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Z16 microscope

Manufactured by Leica

The Z16 microscope is a high-performance optical instrument designed for a variety of laboratory applications. It features a 16x zoom range, providing versatile magnification capabilities. The Z16 is equipped with a robust, ergonomic design to support extended use in the laboratory setting.

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6 protocols using z16 microscope

1

Identification of Neophyllaphis Aphids

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Individuals preserved in 70% ethanol were cleared using KOH and acetic acid and mounted in slides with Canada balsam. The morphological identification of the specimens was done using a Leica Z16 microscope. We measured structures with taxonomic value and used the keys from Miller and Halbert (2014) (link) and Blackman and Eastop (2019) to identify species of Neophyllaphis. The photographs were taken with a Leica Z16 microscope, equipped with a CF500 camera and LAS 4.9 (Leica) image capture. Mounted specimens were deposited at the aphid collection of the Instituto de Biología Integrativa de Sistemas (Centro Mixto Universidad de Valencia-CSIC, Spain) and in the Centro de Investigación en Biología Celular y Molecular (CIBCM), Universidad de Costa Rica.
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2

Comparative Analysis of Herpetine Beetle Specimens

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Three male specimens of H.rayi were examined and compared to male specimens of H.burnei (three specimens) and H.osburni (one specimen) borrowed from the
Florida Collection of Arthropods, Gainesville, Florida (FSCA).
The holotype and one paratypes of H.rayi MacGown & Hill, sp. nov. were deposited in the
Mississippi Entomological Museum (MEM)
and one paratype will be deposited in the Florida State Collection of Arthropods (FSCA). Genitalia were dissected and examined in 95% ethanol. Photomicrographs were captured using a Leica DFC 495 digital camera mounted on a Leica Z16 microscope with motorized Z-stepping, and image stacks were merged using Leica Application Suite v. 4.1.0 with Montage Module. All images were edited in Photoshop CS6. Measurements were made using a reticule placed in a 10× eyepiece of a Leica MZ16 stereomicroscope at a magnification of 10–100×.
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3

Identification of Invasive Fire Ant Species

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Specimens from possible fire ant mounds reported by Kentucky citizens were collected by CAPS employees and placed in ethanol and/or hexane and sent to the MEM Invasive Insect Screening Center for identification and verification. Identifications were made using morphological methods and the keys found in [1 ]. When possible, identifications were verified using DNA analysis based on methods provided by Goodisman et al. [3 (link)] and using gas chromatography and mass spectrometer (GC/MS) methods to separate S. invicta, S. richteri, and their hybrid from one another based on different cuticular hydrocarbon and venom alkaloid profiles as described in Menzel et al. [2 ]. Specimens collected in ethanol were adequate for both DNA and morphological-based identifications, whereas samples stored in hexane were necessary for the GC/MS analyses. Photomicrographs were captured using a Leica DFC 495 digital camera mounted on a Leica Z16 Microscope with motorized Z-stepping, and image stacks were merged using Leica Application Suite V 4.1.0 with the Montage Module. All images were edited in Photoshop CS6. Voucher specimens were deposited in the MEM.
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4

Microscopy Imaging Protocol for Wasps

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At SAMC we used a Leica LAS 4.4 imaging system, which comprised a Leica® Z16 microscope with a Leica DFC450 Camera with 0.63× video objective attached. The PageBreakimaging process, using an automated Z-stepper, was managed using the Leica Application Suite V 4.4 software installed on a desktop computer. At BMNH, images were acquired using a Canon SLR EOS 5DSR with 65 mm macro lens mounted on a copy stand with an automated Z-stepper; images were aligned using Helicon Focus software version 6.6.1. Diffused lighting was achieved using techniques summarized in Buffington et al. (2005) , Kerr et al. (2008) , and Buffington and Gates (2009) . All images presented in this paper, as well as supplementary images, are available at www.waspweb.org.
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5

Identification of Weevil Specimens

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The bulbs sent to the MEM were carefully dissected to reveal two adult weevils. The specimens were imaged using a Leica DFC 495 digital camera mounted on a Leica Z16 microscope and Leica Application Suite was used to create image stacks (Figure 2). The images were then sent to Lourdes Chamorro at the USNM to help with the identification of the weevils. One specimen is deposited at the MEM and the other at the USNM. The following resources were used to identify this weevil: Zimmerman [16 ] and authoritatively identified specimens housed in the USNM.
Records from the Systematic Entomology Laboratory’s Communications & Taxonomic Service Unit (CTSU) internal identification system were queried in 2016 for information on interception records.
The distribution map was made using QGIS [17 ] with data from specimens at the USNM and from specimens found in the Symbiota Collections of Arthropods Network (SCAN) database [18 ]. MycoBank.org [19 ] was used to check the names of the fungal pathogens.
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6

Insect Specimen Mounting and Imaging

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Specimens were point mounted on black, acid-free cards for examination (using a Leica M205C stereomicroscope with LED light source), photography and long-term preservation. Images were acquired using either the EntoVision® multiple-focus imaging system or the Leica LAS 4.4 imaging system. The EntoVision® system comprised a PageBreakLeica® M16 microscope with a JVC® KY–75U 3–CCD digital video camera attached that fed image data to a notebook computer. The program Cartograph® 5.6.0 was used to manage image acquisition using an automated Z-stepper and merging of the image series into a single in-focus image. The Leica LAS 4.4 system comprised a Leica® Z16 microscope with a Leica DFC450 Camera with a 0.63× video objective attached. Leica Application Suite V 4.4 software was installed on a desk top computer. Lighting was achieved using techniques summarized in Buffington et al. (2005) , Kerr et al. (2008) and Buffington and Gates (2009) . All images presented in this paper are available at http://www.waspweb.org.
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