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Unity 400 400 mhz nmr spectrometer

Manufactured by Agilent Technologies
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The Unity-400 (400 MHz) NMR spectrometer is a nuclear magnetic resonance (NMR) spectrometer designed by Agilent Technologies. It operates at a frequency of 400 MHz and is used for the analysis and characterization of chemical compounds.

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Lab products found in correlation

Cary 300 spectrometer, by Agilent Technologies (1 mentions) F 7000 spectrofluorometer, by Hitachi (1 mentions) Su8100 scanning electron microscope, by Hitachi (1 mentions)

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3 protocols using unity 400 400 mhz nmr spectrometer

1

Molecular Weight Analysis of Alginates

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Molecular weights of alginates and PEG-alginate were analyzed with a Malvern Viscotek 270max GPC equipped with a GPCmax solvent and sample delivery module, an Eldex Ch-150 temperature-controlled column holder, a VE 3580 refractive index (RI) detector, viscotek 270 Dual Detector featuring intrinsic viscosity (IV-DP) and right angle light scattering (RALS), and OmniSec software. Samples were dissolved in 0.1 M NaNO3 buffer solution at a concentration of 5 mg/mL, and 200 μL of sample was injected. Polymers separated through a set of two TSK-gel columns (G4000PWXL and G3000PWXL) were analyzed with the triple detector system. Malvern PEO and pullulan standards were used in molecular weight calculation, and weight average molecular weights (Mw) were used.
High-resolution 1H NMR spectra were obtained in deuterium oxide (D2O) using a Varian Unity-400 (400 MHz) NMR spectrometer (Varian). 1H NMR was used to characterize PEG coupling of alginate and degree of functionalization of PEG-alginate.
Chaudhuri O., Gu L., Klumpers D., Darnell M., Bencherif S.A., Weaver J.C., Huebsch N., Lee H.P., Lippens E., Duda G.N, & Mooney D.J. (2015). Hydrogels with tunable stress relaxation regulate stem cell fate and activity. Nature materials, 15(3), 326-334.
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2

Characterization of 5-(4-aminophenyl)-10,15,20-triphenylporphyrin

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UV-Vis spectra were collected on a Cary 300 spectrometer. IR spectra were recorded on a Nicolet iS50 spectrometer using KBr pellets in the region of 4000–500 cm−1. 1H NMR spectra were recorded on a Varian Unity 400 (400 MHz) NMR spectrometer. Chemical shifts were reported on the d-scale relative to tetramethylsilane (TMS). Mass spectra were obtained using a Brucker Autoflex speed TOF/TOF Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry. Thermal analysis was recorded on Perkin-Elmer TG-7 apparatus (sample: 3–4 mg, heating rate: 10 °C/min, and nitrogen atmosphere). Fluorescence spectra were recorded with a HITACHI F-7000 spectrofluorometer. SEM figures were collected by the HITACHI SU8100 scanning electron microscope. A single-spindle electrostatic spinning machine was used to prepare electrospun fibers.
The reagents and solvents used in the experiment were of the commercial analytical pure grade and were used without further purification. The 5-(4-aminophenyl)-10,15,20-triphenylporphyrin was synthesized in the laboratory according to [21 (link)].
Sun E.J., Bai X.Y., Chang Y., Li Q., Hui X.R., Li Y.S, & Wang Y. (2022). Preparation of PMMA Electrospun Fibers Bearing Porphyrin Pendants and Photocatalytic Degradation of Organic Dyes. Molecules, 27(23), 8132.
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3

Molecular Weight Analysis of Alginates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Molecular weights of alginates and PEG-alginate were analyzed with a Malvern Viscotek 270max GPC equipped with a GPCmax solvent and sample delivery module, an Eldex Ch-150 temperature-controlled column holder, a VE 3580 refractive index (RI) detector, viscotek 270 Dual Detector featuring intrinsic viscosity (IV-DP) and right angle light scattering (RALS), and OmniSec software. Samples were dissolved in 0.1 M NaNO3 buffer solution at a concentration of 5 mg/mL, and 200 μL of sample was injected. Polymers separated through a set of two TSK-gel columns (G4000PWXL and G3000PWXL) were analyzed with the triple detector system. Malvern PEO and pullulan standards were used in molecular weight calculation, and weight average molecular weights (Mw) were used.
High-resolution 1H NMR spectra were obtained in deuterium oxide (D2O) using a Varian Unity-400 (400 MHz) NMR spectrometer (Varian). 1H NMR was used to characterize PEG coupling of alginate and degree of functionalization of PEG-alginate.
Chaudhuri O., Gu L., Klumpers D., Darnell M., Bencherif S.A., Weaver J.C., Huebsch N., Lee H.P., Lippens E., Duda G.N, & Mooney D.J. (2015). Hydrogels with tunable stress relaxation regulate stem cell fate and activity. Nature materials, 15(3), 326-334.
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