Cd3ε 145 2c11

Manufactured by BD

Sourced in United States

CD3ε (145-2C11) is a monoclonal antibody that binds to the CD3 epsilon chain, a component of the T cell receptor complex. This antibody is commonly used in flow cytometry and other immunological applications to identify and study T cells.

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Lab products found in correlation

Cd16 32 clone 93, by BioLegend (1 mentions) Cd24 m1 69, by BD (1 mentions) Rorγt b2d, by Thermo Fisher Scientific (1 mentions) Siglecf e50 2440, by BD (1 mentions) Cd43 s7, by BD (1 mentions) Cd21 7g6, by BD (1 mentions) Ra3 6b2, by Thermo Fisher Scientific (1 mentions) Pharmingen, by BD (1 mentions) Cd127 a7r34, by Thermo Fisher Scientific (1 mentions) Cd44 im7, by BD (1 mentions) Live dead aqua, by Thermo Fisher Scientific (1 mentions) Protein transport inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-CD3ε (145–2C11; (10 citations) Anti-CD3ε (clone 145-2C11) (7 citations) CD3ε (clone 145–2C11, (4 citations) Anti-CD3ε antibody (clone: 145-2C11 (3 citations) APC)-conjugated anti-CD3ε (clone 145-2C11 (2 citations) CD3ε-APC (145–2C11; (2 citations) APC-anti-CD3ε (clone 145-2C11; (2 citations) Anti-CD3ε-FITC (145–2C11; (2 citations)

3 protocols using cd3ε 145 2c11

Comprehensive Murine Immune Cell Profiling

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Antibodies targeting the following murine proteins were purchased from BioLegend (San Diego, CA): PD-L1 (clone 10F.9G2), I-A/I-E (M5/114.15.2), CD45 (30-F11), CD19 (6D5), CD11b (M1/70), PD-1 (29F.1A12), TCRβ chain (H57-597), CD8a (53-6.7), PD-L2 (TY25), CD80 (16-10A1), CD4 (GK1.5), Ly6G (1A8), Ly6C (HK1.4), T-bet (4B10), ICOS (C398.4A), OX40 (OX-86), TCRγ/δ (GL3) and CD16/32 (clone 93). Antibodies targeting the following murine proteins were purchased from BD Biosciences (Franklin Lake, NJ): Siglec F (E50-2440), CD24 (M1/69), and CD3ε (145-2C11). Antibodies targeting the following murine proteins were purchased from eBioscience (Asheville, NC): iNOS (CXNFT), FoxP3 (FJK-16s), Ki67 (SolA15), CD11c (N418), Gata3 (TWAJ), and RORγt (B2D). A polyclonal antibody targeting murine arginase was purchased from R&D Systems. Isotype control antibodies were used according to manufacturer’s instructions in all experiments. Antibodies were conjugated to the following fluorophores: Allophycocyanin (APC), Alexa Fluor 700, APC-cyanine 7 (APC-Cy7), fluorescein isothiocyanate (FITC), phycoerythrin (PE), peridinin-chlorophyll-protein (PerCP)-Cy5.5, PE-eFluor 610, PE-Cy5, PE-Cy7, Brilliant Violet (BV) 421, and BV 650.

Roussey J.A., Viglianti S.P., Teitz-Tennenbaum S., Olszewski M.A, & Osterholzer J.J. (2017). Anti-PD-1 Antibody Treatment Promotes Clearance of Persistent Cryptococcal Lung Infection in Mice. Journal of immunology (Baltimore, Md. : 1950), 199(10), 3535-3546.

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Multiparameter Flow Cytometry Analysis

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Single cell suspensions (10⁶ cells/100 µl) were stained on ice for 1 h with antibodies specific for IgM (Il/41), B220 (RA3-6B2), CD25 (PC61.5), IgD (11-26c), CD93 (AA4.1), CD45.1 (A20), CD45.2 (104), CD23 (B3B4), CD4 (GK1.5), CD8α (53-6.7; all from eBioscience), CD3ε (145-2C11; BD Biosciences), CD43 (S7; BD Biosciences), or CD21 (7G6; BD Biosciences) directly conjugated to FITC, PE, PE-Cy7, Percp-cy5.5, APC, APC-Cy7, or APC-780. Data were collected and analyzed using FlowJo.

Veselits M., Tanaka A., Chen Y., Hamel K., Mandal M., Kandasamy M., Manicassamy B., O’Neill S.K., Wilson P., Sciammas R, & Clark M.R. (2017). Igβ ubiquitination activates PI3K signals required for endosomal sorting. The Journal of Experimental Medicine, 214(12), 3775-3790.

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Phenotypic Analysis of Antigen-Specific T Cells

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Single cell suspensions of spleens and LNs were stained with Live/Dead Aqua (Life Technologies Corporation, Eugene, OR, USA) along with surface antibodies as indicated as well as with GP33-MHC-I tetramers (NIH, NIAID tetramer core facility, Atlanta, GA, USA) at 4⁰ C for FACS analysis. For intracellular cytokine staining, splenocytes were stimulated with GP33 peptide (200 ng/mL, Peptide 2.0 Inc, Chantilly, VA, USA) for 4 hours in presence of protein transport inhibitor cocktail (eBioscience; Thermo Fisher Scientific, Carlsbad, CA, USA). Cells were stained for surface markers, fixed and permeabilized to stain for intracellular cytokines. Data were analyzed using FlowJo software (Becton Dickinson, Ashland, OR, USA). The following antibodies were used: CD8α (53–6.7), CD62L (MEL-14), Perforin (S16009A), Eomes (Dan11mag), T-bet (ebio4B10), Granzyme B (NGZB) and CD127 (A7R34) from eBioscience (Thermo Fisher Scientific, Carlsbad, CA, USA), KLRG1 (2F1/KLRG1), IFN-γ (XMG1.2), TNF-α (MP6-XT22), CD45.1 (A20), CD45.2 (104) and PD1 (29F.1A12) from BioLegend (San Diego, CA, USA), CD44 (IM7) and CD3ε (145-2C11) from BD PharMingen (BD Biosciences, San Jose, CA, USA).

Jaiswal H., Ciucci T., Wang H., Tang W., Claudio E., Murphy P.M., Bosselut R, & Siebenlist U. (2021). The NF-κB regulator Bcl-3 restricts terminal differentiation and promotes memory cell formation of CD8+ T cells during viral infection. PLoS Pathogens, 17(1), e1009249.

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