Anti tead

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-TEAD is a primary antibody that recognizes the TEAD transcription factor. TEAD is a key regulator of gene expression involved in various cellular processes. The anti-TEAD antibody can be used to detect and analyze the TEAD protein in cells and tissues using techniques such as Western blotting, immunohistochemistry, and immunofluorescence.

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Anti-pan-Tead (2 citations)

3 protocols using anti tead

Western Blotting of PTEN and Akt Pathway

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Western blotting was performed according to standard method described previously [25 (link)] using the following antibodies: anti-PTEN (#9559; 1:1000), anti-phosphorylated (p)-PTEN (Ser380/Thr382/383) (#9554; 1:1000), anti-Akt (#4691; 1:1000), anti-p-Akt (Ser473) (#4060; 1:1000), anti-p-Akt (Thr308) (#13088; 1:1000), anti-Bad (#9239; 1:1000), anti-p-Bad (Ser136) (#4366; 1:1000), anti-p-FoxO1 (Ser256) (#9461; 1:1000), anti-FoxO1(#2880; 1:1000), anti-p27 Kip1 (#3686; 1:1000), anti-p-GSK-3β (#9323; 1:1000), anti-GSK-3β (#9315; 1:1000), anti-p-YAP (Ser 127) (#13008; 1:1000), anti-YAP/TAZ(#8418; 1:1000), anti-SAV1 (#13301; 1:1000), anti-LATS1 (#3477; 1:1000), anti-LATS2 (#5888; 1:1000), anti-MOB1 (#8699; 1:1000), anti-histone H3 (#4499; 1:1000), and anti-TEAD (#13295; 1:1000) from Cell Signaling Technology (Danvers, MA, USA) and anti-GAPDH (BA2913, 1:1000) from Boster Biological Technology (Preston, CA, USA).

Xu W., Yang Z., Xie C., Zhu Y., Shu X., Zhang Z., Li N., Chai N., Zhang S., Wu K., Nie Y, & Lu N. (2018). PTEN lipid phosphatase inactivation links the hippo and PI3K/Akt pathways to induce gastric tumorigenesis. Journal of Experimental & Clinical Cancer Research : CR, 37, 198.

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Protein Expression Analysis in Cell Lines

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Total cell lysates and cytoplasmic and nuclear protein fractions were extracted from cell cultures as described previously [51 (link)]. Standard Western blot analysis of protein expression was carried out using primary anti-HA (rabbit; Cell Signaling Technology), anti-TAZ (rabbit; Cell Signaling Technology), anti-YAP (rabbit; Cell Signaling Technology), anti-E-cadherin (mouse; BD Biosciences), anti-vimentin (rabbit; Cell Signaling Technology), anti-Merlin (rabbit; Santa Cruz Biotechnology), anti-LATS1 (rabbit; Cell Signaling Technology), anti-MST1 (rabbit; Cell Signaling Technology), anti-phosphorylated LATS1 (Thr1079, rabbit; Cell Signaling Technology), anti-phosphorylated MST1 (Thr183)/MST2 (Thr180, rabbit; Cell Signaling Technology), anti-TEAD (rabbit; Cell Signaling Technology), and anti-CTGF (mouse; Santa Cruz Biotechnology) antibodies. Equal protein-sample loading was monitored using an anti- glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody for total cell protein lysates (rabbit; Santa Cruz Biotechnology), an anti-α-tubulin antibody for cytoplasmic fractions (mouse; Oncogene), and an anti-lamin B1 antibody for nuclear fractions (goat; Santa Cruz Biotechnology).

Xie D., Cui J., Xia T., Jia Z., Wang L., Wei W., Zhu A., Gao Y., Xie K, & Quan M. (2015). Hippo transducer TAZ promotes epithelial mesenchymal transition and supports pancreatic cancer progression. Oncotarget, 6(34), 35949-35963.

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Western Blot and ChIP-seq Antibodies

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For western blot analysis, proteins were detected using the following reagents: anti-GAPDH (Cell Signaling Technology, 97166, 1:1,000), anti-b-Actin (Cell Signaling Technology, 3700, 1:1000) anti-FLAG (Cell Signaling Technology, 2368, 1:1,000), anti-MYC (Invitrogen, A21281, 1:1,000), anti-TEAD (Cell Signaling Technology, 13295, 1:1,000), anti-Rabbit (LI-COR, 926-68071, 1:15,000), anti-Chicken (LI-COR, 926-32218, 1:15,000), anti-Mouse (LI-COR, 925-32210, 1:15,000), streptavidin (LI-COR, 925-32230, 1:15,000). For ChIP-seq analysis, anti TEF1/TEAD-1 (Abcam, ab133533) was used.

Holden J.K., Crawford J.J., Noland C.L., Schmidt S., Zbieg J.R., Lacap J.A., Zang R., Miller G.M., Zhang Y., Beroza P., Reja R., Lee W., Tom J.Y.K., Fong R., Steffek M., Clausen S., Hagenbeek T.J., Hu T., Zhou Z., Shen H.C, & Cunningham C.N. (2020). Small Molecule Dysregulation of TEAD Lipidation Induces a Dominant-Negative Inhibition of Hippo Pathway Signaling. Cell reports, 31(12).

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