cell viability was assessed using standard MTT assay according to the manufacturer’s instructions (MTT cell proliferation assay kit, purchased from TREVIGEN, catalog #4890-025-K, 8405 Helgerman Ct, Gaithersburg, MA, USA).
Analysis of cell proliferation was performed in the presence of different concentration of any synthetized compounds on cell lines seeded in 96-well plates at the density of 1–2 × 10³ cells/well in serum-containing media. After 24 h of incubation at 37 °C, cells were treated with increasing concentrations of compounds or vehicle (DMSO; maximum concentration ≤0.5%), following which the cells were cultured in drugs for 48 h, and 10 µL MTT solution (TREVIGEN, Helgerman Ct) was added subsequent to drawing off the medium. The mixture was incubated for an additional 4 h at 37 °C, the absorbance was read at 572 nm in a microplate reader (Biocompare, Italy). Cell viability was expressed as the percentage of proliferated cells compared to cells treated with the different compounds. The same cells were used as control. All experiments were repeated in triplicate [38 (link),39 (link),40 (link),41 (link)].

MTT cell proliferation assay kit (Trevigen, Gaithersburg, MD, USA) was used to assess the cell viability according to Verma et al. (2010 (link)) with slight modification. The bMECs at a density of 1 × 10⁵ cells per well were cultured in 96-well plates. After being treated as mentioned above, the cells were washed three times with PBS (pH 7.2), and cells were incubated with 100 μL medium and 10 μL of the activated MTT solution at 37°C for 4 h, after that treated with 50 μL DMSO (Sigma-Aldrich), and the absorption was measured by microplate reader (SpectraMax 190, Molecular Devices Corporation, Sunnyvale, CA, USA) at 570 nm. Cell viability was determined as the percentages (%) of the control.

Cell viability was assessed with MTT cell proliferation assay kit (Trevigen Inc., Gaithersburg, MD) according to manufacturer’s instructions. In brief, HT22 cells were plated into 96-well plates (5000/well, Corning Inc., New York, NY). After seeding 24 h the cultures exposed to various concentrations of glutamate (2, 4, 6, 8, and 10 mM). After 24 h of incubation, the results were read in the SpectraMax microplate reader (Molecular Devices, Sunnyvale, CA). Glutamate of 6 mM was selected for the subsequent experiments.