Cardiac ILC2s were isolated using FACSAria II (BD Biosciences) from the hearts of IL-33-treated mice and cultured at 37°C in RPMI 1640 (GIBCO) with 10% FBS (GE Healthcare Life Sciences), 1% Penicillin-Streptomycin, 2 mM L-Glutamine, 10 mM HEPES, 1 mM Sodium Pyruvate (all Quality Biological), 0.1 mM Non-essential amino acids (Sigma Aldrich), 0.05 mM 2-Mercaptoethanol (GIBCO). Cytokines, IL-2, IL-7 and IL-33, at a concentration of 25 ng/ml were added in media to expand ILC2s in vitro.
Sodium pyruvate
Manufactured by Quality Biological
Sourced in United States
Sodium Pyruvate is a chemical compound commonly used in cell culture media as an energy source for cells. It serves as a substrate for various metabolic processes, supporting cell growth and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Quality Biological (3 mentions)
L glutamine, by Quality Biological (3 mentions)
Non essential amino acids (neaa), by Merck Group (2 mentions)
Fetal bovine serum (fbs), by GE Healthcare (2 mentions)
Hepes, by Quality Biological (2 mentions)
2 mercaptoethanol, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Facsaria 2, by BD (2 mentions)
Rpmi 1640, by Quality Biological (1 mentions)
Pc12 cell line, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Quality Biological (1 mentions)
3 protocols using sodium pyruvate
1
Isolation and Culture of Cardiac ILC2s
2
Isolation and Culture of Cardiac ILC2s
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Cardiac ILC2s were isolated using FACSAria II (BD Biosciences) from the hearts of IL-33-treated mice and cultured at 37°C in RPMI 1640 (GIBCO) with 10% FBS (GE Healthcare Life Sciences), 1% Penicillin-Streptomycin, 2 mM L-Glutamine, 10 mM HEPES, 1 mM Sodium Pyruvate (all Quality Biological), 0.1 mM Non-essential amino acids (Sigma Aldrich), 0.05 mM 2-Mercaptoethanol (GIBCO). Cytokines, IL-2, IL-7 and IL-33, at a concentration of 25 ng/ml were added in media to expand ILC2s in vitro.
3
Culturing and Maintaining PC-12 Cells
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The PC-12 cell line, which is derived from rat adrenal medulla, was obtained from American Type Culture Collection (Manassas, VA, USA). The PC-12 cells were maintained in RPMI-1640 (Quality Biological, Gaithersburg, MD, USA) supplemented with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer [1 mM, pH 7.4] (Mediatech, Inc., Manassas, VA, USA), 10% heat-inactivated horse serum (Biosource, Rockville, MD, USA), 5% fetal bovine serum (FBS), 1% sodium pyruvate, 5% L-glutamine and 1% penicillin/streptomycin all purchased from Quality Biological.
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