Total RNA was isolated using an RNeasy Mini Kit (#74104, Qiagen, Hilden, Germany) or an RNeasy Plus Mini Kit (#74134, Qiagen) and cDNA was prepared using a SuperScript III First-Strand synthesis system kit (#51101, Invitrogen, Grand Island, NY, USA). TaqMan primer sets for the KGF (#Hs00940253_m1), miR-31 (#4427975), KI-67 (#Hs04260396_g1), KRT1 (#Hs00196158_m1), KRT10 (#Hs00166289_m1), PCNA (#Hs00427214_g1) and RPLP0 (#4333761F) genes were purchased from Applied Biosystems (Foster City, CA, USA). qRT-PCR was performed using the TaqMan universal PCR master mix (#4304437, Applied Biosystems) and an Applied Biosystems 7500 Fast Real-time PCR system. Relative mRNA expression levels of target genes were normalized to those of the housekeeping gene, RPLP0, and calculated using the comparative ∆∆Ct method, according to the manufacturer’s instructions.
Krt10
Manufactured by Thermo Fisher Scientific
Sourced in United States
KRT10 is a laboratory instrument designed for protein analysis. It is capable of detecting and quantifying specific proteins in biological samples using immunochemical techniques. The core function of KRT10 is to provide accurate and reliable protein measurement data to support research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Rneasy mini kit, by Qiagen (2 mentions)
Superscript 3 first strand synthesis system kit, by Thermo Fisher Scientific (1 mentions)
Proliferating cell nuclear antigen (pcna), by Thermo Fisher Scientific (1 mentions)
7500 fast real time pcr system, by Thermo Fisher Scientific (1 mentions)
Rneasy plus mini kit, by Qiagen (1 mentions)
Taqman universal pcr master mix, by Thermo Fisher Scientific (1 mentions)
Tri reagent, by Merck Group (1 mentions)
Superscript vilo master mix, by Thermo Fisher Scientific (1 mentions)
Abi prism 7900 real time pcr instrument, by Thermo Fisher Scientific (1 mentions)
2 protocols using krt10
1
Quantitative Analysis of Gene Expression
2
Quantitative PCR Analysis of Skin Biopsy
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The skin biopsy specimens of each mouse in TRI Reagent® (Sigma-Aldrich Inc) were homogenized, and chloroform was added. After centrifugation, the aqueous phase was transferred to a new tube and isopropanol was added. After centrifugation, the supernatant was removed and 70% ethanol was added to the RNA pellet. RNeasy Mini Kits (Qiagen, Valencia, CA, USA) were used according to the manufacturer’s protocol to isolate RNA from keratinocytes or the skin biopsies of mice. The RNA was reverse transcribed into cDNA using SuperScript® VILOTM MasterMix according to the manufacturer’s protocol (Life Technologies). The cDNA was analyzed using real-time quantitative-PCR and an ABI prism 7900 real-time PCR instrument (Applied Biosystems, ThermoFisher Scientific) as described previously.20 (link) Primers and probes for GAPDH, IL-4, IL-5, IL-10, IL-12, IL-13, IL-33, transforming growth factor (TGF)-β, FLG, LOR, transglutaminase (TGM)-3, keratin (KRT)-1, KRT-10, and desmoglein (DSG)-1 were purchased from Applied Biosystems and normalized to the GAPDH mRNA levels.
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