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Goat anti runx2

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Goat anti-Runx2 is a polyclonal antibody that specifically recognizes the Runx2 protein. Runx2 is a transcription factor that plays a crucial role in osteoblast differentiation and bone formation. This antibody can be used for the detection and analysis of Runx2 expression in various biological samples.

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Lab products found in correlation

Alexa fluor 488 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions) Fluoview 1000, by Olympus (1 mentions) Hoechst, by Merck Group (1 mentions) Anti pcna antibody, by Santa Cruz Biotechnology (1 mentions) Rabbit anti klf10, by Merck Group (1 mentions) Rabbit anti myc, by Abcam (1 mentions) Monoclonal anti flag hrp, by Merck Group (1 mentions) Goat anti lamin b, by Santa Cruz Biotechnology (1 mentions) Rabbit anti hif 2α, by Santa Cruz Biotechnology (1 mentions) Abc standard kit, by Vector Laboratories (1 mentions) Rabbit anti sirt1, by Proteintech (1 mentions) Quantity one software, by Bio-Rad (1 mentions) Rabbit anti erk1 2, by Cell Signaling Technology (1 mentions) Bmp 2, by Thermo Fisher Scientific (1 mentions) Goat anti ddr2, by R&D Systems (1 mentions)

Close spelling variants of this product

Anti-Runx2 (37 citations) RUNX2 (76 citations)

6 protocols using goat anti runx2

1

Immunofluorescence Analysis of Osteogenic Markers

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The D1 cells were seeded on glass coverslips. At the end of the experiment, cells were fixed for 15 minutes in 4% paraformaldehyde containing 0.5% Triton-X-100 and preincubated in 100 µL of blocking solution (2.5% bovine serum albumin in phosphate buffered saline) for 40 minutes. The cytoskeleton was detected using 0.5 µM Alexa Fluor 488-conjugated phalloidin (Molecular Probes, Invitrogen, Basel, Switzerland) and DAPI for 1 hour. Osteogenic proteins were labeled by incubation with goat anti-Runx2 (Santa Cruz Biotechnology Inc., Dallas, TX, USA), goat anti-BMP-2 (Santa Cruz Biotechnology Inc.), and rabbit antiosteocalcin (Santa Cruz Biotechnology Inc.) overnight. Cells were rinsed five times with PBST and stained with antigoat IgG conjugated with Alexa 638 (Molecular Probes) or antirabbit IgG conjugated with Alexa 638 (Molecular Probes) combined with 0.5 µM Alexa Fluor 488-conjugated phalloidin (Molecular Probes), and DAPI for 1 hour. Cells were rinsed five times in phosphate buffered saline with 0.01% Triton X-100 (PBST). Glass coverslips were fixed to the slides by using a mounting medium (35 µL) and sealed with nail polish. Images were observed using a confocal microscope (FluoView 1000; Olympus Corporation, Tokyo, Japan) at 60×.
Tai I.C., Wang Y.H., Chen C.H., Chuang S.C., Chang J.K, & Ho M.L. (2015). Simvastatin enhances Rho/actin/cell rigidity pathway contributing to mesenchymal stem cells’ osteogenic differentiation. International Journal of Nanomedicine, 10, 5881-5894.
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2

Immunohistochemistry of Osteogenic Markers

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Cells were fixed in cold acetone and methanol (1:1), permeabilized in 0.2 % Triton X-100 at ice for 30 min and blocked with 10 % goat serum for 30 min at RT. For double-labeling, two primary antibodies were incubated simultaneously overnight at 4 °C at the following dilutions: rabbit anti-Klf10 (1:100; Sigma-Aldrich), goat anti-Dsp (1:100; Santa Cruz Biotechnology), goat anti-Runx2 (1:100; Santa Cruz Biotechnology) and mouse anti-Dmp1 (1:100; kind gift from Dr. Chunlin Qin, Texas A&M University, Baylor College of Dentistry, Tex., USA; Qin et al. 2006 (link)). After being washed, slides were incubated with the secondary antibody conjugated with Alexa Fluo 486 green and Alexa Fluo 568 red (1:500; Molecular Probes) for 1 h at RT. As a negative control, the primary Klf10 antibody was replaced by mouse IgG I. For nuclear staining, the cells were treated with Hoechst (Sigma-Aldrich). Slides were viewed and captured by using a Nikon inverted microscope. For double-fluorescent immunohistochemistry, the co-expression of Klf10 and PCNA (proliferating cell nuclear antigen) was analyzed in tissue sections from various mouse ages. Anti-PCNA antibody was purchased from Santa Cruz Biotechnology.
Chen Z., Li W., Wang H., Wan C., Luo D., Deng S., Chen H, & Chen S. (2015). Klf10 regulates odontoblast differentiation and mineralization via promoting expression of dentin matrix protein 1 and dentin sialophosphoprotein genes. Cell and tissue research, 363(2), 385-398.
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3

