Insulin elisa kit

Manufactured by Mercodia

Sourced in Sweden, United States

The Insulin ELISA kit is a laboratory assay used to quantify insulin levels in biological samples. It employs the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure the concentration of insulin in the tested sample.

Automatically generated - may contain errors

Lab products found in correlation

Bovine serum albumin (bsa), by Merck Group (5 mentions) Abx pentra 400, by Horiba (3 mentions) Streptozotocin (stz), by Merck Group (3 mentions) Bca protein assay kit, by Thermo Fisher Scientific (3 mentions) Glucagon elisa kit, by Mercodia (2 mentions) 58500 bp recorder, by Ugo Basile (2 mentions) Au2700, by Olympus (2 mentions) Phospho akt at ser473, by Cell Signaling Technology (2 mentions) Cmld 2, by Merck Group (2 mentions) Mg132, by Merck Group (2 mentions) Actinomycin d, by Merck Group (2 mentions) Rodent glucagon elisa kit, by Mercodia (2 mentions) Glucose meter, by Roche (2 mentions) Glp 1 active elisa kit, by Merck Group (2 mentions) Palmitic acid, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Accu chek aviva meter, by Roche (1 mentions) Vacutainer tube, by BD (1 mentions) Hscrp elisa kit, by BioVendor (1 mentions)

Close spelling variants of this product

Mouse insulin ELISA kit (161 citations) Rat insulin ELISA kit (136 citations) Insulin (96 citations) Insulin ELISA (62 citations) Ultrasensitive Rat Insulin ELISA kit (39 citations) Ultrasensitive Mouse Insulin ELISA kit (39 citations) Porcine insulin ELISA kit (16 citations) Ultrasensitive Insulin ELISA kit (14 citations) High Range Rat Insulin ELISA kit (11 citations) ELISAs (9 citations)

96 protocols using insulin elisa kit

Islet Insulin Secretion Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols

After 24 h of culture, 10 size-matched islets were incubated with 2.8 mM glucose Krebs–Ringer buffer (KRB) adjusted to pH 7.3 with HEPES for 60 min at 37 °C. After incubated in low-glucose KRB for 60 min, the islets were stimulated in KRB containing 2.8 mM or 16.7 mM glucose with different concentrations of inhibitors or with vehicle for additional 60 min at 37 °C. An insulin ELISA kit from Mercodia (Uppsala, Sweden) was used to measure the secreted insulin. The results were normalized to the islet total insulin content.
Fifty handpicked islets were placed into perfusion chambers (Swinnex 13; Millipore, MA). At the stabilization period, islets were perfused with 2.8 mM KRB at a speed of 1.0 mL/min for 20 min at 37 °C. Then, islets were perfused with 16.7 mM KRB for 40 min followed by KRB containing the inhibitors. We collected the droplets every 1 min. At the end of the perfusion assay, the islets were collected to assess the insulin content. An insulin ELISA kit from Mercodia (Sweden) was used to detect the secreted insulin. The results were normalized to the islet total insulin content.

Lu J., Zhao R.X., Xiong F.R., Zhu J.J., Shi T.T., Zhang Y.C., Peng G.X, & Yang J.K. (2024). All–potassium channel CRISPR screening reveals a lysine-specific pathway of insulin secretion. Molecular Metabolism, 80, 101885.

+ Open protocol

+ Expand

Insulin Secretion Assay in Mouse and Human Islets

Check if the same lab product or an alternative is used in the 5 most similar protocols

β-Min6 cells (passages 9–12) were seeded at a density of 4×10⁶ cells/well in a 12-well plate for 24 h at 37 °C in an atmosphere of 5% CO₂. Human islets were separated into 60 cm dishes containing 50 islets/dish. Cells were washed three times with glucose-free Krebs buffer, and then incubated in 0.05% BSA Krebs buffer (1 mmol/l glucose) for 1 h at 37 °C in an atmosphere of 5% CO₂. Cells were again washed three times with glucose-free Krebs buffer. Afterwards, cells were cultured in 0.05% BSA Krebs buffer (1 or 25 mmol/l glucose) and treated with vehicle, IBMX, RES, or CUR at the indicated doses for 2 h. Supernatants were collected for insulin measurements using the Ultra-Sensitive Mouse Insulin ELISA Kit (Crystal Chemical, Inc., Downers Grove, IL, USA) for mouse or the Mercodia Insulin ELISA Kit (Mercodia, Winston Salem, NC, USA) for human cells, and performed according to the manufacturer's instructions.

Rouse M., Younès A, & Egan J.M. (2014). Resveratrol and curcumin enhance pancreatic β-cell function by inhibiting phosphodiesterase activity. The Journal of Endocrinology, 223(2), 107-117.

