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Nis 4.0 imaging software

Manufactured by Nikon
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NIS 4.0 is an imaging software developed by Nikon. It is designed to capture, analyze, and manage digital images acquired from various Nikon microscopes and imaging systems.

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Lab products found in correlation

Axioplan 2 microscope, by Nikon (4 mentions) Digital sight ds f12 camera, by Nikon (4 mentions) Lsm 5 exciter axioimager m1 imaging system, by Zeiss (3 mentions) Lsm710 imaging system, by Zeiss (2 mentions) Lsm 710, by Zeiss (2 mentions) Lsm 880, by Zeiss (2 mentions) Lsm510 imaging system, by Zeiss (1 mentions)

4 protocols using nis 4.0 imaging software

1

Confocal Imaging of Electroporated Brain Samples

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Bright-field images were taken using a Zeiss Axioplan 2+ microscope, Nikon Digital Sight DS-F12 camera, and Nikon NIS 4.0 imaging software. Images of immunohistochemistry and immunostaining of electroporated brains and dissociated cultures were obtained using a Zeiss LSM 5 Exciter–AxioImager M1 imaging system and Zeiss LSM710 imaging system. Image stacks were generated by scanning at intervals of 0.5–1 μm using filters of the appropriate wavelengths. The stacks were analyzed, merged, and projected using ImageJ software (RRID: SCR_003070) from the National Institutes of Health. Figure panels were prepared using Adobe Photoshop CS6. Figure 2C,D shows stitched composites from multiple confocal image frames.
Muralidharan B., Keruzore M., Pradhan S.J., Roy B., Shetty A.S., Kinare V., D'Souza L., Maheshwari U., Karmodiya K., Suresh A., Galande S., Bellefroid E.J, & Tole S. (2017). Dmrt5, a Novel Neurogenic Factor, Reciprocally Regulates Lhx2 to Control the Neuron–Glia Cell-Fate Switch in the Developing Hippocampus. The Journal of Neuroscience, 37(46), 11245-11254.
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2

Confocal Imaging of Neuronal Structures

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Bright-field images were taken using a Zeiss Axioplan 2+ microscope, Nikon Digital Sight DS-F12 camera, and Nikon NIS 4.0 imaging software. Images of immunohistochemistry and immunostaining of electroporated brains and dissociated cultures were obtained using a Zeiss LSM 5 Exciter–AxioImager M1 imaging system and Zeiss LSM710 imaging system. Image stacks were generated by scanning at intervals of 0.5–1 μm using filters of the appropriate wavelengths. The stacks were analyzed, merged, and projected using ImageJ software (RRID: SCR_003070) from the National Institutes of Health. Figure panels were prepared using Adobe Photoshop CS6. Figure 2C,D shows stitched composites from multiple confocal image frames.
Muralidharan B., Keruzore M., Pradhan S.J., Roy B., Shetty A.S., Kinare V., D'Souza L., Maheshwari U., Karmodiya K., Suresh A., Galande S., Bellefroid E.J, & Tole S. (2017). Dmrt5, a Novel Neurogenic Factor, Reciprocally Regulates Lhx2 to Control the Neuron–Glia Cell-Fate Switch in the Developing Hippocampus. The Journal of Neuroscience, 37(46), 11245-11254.
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3

Fluorescence Microscopy Imaging Protocol

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Bright-field images were taken using a Zeiss Axioplan 2 + microscope, Nikon Digital Sight DS-F12 camera, and Nikon NIS 4.0 imaging software. Images of immunohistochemistry were obtained using a Zeiss LSM 5 Exciter-AxioImager M1 imaging system and Zeiss LSM510 imaging system. Image stacks were generated by scanning at intervals of 4 μm for lower magnification and at intervals of 0.8 μm for higher magnification using filters of the appropriate wavelengths. The stacks were analyzed, merged, and projected using ImageJ software from the National Institutes of Health. Figure panels were prepared using Adobe Photoshop CS6.
Muralidharan B., Khatri Z., Maheshwari U., Gupta R., Roy B., Pradhan S.J., Karmodiya K., Padmanabhan H., Shetty A.S., Balaji C., Kolthur-Seetharam U., Macklis J.D., Galande S, & Tole S. (2017). LHX2 Interacts with the NuRD Complex and Regulates Cortical Neuron Subtype Determinants Fezf2 and Sox11. The Journal of Neuroscience, 37(1), 194-203.
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4

Microscopy and Image Processing

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Bright-field images were taken using a Zeiss Axioplan 2 + microscope, Nikon Digital Sight DS-F12 camera, and Nikon NIS 4.0 imaging software. Images of immunohistochemistry slides were obtained using a Zeiss LSM 710 and LSM 880 imaging system. Image stacks were processed using ImageJ (NIH) (( 51) and ZEN 2.1 (black) software. Figure panels were prepared using Adobe Photoshop CS6 and Adobe Illustrator.
Pal S., Dwivedi D., Pramanik T., Godbole G., Iwasato T., Jabaudon D., Bhalla U.S., & Tole S. (2020). An early cortical progenitor-specific mechanism regulates thalamocortical innervation.
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