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Axiovert camera

Manufactured by Zeiss
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The Axiovert camera is a high-performance microscope camera designed for various imaging applications. It features a large sensor size and high resolution, enabling the capture of detailed and accurate images. The camera is intended for use with Zeiss microscopes, providing seamless integration and optimized performance.

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Lab products found in correlation

Lsm 700 confocal microscope, by Zeiss (4 mentions) Phalloidin, by Cell Signaling Technology (1 mentions) Cd45 30 f11, by BD (1 mentions) Cd68 kp1, by Abcam (1 mentions) α sma 1a4, by Abcam (1 mentions) Dectin 1, by Abcam (1 mentions) Cd11b m1 70, by BioLegend (1 mentions) M csf, by Abcam (1 mentions) Cd3 17a2, by BioLegend (1 mentions) Goat anti rabbit igg fitc, by Santa Cruz Biotechnology (1 mentions) Dapi counterstain, by Vector Laboratories (1 mentions) P syk, by Abcam (1 mentions) Axioscope 40 microscope camera system, by Zeiss (1 mentions) Donkey anti goat igg pe, by Santa Cruz Biotechnology (1 mentions) Cd45 microbeads, by Miltenyi Biotec (1 mentions)

2 protocols using axiovert camera

1

Liver Tissue Histopathology and Immunohistochemistry

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Liver tissues were fixed overnight in 10% formaldehyde and were embedded in paraffin. Slides were stained with H&E, Masson's Trichrome, or Picric acid-Sirius red. For immunohistochemical analysis, paraffin-embedded or frozen sections were incubated with antibodies against mouse CD45 (30-F11; BD Bioscience), CD68 (KP1; Abcam), MPO (Rabbit polyclonal; Abcam), Dectin-1 (R1-8g7; Invivogen), PCNA (PC10; Biolegend), TLR4 (Rabbit polyclonal; Abcam), α-SMA (1A4; Abcam), Phalloidin (Cell Signaling), or M-CSF (Rabbit polyclonal; Abcam). Human liver sections were stained with an antibody against Dectin-1 (Rabbit polyclonal; Abcam) or CD11b (M1/70; Biolegend). Quantification was performed by examining 10 high powered fields (HPFs) per slide. Fibrosis was quantified based on Trichrome staining using a computerized grid as described (Ochi et al., 2012a (link)). Immunofluorescent imaging was performed using a LSM 700 confocal microscope and an Axiovert camera (Zeiss).
Seifert L., Deutsch M., Alothman S., Alqunaibit D., Werba G., Pansari M., Pergamo M., Ochi A., Torres-Hernandez A., Levie E., Tippens D., Greco S.H., Tiwari S., Ly N.N., Eisenthal A., van Heerden E., Avanzi A., Barilla R., Zambirinis C.P., Rendon M., Daley D., Pachter H.L., Hajdu C, & Miller G. (2015). Dectin-1 Regulates Hepatic Fibrosis and Hepatocarcinogenesis by Suppressing TLR4 Signaling Pathways. Cell reports, 13(9), 1909-1921.
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2

Histological and Immunohistochemical Analysis of Liver

Cited 1 time
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For histological analysis, liver specimens were fixed with 10% buffered formalin, dehydrated in ethanol, and then embedded with paraffin and stained with hematoxylin-eosin (H&E) or Masson trichrome. Percent cell death was determined on a computerized grid as we have described10 (link). For immunohistochemical analysis, slides were stained for anti-mouse CD45 (30-F11; BD Biosciences), MPO (polyclonal, Abcam), CD3 (17A2; Biolegend), CD68 (pAB; Abcam), p-Syk (polyclonal, Abcam), as well as anti-human Mincle (AT16E3, Abcam), SAP-130 (polyclonal, Abcam), and p-Syk (polyclonal, Abam). TUNEL staining was performed using a kit (EMD Millipore, Billerica, MA). For immunofluorescent imaging, murine CD45+ NPCs were isolated using CD45 MicroBeads (Miltenyi, Bergisch Gladbach, Germany) stained for Mincle (CLEC-4E polyclonal, Santa Cruz Biotechnology, Dallas, TX) or p-Syk (polyclonal, Abcam), and then detected with donkey anti-goat IgG-PE, goat anti-rabbit IgG-Fitc (both Santa Cruz Biotechnology), and DAPI counterstain (Vector Laboratories, Burlingame, CA). Light microscopic images were captured with a Zeiss Axioscope 40 microscope/camera system (Zeiss, Thornwood, NY). Immunofluorescent imaging was performed using a LSM 700 confocal microscope and an Axiovert camera (Zeiss). Data was quantified by examining 10 high powered fields (HPFs) per slide.
Greco S.H., Torres-Hernandez A., Kalabin A., Whiteman C., Rokosh R., Ravirala S., Ochi A., Gutierrez J., Salyana M.A., Mani V.R., Nagaraj S.V., Deutsch M., Seifert L., Daley D., Barilla R., Hundeyin M., Nikifrov Y., Tejada K., Gelb B.E., Katz S.C, & Miller G. (2016). Mincle Signaling Promotes Con-A Hepatitis. Journal of immunology (Baltimore, Md. : 1950), 197(7), 2816-2827.
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