Pe anti human cd235a glycophorin a antibody

Anti‐MAL polyclonal antibody (reactivity: mouse, rat, dog, human, frog), anti‐ceramide polyclonal antibody, horseradish peroxidase (HRP)‐coupled goat antimouse IgG (H + L) antibody, anti‐His monoclonal antibody and fluorescein isothiocyanate (FITC)‐conjugated goat anti‐rabbit IgG (H + L) were purchased from Abcam (Cambridge, MA, USA). 3‐(4, 5‐dimethylthiazol‐2‐yl)‐5(3‐carboxymethoxyphenyl)‐2‐(4‐sulfopheny)‐2H‐tetrazolium inner salt (MTS) was purchased from Promega Corporation (Madison, WI, USA). Anti‐glutathione S‐transferase (GST) monoclonal antibody was purchased from EARTHOX Life Sciences (Millbrae, CA, USA). Annexin V, Annexin V binding buffer and PE anti‐human CD235a (Glycophorin A) antibody were purchased from BioLegend (San Diego, CA, USA). Fluo‐4 and PKH26 Red Fluorescent Cell Linker Kit were purchased from Sigma (St. Louis, MO, USA). BAPTA‐AM, Protease inhibitor and 2ʹ,7ʹ‐Dichlorofluorescin Diacetate were purchased from Sigma (St. Louis, MO, USA).

The dissected tumor sample was digested into single-cell suspension via 1.5 mg/mL collagenase type II (Gibco, 17101015) and 1.5 mg/mL collagenase type IV (Gibco, 17104019) treatment for 30 min at 37°C. APC anti-human CD326 (EpCAM) Antibody (BioLegend, 369810) and PE anti-human CD235a (Glycophorin A) Antibody (BioLegend, 349106) and 7-AAD viability staining solution (BioLegend, 420404) were used in FACS, and CD235a-CD326+ cells were collected. For this sample, single cells were picked into PCR tubes using mouth pipette.