+ Open protocol

+ Expand

Quantification of Insulin Secretion in sc-Beta Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Krebs Ringer buffer (KRB) was prepared by addition of 129 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl₂, 1.2 mM MgSO₄, 1 mM Na₂HPO₄, 1.2 mM KH₂PO₄, 5 mM NaHCO₃, 10 mM HEPES and 0.1% BSA in deionized water and was sterilized with a 0.22 μm filter. The 2 mM glucose solutions were prepared in KRB for low glucose challenge of sc-beta cell clusters. Then, 10–20 sc-beta cell clusters (~5 × 10⁵ cells) were collected from WT, KO, and R138X and pre-incubated in 500 μL 2 mM glucose solution for 1 h. Clusters were then washed once with 2 mM glucose solution and subsequently incubated in 200 µL of 2 mM glucose for 1 h. Finally, 130 μL supernatant from each condition was collected. Cell clusters were centrifuged down and resuspended in 50 µL high salt buffer and sonicated for protein content preparation and DNA measurement with Nano Drop Spectrophotometer ND-1000. Protein content and supernatants in 2 mM glucose solution were processed using Mercodia Insulin ELISA kit (Cat. No. 10-1113-01, Mercodia, Uppsala, Sweden).

Sui L., Du Q., Romer A., Su Q., Chabosseau P.L., Xin Y., Kim J., Kleiner S., Rutter G.A, & Egli D. (2023). ZnT8 Loss of Function Mutation Increases Resistance of Human Embryonic Stem Cell-Derived Beta Cells to Apoptosis in Low Zinc Condition. Cells, 12(6), 903.

+ Open protocol

+ Expand

Insulin Secretion from β-Cells and Islets

Check if the same lab product or an alternative is used in the 5 most similar protocols

β-Min6 cells (passage 9-12) were seeded at 4×10⁶ cells per well in a 12-well plate for 24 hrs at 37°C, 5% CO₂. Human islets were separated into 60 cm dishes containing 50 islets per dish. Cells were washed three times with glucose-free KREBS buffer, and then incubated in 0.05% BSA KREBS buffer (1 mmol L^-1 glucose) for 1 hr at 37°C, 5% CO₂. Cells were again washed three times with glucose-free KREBS buffer. Afterwards, cells were cultured in 0.05% BSA KREBS buffer (1 or 25 mmol L^-1 glucose), and treated with vehicle, IBMX, RES, or CUR at the indicated doses for 2 hrs. Supernatants were collected for insulin measurements using an Ultra-Sensitive Mouse Insulin ELISA kit (Crystal Chemical Inc., Downers Grove, IL) for mouse or Mercodia Insulin ELISA kit (Mercodia, Winston Salem, NC) for human, and performed according to the manufacturer's instructions.

Rouse M., Younès A, & Egan J.M. (2014). Resveratrol and curcumin enhance pancreatic β-cell function by inhibiting phosphodiesterase activity. The Journal of Endocrinology, 223(2), 107-117.

+ Open protocol

+ Expand

Insulin Release from Islet Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Insulin Response tests were performed after coating procedure described above. Naked (control) or Treg cells coated islets were exposed to high (28 mM) and then to low (2.8 mM) glucose in Krebs-Ringer buffer (KRB) solution for 1 h at 37°C. The solutions of each glucose concentration were collected, concentration of released by islets insulin in each was determined by Enzyme-Linked Immunosorbent Assay (ELISA) using Mercodia Insulin ELISA kit (Mercodia AB, Uppsala, Sweden).

Gołąb K., Kizilel S., Bal T., Hara M., Zielinski M., Grose R., Savari O., Wang X.J., Wang L.J., Tibudan M., Krzystyniak A., Marek-Trzonkowska N., Millis J.M., Trzonkowski P, & Witkowski P. (2014). Improved Coating of Pancreatic Islets with Regulatory T cells (Tregs) to Create Local Immunosuppression by Using Biotin-Polyethylene glycol-Succinimidyl Valeric Acid Ester Molecule. Transplantation proceedings, 46(6), 1967-1971.

+ Open protocol

+ Expand

Fasting Glucose, Insulin, and Sensitivity Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

The concentration of fasting plasma glucose (FPG) was measured by using the Accu-Chek Aviva meter (Roche, Shanghai, China). The concentration of fasting insulin (FINS) was determined by using the Mercodia Insulin ELISA Kit (cat. no. 10-1113-01; Mercodia, Uppsala, Sweden) according to the manufacturer’s procedure. Insulin sensitivity index (ISI) was calculated: ISI = 1/FPG (mmol·L^-1) x FINS (mU·L^-1). Homeostasis model assessment was used to calculate insulin resistance index (IRI): IRI = [FPG (mmol·L^-1) x FINS (mU·L^-1)]/22.5.