Immunodetection of Cell Markers

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Rabbit anti-HA, Rabbit anti-Hif-2α, goat anti-Runx2, monoclonal anti-actin and goat anti-Lamin B antibodies were purchased from Santa Cruz Biotechnology (CA, USA). Rabbit anti-Myc and monoclonal anti-Runx2 were purchased from Abcam (MA, USA). Monoclonal anti-Flag, Monoclonal anti-Flag-HRP, HA-anti HA-agarose antibody and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Che X., Park N.R., Jin X., Jung Y.K., Han M.S., Park C.Y., Chun J.S., Kim S.G., Jin J., Kim H.J., Lian J.B., Stein J.L., Stein G.S, & Choi J.Y. (2021). Hypoxia-inducible factor 2α is a novel inhibitor of chondrocyte maturation. Journal of cellular physiology, 236(10), 6963-6973.
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4

Osteoblastic Differentiation Assessment via Runx2 IHC

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To assess osteoblastic differentiation, we conducted an immunohistochemical test by utilizing a primary polyclonal goat antibody against Runx 2 (Goat anti-Runx2—Santa Cruz Biotechnology, SC8566, Santa Cruz, CA, USA) [25 (link)]. The team employed the immunoperoxidase detection method and amplified the signal from the reaction through incubation in avidin and biotin (ABC standard kit, Vector Laboratories, Burlingame, CA, USA). For the reaction revelation, we used Diaminobenzidine (Dako Laboratories, Santa Clara, CA, USA) and performed counterstaining with Harris hematoxylin. After dehydration through xylene, the researchers mounted coverslips over the sections to evaluate and utilize a scoring system in accordance with dos Santos Pereira et al. [26 (link)], which was performed by a single evaluator who had been previously calibrated. Scores were in the range of 0 (absence of staining), 1 (low staining), 2 (moderate staining), and 3 (intense staining).
de Souza Santos A.M., dos Santos Pereira R., Montemezzi P., Mello-Machado R.C., Okamoto R., Sacco R., Noronha Lisboa-Filho P., Messora M.R., Mourão C.F, & Hochuli-Vieira E. (2023). The Interplay of Raloxifene and Sonochemical Bio-Oss in Early Maxillary Sinus Bone Regeneration: A Histological and Immunohistochemical Analysis in Rabbits. Medicina, 59(9), 1521.
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5

Western Blot Analysis of Sirt1, Runx2

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All samples were collected, and the Western blot was conducted as previously described [29] . The primary antibodies used were rabbit anti-Sirt1 (1:2000; Proteintech, Rosemont,USA), goat anti-Runx2 (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and mouse anti-GAPDH (1:10,000; Kangcheng, Shanghai, China). The protein bands were quantitatively analyzed using an image analysis system (QuantityOne software; Bio-Rad, Hercules, CA, USA).
Hong W., Wei Z., Qiu Z., Li Z., Fu C., Ye Z., & xu x. (2020). Atorvastatin Promotes Bone Formation in Aged apoE–/– Mice through the Sirt1–Runx2 Axis.
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6

Osteogenic Differentiation Assay

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Goat anti-DDR2 (R&D Systems, MN, USA), rabbit anti-Nrp1, mouse anti-β-actin (Abcam, MA, USA), goat anti-Runx2 (Santa Cruz, TEX, USA), rabbit anti-ERK1/2 (Cell Signaling Technology, MA, USA). BMP-2 (Peprotech, NJ, USA), Ascorbic acid (AA), β-glycerophosphate (β-GP) (Sigma, IL, USA).
Zhang Y., Su J., Teng Y., Zhang J., Wang J., Li K., Yao L, & Li X. (2015). Nrp1, a Neuronal Regulator, Enhances DDR2-ERK-Runx2 Cascade in Osteoblast Differentiation via Suppression of DDR2 Degradation. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 36(1).
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