Radioactive isotopes

Oligonucleotides were from Integrated DNA Technologies (Coralville, IA). All chemicals were from Fisher Scientific (Pittsburgh, PA). DNA was sequenced by Functional Biosciences (Madison, WI). Radioactive isotopes were from PerkinElmer (Waltham, MA). Escherichia coli tolC mutant, P. aeruginosa PAO200 (efflux pump mutant), and P. aeruginosa hypersensitive strain (ATCC 35151) were a kind gift from Urs Ochsner (Crestone Pharma, Boulder, CO). All other bacteria were from the American Type Culture Collection (ATCC, Manassas, VA). The natural product library was from MicroSource Discovery Systems, Inc. (Gaylordsville, CT), and the synthetic compound library was from TimTec LLC (Newark, DE). Compounds stocks were dissolved in DMSO to a concentration of 10 mM, stored at −20 °C, and thawed immediately before analysis. The compounds have an average purity of 95%, and the minimum purity is at least 90%.

All chemicals were obtained from Fisher Scientific (Pittsburg, PA). Radioactive isotopes, SPA beads and 96-well screening plates were from PerkinElmer (Waltham, MA). E. coli tolC mutant, P. aeruginosa PAO200 (efflux pump mutant) and P. aeruginosa hypersensitive (ATCC^® 35151^™) strains were a kind gift from Urs Ochsner (Crestone Pharma, Boulder, CO). All other bacteria were from American Type Culture Collection (ATCC) (Manassas, VA). The synthetic compound library was from TimTec LLC (Newark, DE) and the natural product library was from MicroSourceDiscovery Systems, Inc. (Gaylordsville, CT). Compounds were supplied as 10 mM stocks dissolved in dimethyl sulfoxide (DMSO), stored at −20 °C and thawed immediately before analysis. The compounds have an average purity of 95%, and the minimum purity is at least 90%.

Oligonucleotides were from Integrated DNA Technologies (Coralville, IA). All chemicals were from Fisher Scientific (Pittsburg, PA). DNA was sequenced by Functional Biosciences (Madison, WI). Radioactive isotopes were from PerkinElmer (Waltham, MA). tRNA was from E. coli MRE 600 (Roche Diagnostics, Mannheim, Germany). The E. coli tolC mutant strain, P. aeruginosa PAO200 (efflux pump mutant) and P. aeruginosa hypersensitive strain (ATCC^® 35151^™) were a kind gift from Urs Ochsner (Crestone Pharma-Boulder, CO). All other bacteria were from American Type Culture Collection (ATCC) (Manassas, VA). The natural product libraries were from MicroSource Discovery Systems, Inc. (Gaylordsville, CT) and Prestwick Chemical, Inc. (Strasbourg-Illkirch, France), and the synthetic compound library was from TimTec LLC (Newark, DE). Compounds stocks were dissolved in dimethyl sulfoxide (DMSO) to a concentration of 10 mM, stored at −20 °C and thawed immediately before analysis. The compounds have an average purity of 95%, and the minimum purity is at least 90%.

All chemicals were obtained from either Sigma Aldrich (St. Louis, MO) or Fisher Scientific (Pittsburgh, PA). Radioactive isotopes, SPA beads, and 96-well screening plates were from PerkinElmer (Waltham, MA). Escherichia coli tolC mutant, P. aeruginosa PAO200 (efflux pump mutant), and P. aeruginosa hypersensitive (ATCC 35151) strains were a kind gift from Urs Ochsner (Crestone Pharma, Boulder, CO). All other bacteria were from the American Type Culture Collection (ATCC) (Manassas, VA). Penicillin-streptomycin solution was from Mediatech, Inc. (Centerville, IA). The synthetic Anti-Infectives Library was from TimTec LLC (Newark, DE) and included 890 low-molecular-weight, druglike molecules with scaffolds found in antiseptic agents with antibacterial, antifungoid, and antimicrobial activities. Compounds were supplied as 10 mM stocks dissolved in DMSO, stored at −20 °C, and thawed immediately before analysis. The compounds have an average purity of 95%, and the minimum purity is at least 90%.

All chemicals were obtained from Fisher Scientific (Pittsburgh, PA). DNA oligonucleotides were from Integrated DNA Technologies (Coralville, IA). DNA sequencing was performed by Functional Bioscience (Madison, WI). Radioactive isotopes, SPA beads, and 96-well screening plates were from PerkinElmer (Waltham, MA). The Soluble Diversity chemical compound library was from ChemDiv, Inc. (San Diego, CA). Compounds stocks were dissolved in DMSO to a concentration of 10 mM, stored at −20 °C, and thawed immediately before analysis. The compounds had a minimum purity of at least 90%.

Oligonucleotides were from Integrated DNA Technologies (Coralville, IA). All other chemicals were from Fisher Scientific (Pittsburgh, PA). DNA sequencing was performed by Functional Bioscience (Madison, WI). Radioactive isotopes were from PerkinElmer (Waltham, MA). Escherichia coli tolC mutant, P. aeruginosa PAO200 (efflux pump mutant), and P. aeruginosa hypersensitive strain (ATCC 35151) were a kind gift from Urs Ochsner (Crestone Pharma, Boulder, CO). All other bacteria were from the American Type Culture Collection (ATCC) (Manassas, VA). Penicillin-streptomycin solution was from Mediatech, Inc. (Manassas, VA). The synthetic compound library was from TimTec LLC (Newark, DE), and the natural compound library was from MicroSource Discovery Systems (Gaylordsville, CT). All compounds were provided at or above 95% homogeneity.

All chemical compounds used in this study were obtained from Fisher Scientific (Pittsburg, PA). Radioactive isotopes, SPA beads, and 96-well plates were obtained from PerkinElmer (Waltham, MA). The synthetic compound library was from TimTec LLC (Newark, DE), and the natural product library was from MicroSourceDiscovery Systems, Inc. (Gaylordsville, CT). The chemical compounds were obtained as 10 mM stock solutions dissolved in DMSO and stored at −20 °C. These compounds were thawed immediately before screening. The compounds have an average purity of 95%, with the minimum purity at least 90%.