Pe conjugated anti mouse cd8a

PLGA (Resomer RG502H, monomer ratio 50:50, molecular weight [MW] 10–12 kDa) was purchased from Boehringer Ingelheim (Ingelheim, Germany). Polyvinyl alcohol (80% hydrolyzed, MW 9–10 kDa), chicken egg OVA, and poly I:C sodium salt were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Fluorescein isothiocyanate (FITC)-conjugated antimouse interferon (IFN)-γ, R-phycoerythrin (PE)-conjugated antimouse CD40, OVA-specific (SIINFEKL/H-2Kb) antibody, and mouse TNF-α, IL-1β, IL-6, IL-12p70 enzyme-linked immunosorbent assay (ELISA) Ready-SET-Go kit were purchased from eBioscience (San Diego, CA, USA). FITC-conjugated antimouse CD11c antibody, PE-conjugated antimouse CD8a, CD80, CD86, MHC class I, MHC class II, and mouse IFN-β ELISA kit were purchased from Biolegend (San Diego, CA, USA). Roswell Park Memorial Institute Medium (RPMI) 1640 and fetal bovine serum (FBS) were purchased from Biowest (Nuaille, France). Granulocyte-macrophage colony-stimulating factor was purchased from JW CreaGene (Gyeonggi, South Korea). E7 peptide (MW 2.4 kDa, 42–64 amino acids, AGQAEPDRAHYNIVTFCCKCDS) was purchased from AnyGen Co. (Seoul, South Korea). Anti-CD8 antibody for CD8⁺ T cell depletion was purchased from BioXcell (West Lebanon, USA). All other materials were of analytical grade and used without further purification.