Maleimido c3 nta

Manufactured by Dojindo Laboratories

Sourced in Japan

Maleimido-C3-NTA is a heterobifunctional crosslinker that contains a maleimide group and a nitrilotriacetic acid (NTA) group. The maleimide group can react with sulfhydryl (thiol) groups on proteins or other biomolecules, while the NTA group can bind to metal ions such as nickel, copper, or cobalt. This allows for the immobilization or labeling of proteins or other biomolecules on surfaces or solid supports.

Automatically generated - may contain errors

Lab products found in correlation

Fluorescein reference standard, by Thermo Fisher Scientific (1 mentions) Escin, by Merck Group (1 mentions) Cyclic amp, by Merck Group (1 mentions) Cyclic gmp, by Merck Group (1 mentions) Dna oligo, by Integrated DNA Technologies (1 mentions) Bst 2.0 dna polymerase, by New England Biolabs (1 mentions)

2 protocols using maleimido c3 nta

Synthesis and Characterization of Biomolecular Probes

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Cyclic AMP, cyclic GMP, the other nucleotides, (3-mercaptopropyl) trimethoxysilane and Escin were purchased from Sigma. Nickel (II) sulfate hexahydrate IBMX, ibudilast, and forskolin were purchased from Fujifilm. Maleimido-C3-NTA was purchased from Dojindo. Potassium methanesulfonate was purchased from Tokyo Chemical Industry. Fluorescein reference standard was purchased from Invitrogen.

Kawata S., Mukai Y., Nishimura Y., Takahashi T, & Saitoh N. (2022). Green fluorescent cAMP indicator of high speed and specificity suitable for neuronal live-cell imaging. Proceedings of the National Academy of Sciences of the United States of America, 119(28), e2122618119.

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Synthesis and Ligation of Functionalized Oligonucleotides

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All chemicals unless otherwise specified were purchased from Sigma-Aldrich and were used as received. DNA oligos were ordered from Integrated DNA Technologies Inc. (Coralville, IA, USA). Bst 2.0 DNA polymerase was purchased from New England BioLabs Inc. (Ipswich, MA, USA). CircLigase II single-stranded DNA (ssDNA) Ligase was purchased from Epicenter (Madison, WI, USA). Aminoallyl-dUTP and Traut's reagent were purchased from ThermoFihser Scientific, Inc. (Pittsburgh, PA, USA). Maleimido-C3-NTA was purchased from Dojindo Molecular Technologies Inc. (Kumamoto, Japan).

Sun W., Ji W., Hu Q., Yu J., Wang C., Qian C., Hochu G, & Gu Z. (2016). Transformable DNA Nanocarriers for Plasma Membrane Targeted Delivery of Cytokine. Biomaterials, 96, 1-10.

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