Powershot g10 14.7 megapixel digital camera

Excepting specific experiments (see Results section), one-microliter aliquots of fresh samples of tear fluid were taken with a P2-Gilson micropipette fitted with an ultrafine tip and placed sharply on a point of a microscope slide that was positioned horizontally. Tear aliquots were allowed to dry spontaneously at ambient conditions of temperature (range 15-25°C), relative humidity (range 40-45%) and altitude (520 m above sea level). Micrographs of the dry samples were taken under a dark-field microscope (Zeiss Axiostar Plus, objective lens = 2.5X, ocular lens = 10X) fitted with a Canon Powershot G10 14.7 megapixel digital camera. Fern images were classified as types I through IV according to Rolando’s criteria [5 , 6 (link)].

Panels were sampled photographically every 7 days for 8 weeks (ending on 24 September 2019). Panels were pulled out of the water, placed face-up in a plastic box containing dock water, and photographed twice from a distance of approximately 100 mm using a Canon Powershot G10 14.7 megapixel digital camera (Canon Inc., Tokyo, Japan). Sampling using a stereo microscope would have improved the detectability of small organisms, but was not logistically feasible in the field. Panels other than those in the control group were photographed both before and after treatment, unless no relevant organisms were visible to remove (for example, no A. aurita polyps were visible in the first week of sampling). Dissolved oxygen, temperature and salinity were measured each week (except that no salinity measurements were taken in the fifth week) at both 1 and 3 m, using YSI 550 (oxygen) and 556 MPS (temperature and salinity) meters (YSI Inc., Yellow Springs, Ohio, USA). A Secchi disc was visible to at least 3.5 m in every week.

Unless otherwise specified, from each fresh tear sample 1.0-μL aliquots were taken using a 2-μL Gilson micropipette fitted with a ultrafine tip and placed sharply on the center of individual glass microscope slides that had been positioned horizontally. Tear aliquots were allowed to dry spontaneously at ambient conditions (temperature range of 18–25 °C, relative humidity range of 36–40 % and 570 meters above sea level (MASL). Micrographs of the dry specimens, named microdesiccates, were taken using a Zeiss Axiostar Plus microscope (objective lens = 2.5×, ocular lenses = 10×) fitted with a universal 5-position condenser system turret (bright-field, Phases 1, 2 and 3 and dark-field) and with a Canon Powershot G10 14.7 megapixel digital camera. Microdesiccates were routinely prepared in triplicate and classified as types I through IV according to Rolando’s criteria [1 , 10 ].