Xing Y., Zhang J., Wei H., Zhang H., Guan Y., Wang X, & Tong X. (2019). Reduction of the PI3K/Akt related signaling activities in skeletal muscle tissues involves insulin resistance in intrauterine growth restriction rats with catch-up growth. PLoS ONE, 14(5), e0216665.

+ Open protocol

+ Expand

Fasting Serum Biomarker Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fasting serum samples were collected by low-speed centrifugation of serum separator Vacutainer™ tubes (Becton Dickinson, Franklin Lakes, NJ, USA) that were left to clot for 30 min. All samples were then either analysed immediately or stored at −80 °C prior to analysis. Fasting serum triglyceride, cholesterol and glucose were determined by Pathwest Laboratories using their routine automated procedures on an Architect c1600 analyser. Serum triglyceride, total cholesterol, HDL cholesterol and glucose concentration were measured by using specific enzyme-based colorimetric reagents (Abbott Diagnostics, Abbott Laboratories, Abbott Park, USA; CV <2 %). LDL cholesterol was estimated by using a modified version of Friedewald formula [30 (link)]. Non-HDL cholesterol concentration was calculated as the difference between serum total cholesterol and HDL cholesterol concentration. Insulin level was determined by using the Mercodia insulin ELISA kit (Mercodia AB, Uppsala, Sweden) according to the manufacturer’s instructions. Insulin resistance was assessed by calculation of HOMA-IR score [31 (link)].

Irawati D., Mamo J.C., Dhaliwal S.S., Soares M.J., Slivkoff-Clark K.M, & James A.P. (2016). Plasma triglyceride and high density lipoprotein cholesterol are poor surrogate markers of pro-atherogenic chylomicron remnant homeostasis in subjects with the metabolic syndrome. Lipids in Health and Disease, 15, 169.

+ Open protocol

+ Expand

Biochemical Analysis of Plasma Glucose, Insulin, and Lipids

Check if the same lab product or an alternative is used in the 5 most similar protocols

The concentration of plasma glucose was measured by the glucose oxidase method using an analyzer (Quik-Lab, Ames, IA, USA; Miles Inc., Elkhart, IN, USA). The insulin level was examined using a Mercodia insulin ELISA kit (Mercodia AB, Uppsala, Sweden). Total lipids in liver were extracted according to the previous method [11 (link)]. The levels of lipids were determined using a Fuji Dri-Chem Slide TCHO for cholesterol and a Slide TG for triglyceride to read in a machine of Fuji Dri-Chem 400i (Fujifilm, Tokyo, Japan). In addition, the concentration of BER was estimated using a commercially available ELISA kit (Peninsula Laboratories, Belmont, CA, USA).

Hsu C.C., Lin M.H., Cheng J.T, & Wu M.C. (2017). Diosmin, a Citrus Nutrient, Activates Imidazoline Receptors to Alleviate Blood Glucose and Lipids in Type 1-Like Diabetic Rats. Nutrients, 9(7), 684.

+ Open protocol

+ Expand

Plasma Insulin, IL-6, and Adiponectin Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fasting plasma insulin levels were measured using a Mercodia Insulin ELISA kit (Mercodia, Sweden) according to the manufacturer's instructions. Analysis of plasma IL‐6 and adiponectin was determined using Mouse IL‐6 and Adiponectin Quantikine^® ELISA Kits (both from R&D Systems, Abingdon, UK), respectively, according to the manufacturer's instructions.

Lipina C., Vaanholt L.M., Davidova A., Mitchell S.E., Storey‐Gordon E., Hambly C., Irving A.J., Speakman J.R, & Hundal H.S. (2016). CB1 receptor blockade counters age‐induced insulin resistance and metabolic dysfunction. Aging Cell, 15(2), 325-335.

+ Open protocol

+ Expand

Metabolic and Inflammatory Biomarkers Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Glucose and lipid profiles were measured using a Siemens Dimension RXL chemistry analyzer (Diamond Diagnostics, Holliston, MA, USA). Hemoglobin A1c (HbA1c) levels were determined using the Variant™ device (BioRad, Hercules, CA, USA). Insulin and high-sensitivity CRP (hsCRP) levels were determined using a Mercodia Insulin ELISA Kit (Mercodia AB, Uppsala, Sweden) and an hsCRP ELISA kit (Biovendor, Asheville, NC, USA), respectively. Plasma levels of inflammatory and metabolic markers were measured using bead-based multiplexing technology on a Bioplex-200 system (BioRad). All of the aforementioned assays were performed according to the manufacturers’ instructions. The Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index was calculated using the following formula: HOMA-IR = (glucose × insulin)/22.5.

Khadir A., Kavalakatt S., Cherian P., Warsame S., Abubaker J.A., Dehbi M, & Tiss A. (2018). Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese. Frontiers in Endocrinology, 9, 16.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!